Phytochemical Composition and Antibacterial Activity of a Phytoremedy Used in Traditional Medecine in Côte d’Ivoire
Roger Guy Noel Pehe *
Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA / Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Christelle N’GAMAN-KOUASSI
Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA / Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Kouassi Pierre Alain KONAN
Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA / Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Olivier IRIE Bi Tra
Laboratoire de Biologie et Santé, Spécialité Physiologie Animale, Phytothérapie et Pharmacologie, UFR Biosciences / Université Félix Houphouët-Boigny, 01 BPV 34 Abidjan 01, Côte d’Ivoire.
Janat Akhanovna MAMYRBEKOVA-BÉKRO
Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA / Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Yves-Alain BÉKRO
Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA / Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
This study was conducted on a phytoremedy used in traditional medicine for the treatment of childhood leukemia in Côte d’Ivoire. It provides a scientific basis for its phytochemical composition and antibacterial activity with the aim of enhancing its value.
To achieve this, qualitative tests (mineral composition, detection tests, TLC), quantitative analyses (quantification of phenolic compounds), antioxidant activity (DPPH, FRAP), and antibacterial assays (liquid dilution method) were performed.
Qualitative analysis revealed the presence of phenolic compounds (flavonoids, tannins, and coumarins), alkaloids, sterols and polyterpenes, saponins, proteins, and oligosaccharides. The mineral composition analysis showed 17 elements, four of which were present in significant concentrations: calcium (Ca), potassium (K), magnesium (Mg), and silicon (Si). Quantification revealed notable and variable concentrations of phytoconstituents in the decoction (total phenolics: 347.150 ± 3.801 mg GAE/g DM; total flavonoids: 115.068 ± 7.527 mg QE/g DM; condensed tannins: 46.343 ± 4.644 mg CE/g DM; hydrolysable tannins: 138.806 ± 15.217 mg GAE/g DM and total coumarins: 49.094 ± 1.907 mg CE/g DM) compared to the macerate and infusion. Furthermore, the extracts demonstrated good antiradical activity in both DPPH and FRAP assays. The antibacterial activity also showed an effect of the extracts on Escherichia coli and Staphylococcus aureus.
The results of this study could provide scientific support for the pharmacological activity attributed to this phytoremedy.
Keywords: Phytochemical composition, phytoremedy, traditional medicine, antiradical activity, antibacterial activity