Effect of Dialysis on Biochemical Parameters in Chronic Renal Failure Patients: A Cross-sectional Study

Abdullah M. Albalawi

Laboratory, King Khalid University, Tabuk, Saudi Arabia.

Ayid A. Dera

Laboratory, King Khalid University, Tabuk, Saudi Arabia.

Prasanna Rajagopalan

Laboratory, King Khalid University, Tabuk, Saudi Arabia.

Sidra Maqsood *

Offices of Research, Innovation and Commercialization, Indus Hospital & Health Care, Karachi, Pakistan.

Filza S. Kashif

Laboratory, King Khalid University, Tabuk, Saudi Arabia.

Neelum Mansoor

Department of Hematology, Indus Hospital & Health Care, Karachi, Pakistan.

*Author to whom correspondence should be addressed.


Aims: Hemodialysis uses a semi-permeable membrane and electrochemical gradient to balance fluid and essential nutrient levels in the body. The kidneys perform this function in healthy individuals. The study aims to analyze the biochemical parameters of patients undergoing hemodialysis.

Study Design: Cross-sectional study.

Place and Duration of Study: The study enrolled 130 patients with chronic renal failure undergoing hemodialysis in King Khalid Hospital Tabuk, Saudi Arabia between January 2023 and February 2023.

Methodology: The study applied correlation analysis to determine the nature of the relationships between the biochemical parameters during hemodialysis. Descriptive statistics were also used to determine the mean concentrations for the parameters during hemodialysis.

Results: The concentrations of urea dropped from 45.12mmol/l from week one to 5.74mmol/l in week four. While the concentration of creatinine dropped from 761.53umol/l from week one to 160.60umol/l in week four. The study used correlation analysis to establish that there was a statistically significant strong positive correlation between urea and creatinine, calcium, iron, total iron binding capacity (TIBC), and albumin had their concentrations vary during dialysis weeks, with their concentrations increasing during some weeks and decreasing during others. It was also determined that there was a significant negative correlation between urea and TIBC.

Conclusion: The study established that there was a significant reduction in the concentration of urea and creatinine during the fourth week of dialysis compared to the first week of dialysis. Therefore, dialysis helps to remove toxic waste products from the bloodstream.

Keywords: Renal failure, hemodialysis, blood serum, creatinine, urea

How to Cite

Albalawi, Abdullah M., Ayid A. Dera, Prasanna Rajagopalan, Sidra Maqsood, Filza S. Kashif, and Neelum Mansoor. 2023. “Effect of Dialysis on Biochemical Parameters in Chronic Renal Failure Patients: A Cross-Sectional Study”. International Journal of Biochemistry Research & Review 32 (2):32-37. https://doi.org/10.9734/ijbcrr/2023/v32i2800.


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Venktapathy R, Govindarajan V, Oza N, Parameswaran S, Dhanasekaran BP, Prashad KV. Salivary creatinine estimation as an alternative to serum creatinine in chronic kidney disease patnties. IJN; 2014. Article ID 742724, 6.

Abdulla HI, Al-Kotany MY, Mahdi KA. Assessment of oral manifestations of patients with renal failure undergoing hemodialysis by serum and salivary biomarkers. MDJ. 2012;9(1):118–29.

Narula AS. Chronic kidney disease. The looming threat. MJAFI. 2008;64(1): 2–3.

Anupama YJ, Uma G. Prevalence of chronic kidney disease among adults in a rural community in South India: Results from the kidney disease screening (KIDS) project. IJN. 2014;24(4): 214–21.

Motamayel F.Ahmadi, Davoodi P., Dalband M., et al. Saliva as a Mirror of the Body Health. DJH. 2010;1(2)

Nandan Ratheesh K, B Sivapartha Sundaram, G Sivakumar. Oral manifestation and analysis of salivary and blood urea levels of patients undergoing haemodialysis and kidney transplant. Indian J dent Res. 2005;16:77–82.

Goll Robert D., Mookerjee Basab K. Correlation of biochemical parameters in serum and saliva in chronic azotemic patients and patients on chronic hemodialysis. Journal of Dialysis. 1978; 2:399–414.

Abdellatif Abeer M., Hegazy Salwa A., Youssef Jilan M., et al. The oral health status and salivary parameters of Egyptian children on haemodialysis. Journal of Advanced Research. 2011;2: 313–318.

Kaushik A, Reddy SS, Umesh L, Devi BK, Santana N, Rakesh N. Oral and salivary changes among renal patients undergoing hemodialysis: A cross-sectional study. Indian J Nephrol. 2013 Mar;23(2):125-9. DOI: 10.4103/0971-4065.109421

Vaidya SR, Aeddula NR. Chronic Renal Failure. [Updated 2021 Oct 29]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-. Available:https://www.ncbi.nlm.nih.gov/books/NBK535404/.

CF Streckfus, LR Bigler. Saliva as a diagnostic fluid. Oral Diseases. 2002; 8:69–76.

Molla MD, Degef M, Bekele A, Geto Z, Challa F, Lejisa T, Getahun T, Sileshi M, Tolcha Y, Ashebir G, Seifu D. Assessment of serum electrolytes and kidney function test for screening of chronic kidney disease among Ethiopian Public Health Institute staff members, Addis Ababa, Ethiopia. BMC Nephrol. 2020 Nov 18; 21(1):494.

DOI: 10.1186/s12882-020-02166-0.PMID: 33208123; PMCID: PMC7672884.

Fishbane S, Kalantar-Zadeh K, Nissenson AR. Serum ferritin in chronic kidney disease, Semin Dial. 2004;17:336 41.

Nisha R, Srinivasa Kannan SR, Thanga Mariappan K, et al. Biochemical evaluation of creatinine and urea in patients with renal failure undergoing hemodialysis. J Clin Path Lab Med. 2017;1(2):1-5.

Meenakshi GG. Effect of Dialysis on Certain Biochemical Parameters in Chronic Renal Failure Patients. IJCMR. 2016;3(10): 2869-2871.

Draczevski L, Teixeira ML. Avaliação do perfil bioquímicoe parâmetros hematológicos em pacientes submetidos à hemodiálise. Rev Saud Pesq. 2011;4:15-22.

Merzah KS, Hasson SF. The biochemical changes in patients with chronic renal failure. Int J Pharma Med Bio Sci. 2015;4: 75-79.

Entedhar RS, Nawal AM. Biochemical changes in chronic renal failure pre and post hemodilysis. J Environ Sci Eng Technol. 2016; 5:190-195.

Suresh M, Mallikarjuna RN, Sharan BSM, et al. Hematological changes in chronic renal failure. IJSRP. 2012;2:2250-3153.

Shivananda Nayak B. Manipal manual of clinical biochemistry. Jay Pee Brothers’ Medical Publishers (p) Ltd, New Delhi. 2002;98-99.