Mineral Element Contents and Antisickling Activity of Cyttaranthus congolensis and Hypoxis angustifolia Traditionally Used to Treat Sickle Cell Disease in Kwilu Province, Democratic Republic of the Congo
International Journal of Biochemistry Research & Review,
Page 81-90
DOI:
10.9734/ijbcrr/2022/v31i10790
Abstract
The objective of the present study was to determine the composition in mineral elements, the antisickling activity and the osmotic brittleness test, of the twenty three extracts of two plants Cyttaranthus congolensis, Hypoxis angustifolia used in the management of sickle cell disease by the traditional healers in Kwilu province. Mineral composition analyses of these two plants were performed using X-ray fluorescence spectrometric method. Twenty three( 23) mineral elements were identified in each of these two plants among others: Potassium (K), Phosphorus (P), Calcium (Ca), Sodium (Na), Magnesium (Mg), Sulfur (S), Chlorine (Cl) and trace elements such as: Aluminum (Al), Silicon (Si), Vanadium (V), Chromium (Cr), Manganese (Mn), Iron (Fe), Nickel (Ni), Copper (Cu), Zinc (Zn), Selenium (Se), Bromine (Br), Molybdenum (Mo), Tin (Sn), Iodine (I), Barium (Ba) and Lead (Pb). Mineral elements related to sickle cell disease are: Iron (Fe), Zinc (Zn), Selenium (Se), Copper (Cu), Calcium (Ca), Magnesium (Mg) and Manganese (Mn). Of all these elements, Potassium and Calcium are in higher content while Lead, Tin, Bromine, Copper and Nickel are in trace amounts. The antisickling activity of Cyttaranthus congolensis and Hypoxis angustifolia extracts, was tested using the Emmel test. The results obtained showed a significant in vitro antisickling activity for both plants. The osmotic fragility test used to confirm the Emmel test showed that the hemolysis rate decreased with increasing NaCl concentration. In the presence of anthocyanins, the antihemolytic activity at different NaCl concentrations was higher than the control. This indicates the action of the extracts of these plants on the osmotic fragility of red blood cell membrane.
Keywords:
- Cyttaranthus congolensis
- Hypoxis angustifolia
- osmotic fragility
- sickle cell disease
- hemolysis
- mineral elements
- fluorescence
How to Cite
Kitadi, J. M., Inkoto, C. L., Tshibangu, S.-T., Tshilanda, D. D., Kalulu, T., Kawata, P., Ngbolua, K.-T.-N., & Mpiana, P. T. (2022). Mineral Element Contents and Antisickling Activity of Cyttaranthus congolensis and Hypoxis angustifolia Traditionally Used to Treat Sickle Cell Disease in Kwilu Province, Democratic Republic of the Congo. International Journal of Biochemistry Research & Review, 31(10), 81-90. https://doi.org/10.9734/ijbcrr/2022/v31i10790
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Inkoto CL, Ngbolua KN, Bokungu PE, Masengo CA, Iteku JB, Tshilanda DD, Tshibangu DS-T and Mpiana PT. Phytochemistry and Pharmacology of Aframomum angustifolium (Sonn.) K. Schum (Zingiberaceae): A Mini Review. SARJNP. 2021;5(1):21-36.
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Kitadi MJ, Mazasa PP, Tshibangu DST, Memvanga PB, Ngbolua KN, Taba NK, Noki PV, Mpiana PT. Anti-sickling and antioxidant activities of anthocyanins extracts from Dissotis brazzae Cogn (Melastomataceae). Journal of Advancement in Medical and Life Sciences; 2015.
Khan AR, Awan FR. Metals in the pathogenesis of type 2 diabetes. J Diabetes Metab Disord. 2014;13(1):16.
