International Journal of Biochemistry Research & Review,
Aims: The study is aimed to isolate of polyprenols from grape Vitis vinifera L. leaves by different methods and to determine of polyprenol content of plant leaves growing in various regions of Uzbekistan.
Study Design: Isolation of polyprenols.
Place and Duration of Study: Department of Organic Chemistry, Institute of the Chemistry of Plant Substances (ICPS). The study was carried out between January, 2011 and December, 2012.
Methodology: We have studied isolation of polyprenols of grape leaves by using of simple, microwave and ultra sound extraction. Spectral, HPLC and HPTLC methods have used.
Results: It was determined that leaves polyprenols are in type polyprenyl homologues with 10-13 isoprene units where undecaprenol and dodecaprenol were dominant. Extraction carried out of grape leaves in various conditions (usual, microwave, ultrasound). Ethanol was high effective for microwave and ultrasound extraction. It was observed forming of polyprenol and high aliphatic alcohols esters (probably proceeding particularly esterification of polyprenols and aliphatic alcohols by organic acids which contain in extracts).
Conclusion: It was determined that extraction of grape leaves by alcohol in microwave and ultra sound stirring are high effective methods for isolation of polyprenols.