Ethnobotanical Survey and Some Biological Activities of Ageratum conyzoides Collected in Southern-Benin

Kamirou Chabi-Sika

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin and Laboratory of Biochemistry and Food & Medicinal Formulations, National University of Sciences, Technologies, Engineering and Mathematics of Abomey, Dassa-Zoumè BP 14, Benin.

Haziz Sina *

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Bawa Boya

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Hafiz A. Salami

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Gabin A. Dossou

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Ibrahima Mama-Sirou

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin and Laboratory of Physiopathology, Molecular Pharmacology and Toxicology, 01 BP: 4521 Cotonou, Benin.

Glorieuse Dansou

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Akim Socohou

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Martial Nounagnon

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Halfane Lehmane

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

Adolphe Adjanohoun

National Agronomic Research Institute of Benin, 01 BP 884 Cotonou, Republic of Benin.

Lamine Baba-Moussa

Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou 05 BP 1604, Benin.

*Author to whom correspondence should be addressed.


Abstract

Aims: Ageratum conyzoides L. is a small annual herbaceous highly odorous plant use in traditional medicine. The aim of this study is to evaluate in vitro antioxidant potential, toxicity and antimicrobial activity of aerial part extracts of A. conyzoides on strains potentially involved in vaginal infections.

Methodology: An ethnobotanical survey has been carried out on A. conyzoides among ethnobotanists and traditional therapists in fifteen markets in the communes of Abomey- Calavi, Cotonou, Zogbodomey, Bohicon and Abomey in Southern Benin. The phytochemical screening was a qualitative analysis based on staining and precipitation reactions. Antimicrobial activity of A. conyzoides aqueous and ethanolic extracts was evaluated on reference and clinical strains of Staphylococcus aureus, Candida albicans and Escherichia coli using micro dilutions method in wells from. The toxicity of A. conyzoides extracts was determine using Artemia salina larvae, whereas the antiradical activity was evaluated using the Ferric Reducing Antioxidant Power (FRAP) method.

Results: The survey showed that the population of Southern-Benin uses A. conyzoides according to different modes of preparation. Also, the administration in the treatment of a variety of pathologies affecting the female reproductive system. The phytochemical screening revealed the presence of flavonoids, tannins, anthocyanins, triterpenes and C- heterosides. The yield of 6.18% for the aqueous extract and 4.32% for the ethanolic extract as recorded. The highest inhibition diameter (24.05 ± 0.5 mm) was obtained using aqueous extract against the clinical S. aureus strain. In contrast, the lowest inhibition diameter (10±0 mm) was obtained against the S. aureus ATCC29213 with the same extract. The Minimum Inhibitory Concentration varied from 2.5 to 5 mg/ml. Both extracts show a bactericidal and fungicidal effect on the different strains studied but the sensitivity of the strains to the aqueous extract is better compared to the ethanolic extract. In addition, the aqueous extracts showed higher antioxidant power compared to the ethanolic extract. No toxicity is revealed for both extracts.

Conclusion: The results obtained show that the aqueous and ethanolic extracts of the aerial part of A. conyzoides have antioxidant and antimicrobial properties on strains involved in vaginal infections and do not present a toxicity.

Keywords: Ageratum conyzoides, antioxidant potential, antimicrobial activity, toxicity, Staphylococcus aureus, Candida albicans, Escherichia coli


How to Cite

Chabi-Sika, K., Sina, H., Boya, B., Salami, H. A., A. Dossou, G., Mama-Sirou, I., Dansou, G., Socohou, A., Nounagnon, M., Lehmane, H., Adjanohoun, A., & Baba-Moussa, L. (2023). Ethnobotanical Survey and Some Biological Activities of Ageratum conyzoides Collected in Southern-Benin. International Journal of Biochemistry Research & Review, 32(1), 9–25. https://doi.org/10.9734/ijbcrr/2023/v32i1793

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References

Cuzin L, Delpierre C. Epidemiologie des maladies infectieuses. EMC-Mal Infect. 2005;2(1):157-62.

Holmes KK, Bertozzi S, Bloom BR, et al. Major Infectious Diseases: Key Messages from Disease Control Priorities, Third Edition. In: Holmes KK, Bertozzi S, Bloom BR, et al., editors. Major Infectious Diseases. 3rd edition. Washington (DC): The International Bank for Reconstruction and Development / The World Bank; 2017 Nov 3. Chapter 1.