DOI: 10.1186/2251-6581-13-16
PMID: 24401367; PMCID: PMC3916582
Kitadi MJ. Détermination de la teneur en éléments minéraux et de l’activité antidrépanocytaire des plantes médicinales utilisées dans la Province de Kwilu (R.D. Congo). Thèse de doctorat, Département de chimie, Faculté des Sciences, Unikin., Inedit. 2021;187.
Tshilanda DD, Onyamboko DVN, Babady PB, Mutwale PK, Tsalu PV, Tshibangu DST, Ngombe NK., Ngbolua KN, Mpiana PT. Chemical fingerprint and anti-sickling activity of Rosmarinic acid and Methanolic extracts from three Species of Ocimum from DR Congo. Journal of Biosciences and Medicines. 2016;4:59-68.
Kitadi MJ, Pambi MP, Tshibangu DST, Mushagalusa KF, Tshilanda DD, Ngbolua KN, Mpiana PT. Ethnopharmacological Survey and Antisickling Activity of Plants Used in the Management of Sickle Cell Disease in Kikwit City, DR Congo. Hindawi Evidence-Based Complementary and Alternative Medicine. 2020;10.
Mpiana PT, Tshibangu DST, Shehonde OM, Ngbolua KN. In-vitro antidrepanocytary activity (antisickle cell anaemia) of some Congolese plants. Phytomedicine. 2007;14(2-3):192-195.
Mpiana PT, Makelele LK, Oleko RW, Bokota MT, Tshibangu DST, Ngbolua K.N, Mbala MB, Atibu EK, Nsimba SM. Antisickling activity of medicinal plants used in the management of Sickle Cell Disease in Tshopo district, D.R. Congo, Australian Journal of Medical Herbalism. 2010;22(4):132-137.
Ngbolua KN, Mpiana PT, Tshibangu DST, Mazasa PP, Gbolo ZB, Atibu EK, Kadima JN, Kasali FM. In vitro anti-sickling and radical scavenging activities of a poly-herbal formula (Drepanoalpha®) in Sickle cell erythrocyte and acute toxicity study in Wistar albino rats. European Journal of Medicinal Plants. 2014;4(10):1251-1267.
Kitadi JM, Inkoto CL, Lengbiye EM, Tshibangu DS-T, Tshilanda DD, Ngbolua KN, Taba KM, Mbala BM, Schmitz B, Mpiana PT. Antisickling activity and mineral content of Hura crepitans l., Alternanthera bettzickiana (regle) g. Nicholson and Dissotis brazzae cogn, plants used in the management of sickle cell disease in kwilu province, democratic republic of the Congo. ejpmr. 2019;6 (12):79-83
Mpiana PT, Balangayi EK, Kanangila AB, Kalonda EM, Ngbolua KN, Tshibangu DST, Atibu EK, Lumbu JBS. Activité antidrépanocytaire et thermodégradation des anthocyanes extraits de Sterculia quinqueloba et Ficus capensis,Int. J. Biol. & Chem. Sci. 2009;3(3):551-560.
Tshibangu DS. Phytochemical and anti-drepanocytosis studies of Cajanus cajan, Callistemon viminalis, Melaleuca bracteata Var. revolution gold and Syzygium guineensis. Master thesis, Faculty of science and Agriculture, University of KwaZulu Natal, Republic of South Africa; 2010.
Ebenyi LN, Uraku AJ. Phytochemical and nutritional evaluation of aqueous and ethyl acetate leaf extracts of Alchornea cordifolia. Research Journal of Pharmacology and Pharmacy. 2017;1(5): 2572-8377.
Yoka J, Loumeto JJ, Gaudentius JD, Akouango P, Epron D. Evaluation des teneurs en éléments minéraux des feuilles de Niébé cultivés dans la zone Boundji, République du Congo. Journal of Applied Biosciences. 2014;79:6799-6807.
Alikwe CN, Owen OJ. Evaluation of the chemical and phytochemical constituents of Alchornea cordifolia leaf meal as potential feed for monogastric livestock. 2014;2(3):360-368.
