Potential Effect of Soybean on Muscle Fatigue and Lactic Acid Levels in Wistar Rats
Aldona Putri Navolani
Department of Biology, Faculty of Teacher Training and Education Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Eka Nur Alfahrozi Putra
Department of Sport Science, Faculty of Health and Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Shefina Shintawati
Department of Pharmacy, Faculty of Health and Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Cicilia Novi Primiani *
Department of Pharmacy, Faculty of Health and Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Roy Try Putra
Department of Sport Science, Faculty of Health and Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Andri Wahyu Utomo
Department of Sport Science, Faculty of Health and Science, PGRI Madiun University, Madiun, East Java, Indonesia.
Gabriella C. Krisnamurti
Biotechnology Program, School of Bioresources and Tecnology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand.
Yadi Jayadilaga
Department of Sport Science, Faculty of Sport and Health Science, Makassar State University, Makassar, South Celebes, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
Muscle fatigue is a condition resulting from prolonged muscle contractions. Synthetic materials are often used to restore muscle fatigue, although they can have side effects. Soybean is a natural product that contains complex compounds, including thiamine, which has the potential to replace synthetic compounds. The current study was aimed to analyze the potential effect of Glycine max on the structure of muscle tissue/muscle fatigue and lactic acid levels in Wistar rats. The study used an experimental approach, the treatment of giving soybean powder and pure thiamine compounds to 24 male Wistar white rats aged 3 months totaling 24 rats, 150-200 g for 15 days. Data analysis techniques were carried out on the frequency of muscle fatigue through the struggling test, measurement of lactic acid levels in the blood, and structure of the biceps femoris muscle tissue in HE staining. The results showed struggling time, Fobs 16.398 > Ftable 3.554 and lactic acid levels Fobs 17.621 > Ftable 3.467 and changes in the muscle tissue structure of white rats. The conclusion that soybean seeds have the potential as a natural ingredient in overcoming muscle fatigue and can reduce lactic acid levels.
Keywords: Soybean, muscle fatigue, lactic acid, struggling, wistar rats