Therapeutic Effects of Pomegranate (Punica granatum L.) Juice on Liver of Diabetic Mice
- Maha K. Aljuaid
- Hayat Ashi
- Rahaf M. Fadul
- Raghad Al Mutawea
- Nouf Alqethami
- Israa Fawtah
- Asmaa H. Al-Robiee
-
Enas A. Hamed
International Journal of Biochemistry Research & Review, Volume 31, Issue 10,
Page 49-59
DOI:
10.9734/ijbcrr/2022/v31i10786
Abstract
Diabetes Mellitus (DM) is a serious metabolic disorder. Pomegranate has various biological effects. The present study evaluated pomegranate juice (PJ) therapeutic effects on the liver of Streptozotocin (STZ) induced DM mice model. Fifty male mice were distributed into 5 equal groups (10 each). Groups were negative control, PJ group received orally PJ (180 mg/kg), diabetic group received STZ (60 mg/kg), the diabetic received pomegranate juice (STZ+PJ) group, the diabetic received metformin (250 mg/kg) (STZ+MET) group. The experiment duration was 6 weeks. Initial and final body weights and liver weights were measured. At the experimental end, blood glucose levels and liver enzymes serum levels as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured. A liver histological examination was made under a light microscope. STZ led to a significant decreased in final body weight, and an increase in blood glucose and serum levels of AST, ALT, and ALP versus control. PJ administration to diabetic mice significantly decreased blood glucose and serum levels of liver enzymes versus the STZ group but AST and ALP levels were still elevated versus control. Histopathological liver examination of PJ-and MET-treated groups showed amelioration of histological changes of DM. Metformin repairs damaged hepatic cells but was not as effective as PJ. In conclusion, pomegranate juice improves liver health in diabetic mice.
- Diabetes mellitus
- liver
- metformin
- mice
- pomegranate juice
How to Cite
References
Ferrell JM, Chiang JY. Circadian rhythms in liver metabolism and disease. Acta pharmaceutica Sinica B. 2015;5(2):113-22.
Kwo PY, Cohen SM, Lim JK. ACG clinical guideline: evaluation of abnormal liver chemistries. Official journal of the American College of Gastroenterology| ACG. 2017;112(1):18-35.
Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature reviews Endocrinology. 2018;14(2):88-98.
Abdulaziz Al Dawish M, Alwin Robert A, Braham R, Abdallah Al Hayek A, Al Saeed A, Ahmed Ahmed R, et al. Diabetes mellitus in Saudi Arabia: a review of the recent literature. Current diabetes reviews. 2016;12(4):359-68.
Rodrigues B, Poucheret P, Battell ML, McNeill JH. Streptozotocin-induced diabetes: induction, mechanism (s), and dose dependency. Experimental models of diabetes: Routledge; 2018. p. 3-17.
Matthaeus B, Ozcan MM. Pomegranate plant (Punica granatum L.) composition, antioxidant activity, therapeutic effect, antimicrobial activity-A review. Zeitschrift Fur Arznei-& Gewurzpflanzen. 2016;21(4):160-7.
Amri Z, Lazreg-Aref H, Mekni M, El-Gharbi S, Dabbaghi O, Mechri B, et al. Oil characterization and lipids class composition of pomegranate seeds. BioMed research international. 2017;2017.
Pirzadeh M, Caporaso N, Rauf A, Shariati MA, Yessimbekov Z, Khan MU, et al. Pomegranate as a source of bioactive constituents: A review on their characterization, properties and applications. Critical reviews in food science and nutrition. 2021;61(6):982-99.
Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of ethnopharmacology. 2007;109(2):177-206.
Aviram M, Dornfeld L, Kaplan M, Coleman R, Gaitini D, Nitecki S, et al. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs under experimental and clinical research. 2002;28(2-3):49-62.
Lee J, Watson RR. Pomegranate: a role in health promotion and AIDS? Nutrients and Foods in AIDS: CRC Press. 2017;213-6.
