Human Tumor Necrosis Factor-α as a Diagnostic Marker for Chronic Diabetic Nephropathy
Khalid Ibrahem Husein Al Ghodran *
Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Amina Ali Dessouki
Pathology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Heba M. A. Abdelrazek
Physiology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Dahlia Ibrahim Badran
Medical Biochemistry and Molecular Biology Department, Medicine Faculty, Suez Canal University, Ismailia, Egypt and Medical Biochemistry and Molecular Biology Department, Medicine Faculty, Badr University, Cairo, Egypt.
Emad M. Gad
Organic Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: diabetic nephropathy (DN) is a chronic main microvascular consequence of untreated hyperglycemia, affects a significant portion of the population. It's thought to be the main factor causing end-stage kidney disease. TNF-α is involved in the development and course of disease in DN.
Aim: This study aimed to evaluate plasma TNF-α as a new diagnostic marker for early DN.
Patients and Methods: This study included 125 Egyptian subjects attending the out Patients Clinic of the Department of Internal Medicine, 10Th of Ramadan city Health Insurance Hospital and divided as follow:- Control group , Study groups( patient with diabetic mellitus (DM),DN, DN and other complications).
Control& study group were subjected to measurement of glycosylated hemoglobin (HbA1C), creatinine, total cholesterol, triglycerides, F.B.G, GFR ,HDL, LDL, ALT, AST, total protein, albumin, globulin, A/G ratio, HDL, LDL, ACR,TNF-α.
Results: results showed that plasma TNF-α was positive and significantly correlated with BMI, duration of DM, F.B.G, HbAIC, CR, GFR, CHOL, HDL, LDL, AST, A/G ratio, Meanwhile, plasma TNF-α was negatively correlated with ACR, TG, ALT, ALB, TP and GLB.
At cut-off level ≥83.5, TNF-α had 96.7% sensitivity and 79.7% specificity for diagnosing diabetic nephropathy.
Conclusion: The study found that patients in study group had considerably higher plasma levels of TNF-α. This suggests that TNF-α May be used as a markers for predicting chronic DN in patients with type 2 diabetes.
Keywords: Chronic diabetic nephropathy, diagnostic marker, TNF -α, DN, diabetic complications