Comparative Anti-plasmodial and Cytotoxic Effect of the Methanolic Extracts of Leaves, Stem Bark and Roots of Annickia affinis
International Journal of Biochemistry Research & Review, Volume 32, Issue 1,
Aims: This study aimed to evaluate and compare the in vitro anti-plasmodial and cytotoxic effect of the methanolic extracts from leaves, stem bark and roots of Annickia affinis.
Study Design: This is an experimental study.
Place and Duration of Study: The work was conducted at the Pharmacochemistry and Natural Substances Laboratories of the Faculty of Medicine and Pharmaceutical Sciences, University of Douala for the extraction and at the Biomedical Chemistry Research Center of Rhodes University in South Africa for the antimalarial and cytotoxic essay. All the experiments were carried out from the 15th October 2019 to the 31th July 2020.
Methodology: The anti-plasmodial test was performed on Plasmodium falciparum sensitive strains 3D7 while cytotoxicity was evaluated on the HeLa cell line.
Results: The anti-plasmodial tests revealed that the roots and the stem bark exhibited a moderate anti-plasmodial effect with IC50 of 19.7 ± 0.8 and 12.1 ± 0.8 µg/ml respectively. The anti-plasmodial effects of the leaves were classified as low (33.7 ± 1.9 µg/ml). At up to 50 µg/ml, all the extracts showed a high rate of survival among the HeLa cells. No effect was observed with the leaf extracts (100% of survival).
Conclusion: This is the first report on the cytotoxic study and comparative anti-plasmodial effect of Annikia affinis. It highlights the potential of Annickia affinis as an important source of anti-plasmodial drugs with less cytotoxic in vitro. In agreement with the use in traditional medicine, the stem bark was more active than wood, while leaves showed low activity.
- Annickia affinis
- anti-plasmodial activity
- Plasmodium falciparum 3D7
- medicinal plant
How to Cite
WHO. Word Malaria Report 2021; 2021.
Mustofa, Valentin A, Benoit-Vical F, Pélissier Y, Koné-Bamba D, Mallié M. Antiplasmodial activity of plant extracts used in west African traditional medicine. J Ethnopharmacol. 2000;73(1-2):1451. DOI: 10.1016/S0378-8741(00)00296-8
Ene AC, Atawodi SE, Ameh DA, Kwanashie HO, Agomo PU. In vivo antiplasmodial effect of chloroform extracts of Artemisia maciverae Linn and Artemisia maritima Linn. Afr J Biotechnol. 2009; 8(23):66126. DOI: 10.4314/ajb.v8i23.66362
Bagavan A, Rahuman AA, Kamaraj C, Kaushik NK, Mohanakrishnan D, Sahal D. Antiplasmodial activity of botanical extracts against Plasmodium falciparum. Parasitol Res. 2011;108(5):1099-109. DOI: 10.1007/s00436-010-2151-0
Inbaneson SJ, Ravikumar S, Suganthi P. In vitro antiplasmodial effect of ethanolic extracts of coastal medicinal plants along Palk Strait against Plasmodium falciparum. Asian Pac J Trop Biomed. 2012;2(5):3647. DOI: 10.1016/S2221-1691(12)60057-4
Donkor AM, Oduro-Mensah D, Ani E, Ankamah E, Nsiah S, Mensah ED et al. In vitro anti-plasmodial activity of aqueous and ethanolic extracts of Moringa oleifera and Phyllanthus amarus. Int J Biol Chem. 2015;9(4):198-206. DOI: 10.3923/ijbc.2015.198.206
Nworu CS, Ejikeme, Ezike AC, Ndu O, Akunne CT, Onyeto AC et al. Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & s.) D.C. (Rubiaceae). Afr Health Sci. 2017;17(3): 8273. DOI: 10.4314/ahs.v17i3.26
Berthi W, González A, Rios A, Blair S, Cogollo Á, Pabón A. Anti-plasmodial effect of plant extracts from Picrolemma huberi and Picramnia latifolia. Malar J. 2018; 17(1):1-12. DOI: 10.1186/s12936-018-2301-x
Mbosso TJE, Siwe NX, Nguemfo EL, Meyer F, Wintjens R, Isaacs M et al. Biological activities of plant extracts from Ficus elastica and Selaginella vogelli: An antimalarial, antitrypanosomal and cytotoxity evaluation. Saudi J Biol Sci. 2018;25(1):117-22. DOI: 10.1016/j.sjbs.2017.07.002