Yisa J, Egila JN, Darlinton AO. Chemical composition of Annona senegalensis from Nupe land, Nigeria. African Journal of Biotechnology. 2010;9(26):4106-4109.
Elenga M, Massamba J, Kobawila SC, Makosso VG et Silou T. Evaluation et amélioration de la qualité nutritionnelle des pâtes et des bouillies de maïs fermenté au Congo. Int. J. Biol. Chem. Sci. 2009;3(6):1274-1285.
Mart´inez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-L´opez C, Bast´ias E, Garc´ia-Viguera C, Carvajal Minerals in plant food: effect of agricultural practices and role in human health. A review Agron. Sustain. Dev, 2010;30:295–309
DOI: 10.1051/agro/2009022
Masengo CA, Ngbolua KN, Gbolo BZ, Inkoto CL, Robijaona B, Iteku JB, Ilumbe GB, Mpiana PT. Drépanocytose: Approche Bioclinique, Cibles Biologiques d’Intérêt Thérapeutique et Perspectives. International Journal of Progressive Sciences and Technologies (IJPSAT). 2021;28(2):539-545.
Hanan ZA, Ibrahim NH, Younis FE, Shaker YM, Donia GR. Scrutinizing of trace elements and antioxidant enzymes changes in Barki Ewes Fed Salt-tolerant plants under South Sinai conditions. Journal of American Science. 2014;10 (2):54-62
Panzu NN, Inkoto CL, Ngbolua KN, Mukeba FB, Kitadi JM, Taba K, Mbala BM, Tshilanda DD, Kayembe J-PK, Mpiana PT. Review on the phytochemistry, toxicology and bioactivities of Euphorbia hirta L.: A potential antisickling medicinal plant species. JMPHTR. 2020;7:8-18.
Inkoto CL, Kayembe J-PK, Mpiana PT, Ngbolua KN. A review on the Phytochemistry and Pharmacological properties of Picralima nitida Durand and H. (Apocynaceae family): A potential anti Covid-19 medicinal plant species. Emer Life Sci Res. 2020;6(1):64-75.
Iteku JB, Bongo GN, Mbiya JN, Wambale JM, Mutwale PK, Inkoto CL and Ngbolua KN. Phytochemical Analysis and Evaluation of Bioactivities of Cola acuminata Extracts. Journal of Biomedical and Life Sciences. 2021;1(1): 51-65.
Inkoto CL, Masengo CA, Falanga CM, Membo BW-M, Amogu J-JD, Mahendra ISM, Kayembe J-PK., Mpiana PT, Ngbolua K.N. A Mini-Review on the Phytochemistry and Pharmacology of the Plant Carica Papaya L. (Caricaceae). Britain International of Exact Sciences (BIoEx) Journal. 2020;(3):663-675.
Inkoto LC, Bongo NG, Kapepula MP, Masengo AC, Gbolo ZB, Tshiama C et al. Microscopic features and chromatographic fingerprints of selected congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Emergent Life Sciences Research. 2018;4(1):1-10.
Ngbolua KN, Rafatro H, Rakotoarimanana H, Ratsimamanga US, Mudogo V, Mpiana PT, Tshibangu DST. Pharmacological screening of some traditionally-used antimalarial plants from the Democratic Republic of Congo compared to its ecological taxonomic equivalence in Madagascar, Int. J. Biol. Chem. Sci. 2011; 5(5):1997-1804.
Ngbolua KN, Hajatiana R, Herintsoa R, Suzanne U-R, Mudogo V, Mpiana PT, Tshibangu DST. Comparative antimalarial and cytotoxic activities of two Vernonia species: V. amygdalina from the Democratic Republic of Congo and V. cinerea subsp vialis endemic to Madagascar, Int. J. Biol. Chem. Sci. 2011; 5(1):345-353.