Available:https://www.ncbi.nlm.nih.gov/books/NBK525197/ doi: 10.1596/978-1-4648-0524-0_ch1

WHO. 2002. Strategy for Traditional Medicine for 2002–2005, OMS/WHO ed. Geneva, 65p. (French)

Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, Armanini D. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol. 2008;140(1):3-11.

DOI:http://doi:10.1016/j.ejogrb.2008.03.009.

Bilardi JE, Walker S, Temple-Smith M, McNair R, Mooney-Somers J, Bellhouse C. The burden of bacterial vaginosis: Women’s experience of the physical, emotional, sexual and social impact of living with recurrent bacterial vaginosis. PLoS One. 2013;8(9):e74378.

Available:http://dx.doi.org/10.1371/journal.pone.0074378.

Willems HME, Ahmed SS, Liu J, Xu Z, Peters BM. Vulvovaginal Candidiasis: A Current Understanding and Burning Questions. J Fungi. 2020;6(1):27.

Available:https://doi.org/10.3390/jof6010027

Palmeira-de-Oliveira R, Palmeira-de-Oliveira A, Martinez-de-Oliveira J. New strategies for local treatment of vaginal infections. Adv. Drug Deliv. Rev. 2015;92:105– 122.

Tonato Bagnan JA, Denakpo JL, Aguida B, Hounkpatin L, Lokossou A, De Souza J, Perrin RX. Epidemiology of the gynecological and mammary cancer to the HOMEL and in the CUGO Cotonou, Benin. Bull Cancer. 2013;100(2):141-146.

WHO (World Health Organization), Antimicrobial Resistance. Regional Office for the Western Pacific, Seventieth Session Manila, Philippines. 2019;8. (French).

Superti F, De Seta F. Warding off recurrent yeast and bacterial vaginal infections: Lactoferrin and Lactobacilli. Microorganisms. 2020;8(1):130.

DOI: 10.3390/microorganisms8010130

Sanogo R, Diallo D, Diarra S, Ekoumou C, Bougoudogo F. Activité antibactérienne et antalgique de deux recettes traditionnelles utilisées dans le traitement des infections urinaires et la cystite au Mali. Mali Méd. 2006; Tome21(1):18.

Dembélé LD, Dramé BSI, Haïdara M, Koné C, Sanogo R. Paramètres physicochi-miques et activité antibactérienne de trois plantes médicinales, utilisées dans la prise en charge des infections urinaires au Mali. J Soc Ouest-Afr Chim. 2022;51:10–16.

Biswas B, Rogers K, Claughin F, Daniels D, Yadav A. Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. Int J Microbiol. 2013;ID 746165:7.

Available:https://doi.org/10.1155/2013/746165

Oullai L, Chamek C. Contribution to the ethnopharmacognosic study of medicinal plants used for the treatment of digestive tract disorders in Kabylie. Dissertation Doctor of Pharmacy, Mouloud Mammeri University, Faculty of Medicine. 2018;199. (French).

WHO/MS-Benin, WHO Country Cooperation Strategy 2009–2013 Benin. WHO Regional Office for Africa, 2009. ISBN: 978 929 031 1249 (NLM Classification: WA 507 540 HB35)

Chabi-Sika K, Sina H, Boya B, Bade F, Hounnou T, Badoussi ME, Adjatin A, Baba- Moussa L. Richardia brasilensis collected in Southern-Benin: Phytochemical, Antimicrobial Activity and Toxicity. Asian J Biol. 2021;13(4):22-23.

Yapi AB, Kassi NJ, Fofie NBY, Zirihi GN. Etude ethnobotanique des Asteraceae médicinales vendues sur les marches du district autonome d’Abidjan (Côte d’Ivoire). Int J Biol Chem Sci. 2015;9(6): 2633-47.

DOI: http://dx.doi.org/10.4314/ijbcs.v9i6.10

Telefo PB, Lemfack MC, Bayala B, Lienou LL, Goka CS, Yemele MD, Mouokeu C, Tagne SR, Moundipa FP. Enquête ethnopharmacologique des plantes utilisées dans le traitement de l’infertilité féminine dans les localités de Fossong-Wentcheng et Foto, Cameroun. Phytothérapie. 2012;10(1):25-34.

