Proximate, Phytochemical and Mineral Composition of Methanol Extract of Persea americana Seed
International Journal of Biochemistry Research & Review, Volume 32, Issue 2,
Page 1-9
DOI:
10.9734/ijbcrr/2023/v32i2797
Abstract
This study investigated the proximate composition and evaluated the phytochemicals present in the methanol extract of Persea americana seed. The seed samples were collected, dried, ground into powder, and extracted in methanol by cold maceration. Proximate analysis was carried out using the standard methods of association of official analytical chemists (AOAC) while quantitative phytochemical analysis was done using standard methods. Mineral composition assays were carried out using the standard methods of the American public health association (APHA). Proximate analysis revealed that the seeds of P. americana contain more carbohydrate (32.05±1.21%), fat (25.73±2.01%), and protein (21.00±0.88%) with fewer amounts of moisture (8.98±0.10%), and ash (3.28±0.16%). Quantitative phytochemical analysis revealed the presence of phenols (3.51±0.42 mg/100 g), saponins (0.05±0.38 mg/100g), alkaloid (0.70±0.37 mg/100g), beta-carotene (32.24±0.55 mg/100 g), lycopene (6.84±0.52 mg/100 g), flavonoids (34.14±3.13 mg/ 100 g), phytate (0.00±0.03 mg/100 g), cardiac glycosides (0.37±0.01%), total phytosterol (21.25±0.12 mg/100 g), stigmasterol (13.16±0.15mg/100g) and campesterol (6.16±0.12 mg/100 g). Mineral contents of the sample revealed that potassium, calcium, and magnesium have the highest concentrations while manganese and iron have the lowest. The chemical compositions of the investigated samples might be responsible for their medicinal values in phytomedicine. This study shows that Persea americana seeds are adequate in lowering cholesterol levels probably due to the presence of phytosterols.
- Persea americana seeds
- phytochemical composition
- proximate
- minerals analysis
How to Cite
References
João JL, Érika HB, Rossana AC, Raimunda SN. José JC, Luciana MB, Selene MM, Marcos GR. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts. Revista da Sociedade Brasileira de Medicina Tropical. 2009;42(2):110-113.
Ajayi IA, Adesanwo O. Comparative study of the mineral element and fatty acid composition of Dacryodes edulis pulp and seed. World Journal of Agricultural Sciences. 2009;5:279-283.
Onuegbu NC, Nwuka MU, Ojukwu M, Kabu NO. Nutritional properties of African pear seed and performance of defatted cake in poultry feed formulations. Animal Nutrition. 2016;1(29):1-3.
Ortiz MA, Dorantes AL, Gallndez MJ, Cárdenas SE. “Effect of a novel oil extraction method on avocado (Persea americana Mill) pulp microstructure.” Plant Foods for Human Nutrition. 2004;59(1): 11–14.
Ferrari RA, Colussi F, Ayub RA. Characterization of by-products of passion fruit industralization. Revista Brasileira de Fruticultura. 2004;26:101-102.
Anaka ON, Ozolua RI, Okpo SO. Effect of the aqueous seed extract of Persea americana Mill (Lauraceae) on the blood pressure of sprague dawley rats. African Journal Pharmacy and Pharmacology. 2009;3(10):485-490.
Imafidon KE, Amaechina FC. Effects of aqueous seed extract of Persea americana Mill. (Avocado) on blood pressure and lipid profile in hypertensive rats. Advance Biological Researches. 2010;4(2):116-121.
Nwaoguikpe RN, Braide W. The effect of aqueous seed extract of Persea americana (avocado pear) on serum lipid and cholesterol levels in rabbits. African Journal of Pharmacy and Pharmacology Research. 2011;1(2):023-029.
Hirota Y, Nagao T, Watanabe Y, Suenaga M, Nakai S, Kitano M, Sugihara A, Shimada Y. Purification of steryl esters from soybean oil deodorizer distillate. Journal of the American Oil Chemists' Society. 2003;80:341-346.
Arukwe U, Amadi BA, Duru MKC, Agomuo EN, Adindu EA, Odika PC, Lele KC, Egejuru L, Anudike J. Chemical composition of Persea americana leaf, fruit and seed. International Journal of Research and Reviews in Applied Sciences. 2012;11(2):2-10.
Deepti D, Rachel MS, Gregory RZ, Joshua DL. Avocado (Persea americana) seed as a source of bioactive phytochemicals. Current Pharmaceutical Design. 2013;19:6133-6140.
Kumar AK, Ramachandra SS, Narsu L. Pharmacognostic and phytochemical investigation of roots of Hibiscus micranthus Linn. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2010;1(4):324-337.
AOAC. Official method of proximate analysis of food (17th ed) Association of Official Analytical Chemists, Arlington, Virginia, U.S.A; 1999.
Merrill AL, Watt BK. Energy value of foods: Basis and derivation. Agriculture Handbook No. 74. Washington, DC, ARS United State Department of Agriculture; 1973.
Trease GE, Evans WC. Phytochemicals. Pharmacognosy. 13th ed. ELBS/Bailliere Tindal, London. 1989:345–346.
Harborne JB. Phytochemical methods. A guide to modern techniques of plants analysis. 2nd Edition, Chapman and Hall, Ltd., London, UK. 1973:149-188.
Young SM, Greaves JS. Influence of variety and treatment on phytic acid content of wheat. Journal of Food Research. 1940;5:103-105.