Mbadiko CM, Inkoto CL, Gbolo BZ, Lengbiye EM, Kilembe JT, Matondo A, Mwanangombo DT, Ngoyi EM, Bongo GN, Falanga CM, Tshibangu DS-T, Tshilanda DD, Ngbolua KN and Mpiana PT. A Mini Review on the Phytochemistry, Toxicology and Antiviral Activity of Some Medically Interesting Zingiberaceae Species, JOCAMR. 2020;9(4):44-56.
Kitadi MJ, Inkoto CL, Lengbiye EM, Tdhibangu DST, Tshilanda DD, Ngbolua KN, Taba M, Mbala BM, Schmitz B, Mpiana PT. Mineral composition of medicinal plants traditionally used in the management of sickle cell disease in the Kwilu province (Congo- Kinshasa). Sumerianz Journal of Biotechnology. 2021;(4):16-21.
Inkoto CL, Ngbolua KN, Bokungu PE, Masengo CA, Iteku JB, Tshilanda DD, Tshibangu DS-T and Mpiana PT. Phytochemistry and Pharmacology of Aframomum angustifolium (Sonn.) K. Schum (Zingiberaceae): A Mini Review. SARJNP. 2021;5(1):21-36.
Inkoto CL, Ngbolua KN, Kilembe JT, Masengo CA, Lukoki FL, Tshilanda DD, Tshibangu DS-T, Mpiana PT. A Mini Review on the Phytochemistry and Pharmacology of Aframomum alboviolaceum (Zingiberaceae). South Asian Research Journal of Natural Products. 2021;4(3): 24-35.
Mpiana PT, Bokota, MT, Ndjele MBL, Mudogo V, Tshibangu DST, Ngbolua KN, Atibu EK, Kwembe JTK and Makelele LK. Antisickling activity of three species of justicia from Kisangani (DR Congo). J. tenella, J. gendarussa and J.insularis, Int. J. Biol. Chem. Sci. 2010;4(6):1953-1961.
Bongo G, Inkoto C, Masengo C, Tshiama C, Lengbiye E, Djolu R. and Kapepula M et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine. 2017;2:52-59.
Kitadi MJ, Mazasa PP, Tshibangu DST, Memvanga PB, Ngbolua KN, Taba NK, Noki PV, Mpiana PT. Anti-sickling and antioxidant activities of anthocyanins extracts from Dissotis brazzae Cogn (Melastomataceae). Journal of Advancement in Medical and Life Sciences; 2015.
Khan AR, Awan FR. Metals in the pathogenesis of type 2 diabetes. J Diabetes Metab Disord. 2014;13(1):16.
DOI: 10.1186/2251-6581-13-16
PMID: 24401367; PMCID: PMC3916582
Kitadi MJ. Détermination de la teneur en éléments minéraux et de l’activité antidrépanocytaire des plantes médicinales utilisées dans la Province de Kwilu (R.D. Congo). Thèse de doctorat, Département de chimie, Faculté des Sciences, Unikin., Inedit. 2021;187.
Tshilanda DD, Onyamboko DVN, Babady PB, Mutwale PK, Tsalu PV, Tshibangu DST, Ngombe NK., Ngbolua KN, Mpiana PT. Chemical fingerprint and anti-sickling activity of Rosmarinic acid and Methanolic extracts from three Species of Ocimum from DR Congo. Journal of Biosciences and Medicines. 2016;4:59-68.
Kitadi MJ, Pambi MP, Tshibangu DST, Mushagalusa KF, Tshilanda DD, Ngbolua KN, Mpiana PT. Ethnopharmacological Survey and Antisickling Activity of Plants Used in the Management of Sickle Cell Disease in Kikwit City, DR Congo. Hindawi Evidence-Based Complementary and Alternative Medicine. 2020;10.
Mpiana PT, Tshibangu DST, Shehonde OM, Ngbolua KN. In-vitro antidrepanocytary activity (antisickle cell anaemia) of some Congolese plants. Phytomedicine. 2007;14(2-3):192-195.
