Effect of Cucumber (Cucumis sativus) Fruit Homogenate on Hypotonicity– induced Haemolysis of Red Blood Cell

Uzuazokaro Mark-Maria Agatemor *

Research and Development, First Focus Research, Nottingham, United Kingdom.

Okwesili Fred Chiligue Nwodo

Department of Biochemistry, University of Nigeria, Nsukka, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Background: Several findings and reports show that people with high intake of Cucumis sativus (Cucumber) have relief in pains, swelling and other inflammatory signs. Human red blood cell membrane stabilization has been used as a method to investigate the mechanism of action of anti-inflammatory drugs. The anti-inflammatory activity of cucumber has been demonstrated in previous study. In this study, we aimed at assessing the effect of cucumber (Cucumis sativus) fruit homogenate on hypotonicity – induced haemolysis of red blood cell.

Methods: Whole fresh blood (3 ml) was collected from healthy volunteer into plastic tubes containing 0.1 volume of 3.8% trisodium citrate and used within 8 hr. The blood sample was centrifuged at 3000 x g for 10 min and the supernatant (plasma) discarded. The pellet was washed twice by resuspending in a volume of normal saline equal to the volume of the supernatant (plasma) and centrifuged at 3000 x g for 10 min. The pellet (0.1 ml) was resuspended in 2.5 ml of normal saline and used as the red blood cell (RBC).

Results: The results revealed that cucumber (Cucumis sativus) fruit homogenate significantly (p ˂ 0.05) inhibited hypotonicity-induced red blood cell haemolysis when compared to indomethacin (a known standard drug).

Conclusion: Cucumber has membrane stabilization effect on the red blood cell.

Keywords: Hypotonicity, membrane stabilization, red blood cells, inflammation, homogenate, Cucumis sativus


How to Cite

Agatemor, Uzuazokaro Mark-Maria, and Okwesili Fred Chiligue Nwodo. 2024. “Effect of Cucumber (Cucumis Sativus) Fruit Homogenate on Hypotonicity– Induced Haemolysis of Red Blood Cell”. International Journal of Biochemistry Research & Review 33 (6):11-16. https://doi.org/10.9734/ijbcrr/2024/v33i6884.

Downloads

Download data is not yet available.

References

Kumar P, Chaudhary N, Sharma NK, Maurya P.K. Detection of oxidative stress biomarkers in myricetin treated red blood cells. RSC Advances. 2016;6(102): 100028–100034.

Anosike CA, Onyechi Obidoa O, Ezeanyika LUS. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum) Journal of Pharmaceutical Sciences. 2012;20(1): 76–81.

Amin C, Abdullah A, Shadman R, Shofiul A, Kishower SA. Human red blood cell membrane stability testing for the estimation of anti-inflammatory activity of methanolic Extract of Millettia Pachycarpa Benth Leaves. International Journal of Pharmaceutical Sciences and Research. 2013;4(12):4587-4590

Sandhya S, Karunakar K, Ragavendhra P. HRBC Membrane Stabilization as a study tool to explore the Anti-Inflammatory activity of Allium cepa Linn.–Relevance for 3R. Journal of Advanced Medical and Dental Sciences Research. 2018;6(6):30-34.

Furst DE, Munster T. Non-steroidal anti-inflammatory drugs, disease-modifying anti-heumatic drugs, non-opoid analgesic and drugs used in gout. In: (B.G. Kartzung ed). Basic and Clinical Pharmacology 8th edn. Lange medical Books/McGraw-Hill, New York. 2001:596-623.

Mariappan G Saha BP, Sutharson L, Singh A, Garg S. Pandey L, Kumara D. Analgesic, anti-inflammatory, antipyretic and toxicological evaluation of some newer 3-methyl pyrazolone derivatives. Saudi Pharm J. 2011;19(2):115–122.

Sharma VK, Mamontov E, Tyagi M. Effects of NSAIDs on the nanoscopic dynamics of lipid membrane. Biochimica et Biophysica Acta (BBA) – Biomembranes. 2020;1862 (2):183100

Philip S, Chinedu I, Olawale O, Ismail MZ, Magaji AP. Effects of Ethanolic Extracts of Fruits of Dennettia tripetala on Kidney Function of Male Albino Rats. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2023;14(1):31–39. Available:https://doi.org/10.9734/ajbgmb/2023/v14i1306

Banerjee A, Manasa S, Ranganna G, Chowdhury S, Singh A, Ravindra NR, Raizada O, Chawl R. Unveiling the Rich Tapestry of Minor Fruit Crops: Cultivation Practices, Market Strategies, and Contributions to Agricultural Diversity and Sustainability. Journal of Advances in Biology & Biotechnology. 2024;27(5):821–834.