Osagie AU. Antinutritional factors in nutritional quality of plant foods. In “Adeniyi SA, Orjiekwe CL, Ehiagbonare JE. Determination of alkaloids and oxalates in some selected food samples in Nigeria. African Journal of Biotechnology. 1998;8(1):110-112.
Narayan S, Amit RS, Bishun DP, Kanti S. Investigation on phytochemical, antimicrobial activity and essential oil constituents of Nardostachys jatamansi DC. In different regions of Nepal. Journal of Coastal Life Medicine. 2016;4(1):56-60.
Pearson D. The chemical analysis of foods. Churchhill Livingstone, Edinburgh. 6th Edition. 1974: 457.
Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Batista P. Total phenols, ascorbic acid, á-carotene and lycopene in portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry. 2007;103:413-419.
Larissa BDCA, Sarah LS, Marcos AMG, Magda RAF, Evani LA, Karina PR, Luiz AL. Total phytosterol content in drug materials and extracts from roots of Acanthospermum hispidum by UV-VIS spectrophotometry. Brazilian Journal of Pharmacognosis. 2013;23:736-742.
American Public Health Association (APHA). Standard methods for the examination of water and waste water, 19th edition APHA, Washington, D.C. 1995;1644.
Chikezie PC, Agomuo EN, Amadi BA. Proximate analysis In: Biochemistry Practical/Research method. A Fundamental Approach. Megasoft Publishers, Owerri. 2008;2:8-21.
Olusanya JO. Proteins, In: Essentials of food and nutrition. Apex Books Limited, Lagos. 2008:13-21.
Ifesan BOT, Olorunsola BO, Ifesan BT. Nutritional composition and acceptability of candy from avocado seed (Persea americana). International Journal of Agriculture Innovations and Researches. 2015;3(6):2319-1473.
Krogdahl A, Bakk-Mckellep AM. Physiological mechanism in the impairment of nutrient digestion and transport capacity in salmon intestine. Soybean Association Hudson Iowa, USA. 2002:128.
Ishida H, Suzunoh S, Sugiyana N, Innami S, Todoro T, Mackawa A. Nutritional evaluation of chemical components of leaves, stalk and stem of sweet potatoes (Ipomea botatus). Food Chemistry. 2000;68:359-367.
Dillard CJ, German EM. Coconut: In support of good health in the 21st century. 1990:127.
Duke J. Handbook of biological active phytochemicals and their activities. CRC Press, Boca Raton (Florida). 1992:99-131.
Salah W, Miller NJ, Pangauga T, Bolwell GP, Rice E, Evans C. Polyphenolic flavonols as scavengers of aqueous phase radicals as chainbreaking antioxidant. Archives of Biochemistry and Biophysics. 1995;2:339-346.
Stray F. The national guide to medicinal herbs and plants. Tiger Books International, London. 1988:1246.
Ramos MR, Jerz G, Villanueva S, Lopez-Dellamary F, Waibe R, Winterhalter P. Two glucosylated abscisic acid derivates from avocado seeds (Persea americana Mill. Lauraceae cv. Hass). Phytochemistry. 2004;65:955-962.
Okwu DE. Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. Journal 0f Sustainable Agriculture and Environment. 2004;6:30-34.
Okwu DE, Okwu ME. Chemical composition of spondiasmombia Linn plant parts. Journal of Sustainable Agriculture and the Environment. 2004;6:140-147.
Liener IE. Implications of antinutritional components in soybean foods. Critical Reviews in Food Science and Nutrition. 1994;34(1):31-67.
Sodipo OA, Akiniyi J. Studies on certain characteristics of extracts from bark of Pansinystaliamacruceras (K. schum). Pierre Exbeille. Global Journal of Pure and Applied Science. 2000;6:83-87.
Antia BS, Akpan EJ, Okon PA, Umoren IU. Nutritive and anti-nutritive evaluation of sweet potatoes leaves. Pakistan Journal of Nutrition. 2006;5(2):166-170.
Hurrel RF, Jullert MB, Reddy SR, Lynch SD, Cook JD. Changes in anti-nutrients contents of edible vegetables under varied temperature and heating time. American Journal of Clinical Nutrition. 1992;56:573.
Justina YT, Olukemi AO, Ajayi OO, Adegoke GO. Nutritional and antinutritional compositions of processed avocado (Persea americana Mill) seeds. Journal of Plant Science and Research. 2016;6(2):6-12.
Mohammad A, Koji Y, Toshiki M, Yomi N, Katsumi S, Hiroaki S, Yoshifumi T. Superoxide anion radical scavenging activities of herbs and pastures in northern Japan determined using electron spin resonance spectrometry. International Journal of Biological Sciences. 2007 3:349-355.
Susan DA. "Vitamin A", kirk-othmer encyclopedia of chemical technology (45). New York: John Wiley. 1998:99–107.
Niizuma H, Nakamura Y, Ozaki T, Nakanishi H, Ohira M. Bcl-2 is a key regulator for the retinoic acid-induced apoptotic cell death in neuroblastoma. Oncogene. 2006;25:5046-5055.
Evans WC. Traditional plant medicines as a source of new drugs. In Trease and Evans Pharmacognosy, 15th edition. Publishers, Elsevier. 2005:125-134.
Passmore R, Eastwood MA. In davison passmore, R., human nutrition and dietetics, Churchill Livingstone, London. 1986:124-126.
Fite B. The Healing miracle of coconut oil. Piccadilly Books Ltd, Healthwise Publications Colorado Springs, Co. 2000; 1-4.
-
Abstract View: 63 times
PDF Download: 59 times