Akoegninou A, van der Burg WJ, van der Maesen LlO, Adjakidjè V, Essou JP, Sinsin B, Yédomonhan H. Flore Analytique du Benin. Université d'Abomey-Calavi, Cotonou, République du Bénin. Cotonou & Wageningen. Backhuys Publishers. 2006;1034.

Fanou BA, Klotoe JR, Fah L, Dougnon V, Koudokpon CH, Toko G, Loko F. Ethnobotanical survey on plants used in the treatment of candidiasis in traditional markets of southern Benin, BMC Complementary Med Ther. 2020;20(1):1-18.

Akabassi BS, Djossou JA, Tchobo PF, Tchatcha DA, Houénon GHA, Yovo M, Dédjiho CC, Bogninou-Agbidinoukoun RSG, Soumanou MM. Criblage phytochimique et évaluation des activités antiradicalaire et antimicrobienne des organes du Detarium microcarpum Guill. & Perr de la zone soudanienne du Benin. J Soc Ouest-Afr Chim. 2021;050:68- 75

Adjou ES, Aoumanou MM. Efficacité des extraits de plantes dans la lutte contre les moisissures toxinogènes isolées de l’arachide en post-récolte au Bénin. J Appl Biosci. 2013;70:5555-66.

Roko OG, Dougnon V, Hounkpatin A, Klotoé JR, Baba-Moussa L. Anti-inflammatory, Analgesic and Antipyretic Properties of Ethanolics Extrats of Three Plants of Beninese’s Pharmacopoeia: Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia. Asian J Biol. 2019;8(4):1-8.

Houghton P, Raman J. A Laboratory handbook for the fractionation of natural extracts. Chapman et Hall, London. 1998;199.

Sina H, N’tcha C, Dah-Nouvlessounon D, Gnama-Tchao G, Boya B, Socohou A, Sanni ARA, Baba-Moussa F, Adjanohoun A, Baba-Moussa L. Molecular characterization of high-risk infection vaginal bacteria isolated from pregnant women in CHU-MEL of Cotonou (Benin). Afr J Microbiol Res. 2021;15(12):592-604.

Amoussa AMO, Sanni A and Lagnika L. Antioxidant activity and the estimation of total phenolic, flavonoid and flavonol contents of the bark extracts of Acacia ataxacantha. J Pharmacogn Phytochem. 2015;4(2):172-178.

Kamanzi AK. Medicinal plants of Côte d'Ivoire: phytochemical investigations guided by biological tests. Doctoral thesis, University of Cocody, Abidjan, Ivory Coast. 2002;176.

Kawsar SMA, Huq E, Nahar N. Cytotoxicity assessment of the aerial parts of Macrotyloma uniflorum linn. Intl J Pharmacol. 2008;4(4):297-300.

Dieng SIM, Fall AD, Diatta-Badji K, Sarr A, Sene M, Sene M, Mbaye A, Diatta W, Bassene E. Evaluation de l’activité antioxydante des extraits hydro-ethanoliques des feuilles et écorces de Piliostigma thonningii Schumach. Int J Biol Chem Sci. 2017;11(2):768-776.

Mousseux M. Toxicity test on Artemia salina larvae and maintenance of a farm and blanes, second year internship report. DEUST Aquaculture; University Center of New Caledonia, France, 1995; (French)

Mangambu M, Mushagalusa K, Kadima N. Contribution à l’étude phytochimique de quelques plantes médicinales antidiabétiques de la ville de Bukavu et ses environs (Sud-Kivu, R.D. Congo). J Appl Biosci. 2014;75:6211-20.

Available:http://dx.doi.org/10.4314/jab.v75i1

Ouattara D. Contribution to the inventory of significant medicinal plants used in the Divo region (southern forest of the Ivory Coast) and to the diagnosis of the Guinea pepper plant: Xylopia aethiopica (Dunal) A Rich (Annonaceae). Doctoral thesis University of Cocody, Abidjan (Ivory Coast), UFR Biosciences. 2006;184. (French)

N'Guessan K. Medicinal plants and traditional medical practices among the Abbey and Krobou peoples of the Department of Agboville (Ivory Coast). State Doctorate Thesis, University of Cocody-Abidjan, U.F.R. Biosciences. 2008;235. (French)

Ndacnou MK, Pantaleon A, Tchinda JS, Mangapche ELN, Keumedjio F, Boyoguemo DB. Phytochemical study and anti-oomycete activity of Ageratum conyzoides Linnaeus. Industrial Crop Products. 2020;153:112589.