Available:https://doi.org/10.9734/jabb/2024/v27i5844

Iwueke AV, Nwodo OFC, Okoli GO. Evaluation of the anti-inflammatory and analgesic activities of Vitex doniana leaves. African Journal of Biotechnology. 2006;5(20):1929-1935.

Sebastian H, Richard JA, Markus R, Laura H, Alexander D, Jose MM, Chris PV, Dawn MEB, Lars K, Christian W, Maikel CR. The Molecular Structure of Human Red Blood Cell Membranes from Highly Oriented, Solid Supported Multi- Lamellar Membranes, Scientific Report. 2017;7: 39661

Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in-vivo and in cell culture: How should you do it and what do the results mean? British Journal of Pharmacology. 2004;142(2): 231-225.

Lobo V, Patil A, Phatak AN, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118–126.

Alugoju Phaniendra, Dinesh Babu Jestadi, and Latha Periyasam. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015;30(1):11–26.

Pattison DJ, Silman AJ, Goodson NJ, Lunt M, Bunn D, Weich A, Bingham S, Khau KT, Day N, Symmons DPM. Vitamin C and the risk of developing inflammatory polyarthritis: Prospective case-control study. Annals of the Rheumatic Diseases. 2004;63:843-847.

Shinde UA, Phadke AS, Nair AM., Mungantiwan AA, Dikshirt VJ, Saref VO. Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia.1999;70:251-257.

Chomicki, G Schaefer H, Renner SS. Origin and domestication of Cucurbitaceae crops: Insights from phylogenies, genomics and archaeology. New Phytologist. 2020:1240–1255.

Wang YH, Joobeur T, Dean RA, Staub JE. Cucurbits-Genome Mapping and Molecular Breeding in Plants 5. Vegetables. 2007:375.

Agatemor UM, Nwodo OFC, Anosike CA. Anti-inflammatory activity of Cucumis sativus L. British J. Pharm. Res. 2015; 8(2):1–8.

Zhu F, Du B, Xu B. Anti-inflammatory effects of phytochemicals from fruits, vegetables, and food legumes: A review. Critical reviews in food science and nutrition. 2018;58(8):1260-70.

Murugesh N, Vember S, Damondaran C. Studies on erythrocyte membrane IV: In-vitro haemolytic activity of oleander extract. Toxicology Letters. 1981;8:33-38.

Hossain MM, Ahamed SK., Dewan SMR, Hassan MM, Istiaq A, Islam MS, Moghal MMR. In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthes paniculata leaves. Biological Research. 2014;47(1):45.

Jameel MA, Gandasi RS, Praveen N, Biljo VJ, Fatima MA, Muneera QA. Flavonoids as Potential Anti-Inflammatory Molecules: A Review. Molecules. 2022;27(9):2901

Saleem TKM, Azeem AK, Dilip, C, Sankar C, Prasanth NV, Duraisami R. Anti-inflammatory activity of the leaf extacts of Gendarussa vulgaris Nees. Asian Pacific Journal of Tropical Biomedicine. 2011;1(2): 147–149.

Ojoghane E, Nwodo OFC. Comparison of extracts of Cyphostemma glaucophilla on total protein and membrane stabilization. Journal of Chemical and Pharmaceutical Research. 2010;2(4):31-37.

Mounnissamy VM, Kavimani S, Balu V, Drlin I, Quine S. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanol extract of Canjera rehedi. Iranian. Journal of Pharmacology and Therapeutics. 2008;6:235-237

Douglas BA, Daniels K, Isaac TH, Martins E. Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro. Int J Inflam. 2021;2021:1279359

David, S. Studies force new view on biology of flavonoids. Bio Medical. 2007; 541: 737-787.

Oyedapo OO. Biological activity of Plyllanthus amarus extracts on pragrow Dawley rats. Nigerian Journal of Biochemistry and Molecular Biology. 2001;16:83-86.

Agatemor UM, Nwodo OFC, Anosike CA. Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria. African Journal of Biotechnology. 2018;17(38):1215-1219.