Proximate, Phytochemical and Mineral Composition of Methanol Extract of Persea americana Seed

Omorovbiye Omorevbarhia *

Department of Biochemistry, Faculty of Natural Sciences, Paul University, Awka, Anambra State, Nigeria.

Sunday Chidozie Umerie

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

Ejike Louis Aniefuna

Department of Mineral Resources and Energy, Ministry of Petroleum and Mineral Resources, Awka, Anambra State, Nigeria.

Oguazu Chinenye Enoch

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Author to whom correspondence should be addressed.


This study investigated the proximate composition and evaluated the phytochemicals present in the methanol extract of Persea americana seed. The seed samples were collected, dried, ground into powder, and extracted in methanol by cold maceration. Proximate analysis was carried out using the standard methods of association of official analytical chemists (AOAC) while quantitative phytochemical analysis was done using standard methods. Mineral composition assays were carried out using the standard methods of the American public health association (APHA).  Proximate analysis revealed that the seeds of P. americana contain more carbohydrate (32.05±1.21%), fat (25.73±2.01%), and protein (21.00±0.88%) with fewer amounts of moisture (8.98±0.10%), and ash (3.28±0.16%). Quantitative phytochemical analysis revealed the presence of phenols (3.51±0.42 mg/100 g), saponins (0.05±0.38 mg/100g), alkaloid (0.70±0.37 mg/100g), beta-carotene (32.24±0.55 mg/100 g), lycopene (6.84±0.52 mg/100 g), flavonoids (34.14±3.13 mg/ 100 g), phytate (0.00±0.03 mg/100 g), cardiac glycosides (0.37±0.01%), total phytosterol (21.25±0.12 mg/100 g), stigmasterol (13.16±0.15mg/100g) and campesterol (6.16±0.12 mg/100 g). Mineral contents of the sample revealed that potassium, calcium, and magnesium have the highest concentrations while manganese and iron have the lowest. The chemical compositions of the investigated samples might be responsible for their medicinal values in phytomedicine. This study shows that Persea americana seeds are adequate in lowering cholesterol levels probably due to the presence of phytosterols.

Keywords: Persea americana seeds, phytochemical composition, proximate, minerals analysis

How to Cite

Omorevbarhia, Omorovbiye, Sunday Chidozie Umerie, Ejike Louis Aniefuna, and Oguazu Chinenye Enoch. 2023. “Proximate, Phytochemical and Mineral Composition of Methanol Extract of Persea Americana Seed”. International Journal of Biochemistry Research & Review 32 (2):1-9.


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