Available:https://doi.org/10.1016/j.indcrop.2020.112589

Kasali AA, Winterhalter P, Adio AM, Knapp H, Bonnlander B. Chromenesin Ageratum conyzoides L. Flavour Fragr J. 2002;17:247–50.

Usman LA, Zubair MF, Olawore NO, Muhammad NO, M’Civer FA, Ismaeel RO. Chemical constituents of flower essential oil of Ageratum conyzoides growing in Nigeria. Elixir Org Chem. 2013;54:12463–5.

Okereke SC, Chukwudoruo CS, Nwaokezie CO. Phytochemical screening using GC-FID and sub-chronic assessment of Hydroethanolic leaf extract of Ageratum conyzoides Linn. On albino rats. J Med Plants Stud. 2017;5:282–7.

Folashade KO, Omoregie EH, Ochogu AP. Standardization of herbal medicines–A Review. Int J Biodiver Conserv. 2012; 4(3):101-112

Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complementary Altern Med. 2005;5(1):1-7.

Wuyep PA, Musa HD, Ezemokwe GC, Nyam DD, SilaGyang MD. Phytochemicals from Ageratum conyzoides L. Extracts and their Antifungal Activity against Virulent Aspergillus spp. J Academia Industrial Res. 2017;6(3):32-39.

Omole OA, Oladipo JO, Orimolade BO, Ajetomobi OO, Olorumaiye KS, Dosumu OO. Anti-Oxidant and Anti-Microbial Activities of the Root and Leaf Extracts of Ageratum conyzoides L. Agriculture Conspectus Scientificus. 2019;84(3):295-304.

Chahal R, Nanda A, Akkol EK, Sobarzo-Sánchez E, Arya A, Kaushik D, Dutt R, Bhardwaj R, Rahman MH, Mittal V. Ageratum conyzoides L. and Its Secondary Metabolites in the Management of Different Fungal Pathogens. Molecules. 2021; 26, 2933.

Available: https://doi.org/10.3390/.

Yessoufou A, Gbenou J, Grissa O, Hichami A, Simonin A-M, Tabka Z, Moudachirou M, Moutairou K, Khan NA. Anti-hyperglycemic effects of three medicinal plants in diabetic pregnancy: modulation of T cell proliferation. BMC Complementary Altern Med. 2013;13(77) :13.

Connolly JD, Hill RA. Triterpenoids. Natural Product Reports. 2007;24:465-486.

Odeleye OP, Oluyege JO, Aregbesola OA, Odeleye PO. Evaluation of preliminary phytochemical and antibacterial activity of Ageratum conyzoides (L.) on some clinical bacterial isolates. Int J Eng Sci. 201;3:1–5.

Ouattara KE, Doga D, Zirihi GN. Evaluation In vitro du Pouvoir Fongicide des Extraits De Erigeron floribundus (Kunth.) Sch. Bip. (Asteraceae) sur Sclerotium rolfsii et Colletotrichum musae Deux Champignons Phytopathogènes. Eur Sci J. 2019;15(9):370.

Available:https://doi.org/10.19044/esj.2019.v15n9p370

Acheampong F, Larbie C, Arthur FKN, Appiah-Opong R, Tuffour I. Antioxidant and anticancer study of Ageratum conyzoides aqueous extracts. J Global Biosci. 2015;4(1):1804-15.

Bédié AP, N’guessan BB, Yapo AF, N’guessan JD, Djaman JA. Activités antioxydantes de dix plantes médicinales de la pharmacopée ivoirienne. Sci Nat. 2011;8(1):1 -11

Houngbèmè AG, Gandonou C, Yehouenou B, Kpoviessi SDS, Sohounhloue D, Moudachirou M, Gbaguidi FA. Phytochemical analysis, toxicity and antibacterial activity of Benin medicinal plants extracts used in the treatment of sexually transmitted infections associated with HIV/AIDS. Int J Pharmaceutical Sci Res. 2014;1739-1745.

Djeneb C, Basile YA, Yvette BNF, Etienne OK, Noël ZG. Etude Comparative des Toxicités Cellulaires et Aigües de Ageratum conyzoides L. et de Acanthospermum hispidum DC. Eur Sci J. 2021;17(40):74.

Available: https://doi.org/10.19044/esj 2021.v17n40p74