Asmare M, Mohammedbrhan A, Dessalegn A, Gelayee A. In-vivo anti-malarial activity of crude extract and solvent fractions of the roots of Clematis simensis Fresen (Ranunculaceae) in Plasmodium berghei infected mice. J Pharm Biol Sci. 2019;14(5):52-62. DOI: 10.9790/3008-1405015262
Fenta M, Kahaliw W. Evaluation of antimalarial activity of hydromethanolic crude extract and solvent fractions of the leaves of Nuxia congesta R. Br. Ex fresen (Buddlejaceae) in Plasmodium berghei infected mice. J Exp Pharmacol. 2019;11: 121-34. DOI: 10.2147/JEP.S230636
Fouokeng Y, Feumo HM, Mbosso TJE, Siwe NX, Wintjens R, Isaacs M, et al. In vitro antimalarial, antitrypanosomal and HIV-1 integrase inhibitory activities of two Cameroonian medicinal plants: Antrocaryon klaineanum (Anacardiaceae) and Diospyros conocarpa (Ebenaceae). South Afr J Bot. 2019;122:510-7. DOI: 10.1016/j.sajb.2018.10.008
Mbosso TJE, Siwe NX, Zeh MJE, Meyer F, Tabouguia MO, Assob NJC et al. Compound isolation and biological activities of Piptadeniastrum africanum (hook.f.) Brennan roots. J Ethnopharmacol. 2020;255:112716. DOI: 10.1016/j.jep.2020.112716
Okaiyeto K, Okoh AI. In vitro assessment of antiplasmodial and antitrypanosomal activities of chloroform, ethyl acetate and ethanol leaf extracts of Oedera genistifolia. Appl Sci. 2020;10(19):1-13. DOI: 10.3390/app10196987
Atsbha GH, Balasubramanian R, Gebre AK. Antimalarial effect of the root of Silene macrosolen A. Rich (Caryophyllaceae) on Plasmodium Berghei-Infected Mice. Evidence-based Complement Altern Med. 2021;2021(2):1-11. DOI: 10.1155/2021/8833865
Tajbakhsh E, Kwenti TE, Kheyri P, Nezaratizade S, Lindsay DS, Khamesipour F. Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: A systematic review of literature. Malar J. 2021;20:349-98 DOI: 10.1186/s12936-021-03866-0
Chaniad P, Phuwajaroanpong A, Techarang T, Viriyavejakul P, Chukaew A, Punsawad C. Antiplasmodial activity and cytotoxicity of plant extracts from the Asteraceae and Rubiaceae families. Heliyon. 2022;8(1):e08848. DOI: 10.1016/j.heliyon.2022.e08848
Erhunse N, Omoregie SE, Sahal D. Antiplasmodial and antimalarial evaluation of a Nigerian hepta-herbal Agbo-iba decoction: Identification of magic bullets and possible facilitators of drug action. J Ethnopharmacol. 2023;301:115807. DOI: 10.1016/j.jep.2022.115807
Olivier DK, Van Vuuren SF, Moteetee AN. Annickia affinis and A. chlorantha (Enantia chlorantha) - A review of two closely related medicinal plants from tropical Africa. J Ethnopharmacol. 2015;176:438-62. DOI: 10.1016/j.jep.2015.10.021
Kémeuzé VA, Nkongmeneck BA. Annickia affinis (Exell) Versteegh & Sosef. [Internet] Record from PROTA4U. 2011. Published 2007. Accessed August 3, 2022. Available:http://www.prota4u.org/search.asp
Desjardins RE, Canfield CJ, Haynes JD, Chulay JD. Quantitative Activity Semiautomated Technique. Antimicrob Agents Chemother. 1979;16(6):710-8.
Fekam BF, Madiesse E, Tepongning R, Ngouana V, Mbacham FW, Tsamo E, et al. Antiplasmodial activity of extracts from seven medicinal plants used in malaria treatment in Cameroon. J Ethnopharmacol. 2009;123:483-8. DOI: 10.1016/j.jep.2009.03.008
Kemgne EAM, Mbacham WF, Boyom FF, Zollo PHA, Tsamo E, Rosenthal PJ. In vitro sensitivity of Plasmodium falciparum field isolates to extracts from Cameroonian Annonaceae plants. Parasitol Res. 2012;110(1):109-17. DOI: 10.1007/s00436-011-2456-7
Musuyu MD, Fruth BI, Nzunzu LJ, Kambu OK, Tona GR, Kanyanga CR, et al. In vitro antiprotozoal and cytotoxic activity of 33 ethonopharmacologically selected medicinal plants from Democratic Republic of Congo. J Ethnopharmacol. 2012;141(1): 301-8. DOI: 10.1016/j.jep.2012.02.035
Imieje V, Zaki AA, Fasinu P, Ali Z, Khan AI, Tekwani B, Khan IS, Nosa OE, Falodun A. Antiprotozoal and cytotoxicity studies of fractions and compounds from Enantia chlorantha. Trop J Nat Prod Res. 2017;1(2):89-94. DOI: 10.26538/tjnpr/v1i2.8
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