Mpiana PT, Makelele LK, Oleko RW, Bokota MT, Tshibangu DST, Ngbolua K.N, Mbala MB, Atibu EK, Nsimba SM. Antisickling activity of medicinal plants used in the management of Sickle Cell Disease in Tshopo district, D.R. Congo, Australian Journal of Medical Herbalism. 2010;22(4):132-137.
Ngbolua KN, Mpiana PT, Tshibangu DST, Mazasa PP, Gbolo ZB, Atibu EK, Kadima JN, Kasali FM. In vitro anti-sickling and radical scavenging activities of a poly-herbal formula (Drepanoalpha®) in Sickle cell erythrocyte and acute toxicity study in Wistar albino rats. European Journal of Medicinal Plants. 2014;4(10):1251-1267.
Kitadi JM, Inkoto CL, Lengbiye EM, Tshibangu DS-T, Tshilanda DD, Ngbolua KN, Taba KM, Mbala BM, Schmitz B, Mpiana PT. Antisickling activity and mineral content of Hura crepitans l., Alternanthera bettzickiana (regle) g. Nicholson and Dissotis brazzae cogn, plants used in the management of sickle cell disease in kwilu province, democratic republic of the Congo. ejpmr. 2019;6 (12):79-83
Mpiana PT, Balangayi EK, Kanangila AB, Kalonda EM, Ngbolua KN, Tshibangu DST, Atibu EK, Lumbu JBS. Activité antidrépanocytaire et thermodégradation des anthocyanes extraits de Sterculia quinqueloba et Ficus capensis,Int. J. Biol. & Chem. Sci. 2009;3(3):551-560.
Tshibangu DS. Phytochemical and anti-drepanocytosis studies of Cajanus cajan, Callistemon viminalis, Melaleuca bracteata Var. revolution gold and Syzygium guineensis. Master thesis, Faculty of science and Agriculture, University of KwaZulu Natal, Republic of South Africa; 2010.
Ebenyi LN, Uraku AJ. Phytochemical and nutritional evaluation of aqueous and ethyl acetate leaf extracts of Alchornea cordifolia. Research Journal of Pharmacology and Pharmacy. 2017;1(5): 2572-8377.
Yoka J, Loumeto JJ, Gaudentius JD, Akouango P, Epron D. Evaluation des teneurs en éléments minéraux des feuilles de Niébé cultivés dans la zone Boundji, République du Congo. Journal of Applied Biosciences. 2014;79:6799-6807.
Alikwe CN, Owen OJ. Evaluation of the chemical and phytochemical constituents of Alchornea cordifolia leaf meal as potential feed for monogastric livestock. 2014;2(3):360-368.
Yisa J, Egila JN, Darlinton AO. Chemical composition of Annona senegalensis from Nupe land, Nigeria. African Journal of Biotechnology. 2010;9(26):4106-4109.
Elenga M, Massamba J, Kobawila SC, Makosso VG et Silou T. Evaluation et amélioration de la qualité nutritionnelle des pâtes et des bouillies de maïs fermenté au Congo. Int. J. Biol. Chem. Sci. 2009;3(6):1274-1285.
Mart´inez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-L´opez C, Bast´ias E, Garc´ia-Viguera C, Carvajal Minerals in plant food: effect of agricultural practices and role in human health. A review Agron. Sustain. Dev, 2010;30:295–309
DOI: 10.1051/agro/2009022
Masengo CA, Ngbolua KN, Gbolo BZ, Inkoto CL, Robijaona B, Iteku JB, Ilumbe GB, Mpiana PT. Drépanocytose: Approche Bioclinique, Cibles Biologiques d’Intérêt Thérapeutique et Perspectives. International Journal of Progressive Sciences and Technologies (IJPSAT). 2021;28(2):539-545.
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