Harzallah A, Hammami M, Kępczyńska MA, Hislop DC, Arch JR, Cawthorne MA, et al. Comparison of potential preventive effects of pomegranate flower, peel and seed oil on insulin resistance and inflammation in high-fat and high-sucrose diet-induced obesity mice model. Archives of Physiology and Biochemistry. 2016; 122(2):75-87.
Faddladdeen K, Ojaimi A. Protective Effect of Pomegranate (Punica granatum) Extract against Diabetic Changes in Adult Male Rat Liver: Histological Study. Journal of microscopy and ultrastructure. 2019;7(4):165-70.
Li Y, Qi Y, Huang TH, Yamahara J, Roufogalis BD. Pomegranate flower: a unique traditional antidiabetic medicine with dual PPAR‐α/‐γ activator properties. Diabetes, Obesity and Metabolism. 2008;10(1):10-7.
Huang TH, Yang Q, Harada M, Li GQ, Yamahara J, Roufogalis BD, et al. Pomegranate flower extract diminishes cardiac fibrosis in Zucker diabetic fatty rats: modulation of cardiac endothelin-1 and nuclear factor-kappaB pathways. Journal of cardiovascular pharmacology. 2005;46(6):856-62.
Yuan T, Ding Y, Wan C, Li L, Xu J, Liu K, et al. Antidiabetic ellagitannins from pomegranate flowers: Inhibition of α-glucosidase and lipogenic gene expression. Organic letters. 2012;14(20): 5358-61.
Sangi SMA, Bawadekji A, Alotaibi NM, Aljalaud NA. Preventive and Curative Effects of Metformin, Nigella sativa, Punica granatum and Zingiber officinale on Male Reproductive Dysfunction in Diabetic Rats. International Journal of Pharmaceutical Research & Allied Sciences. 2019;8(2): 48-57.
Zhou B, Zhang Y, Li S, Wu L, Fejes-Toth G, Naray-Fejes-Toth A, et al. Serum-and glucocorticoid-induced kinase drives hepatic insulin resistance by directly inhibiting AMP-activated protein kinase. Cell reports. 2021;37(1):109785.
Ilame SA, V. Singh S. Application of membrane separation in fruit and vegetable juice processing: a review. Critical Reviews in Food Science and Nutrition. 2015;55(7):964-87.
Ragbetli C, Ceylan E. Effect of streptozotocin on biochemical parameters in rats. Asian journal of chemistry. 2010;22(3):2375.
Taheri Rouhi SZ, Sarker M, Rahman M, Rahmat A, Alkahtani SA, Othman F. The effect of pomegranate fresh juice versus pomegranate seed powder on metabolic indices, lipid profile, inflammatory biomarkers, and the histopathology of pancreatic islets of Langerhans in streptozotocin-nicotinamide induced type 2 diabetic Sprague–Dawley rats. BMC Complementary and Alternative Medicine. 2017;17(1):1-13.
Ye J, Luo D, Xu X, Sun M, Su X, Tian Z, et al. Metformin improves fertility in obese males by alleviating oxidative stress-induced blood-testis barrier damage. Oxidative medicine and cellular longevity. 2019;2019:9151067.
Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society. 2016;24(5):547-53.
Masarone M, Rosato V, Dallio M, Gravina AG, Aglitti A, Loguercio C, et al. Role of Oxidative Stress in Pathophysiology of Nonalcoholic Fatty Liver Disease. Oxid Med Cell Longev. 2018;2018:9547613.
Hassan G, Abdel Moneium T. Structural changes in the testes of streptozotocin-induced diabetic rats. Suez Canal Univ Med J. 2001;4(1):17-25.
Mallick C, Mandal S, Barik B, Bhattacharya A, Ghosh D. Protection of testicular dysfunctions by MTEC, a formulated herbal drug, in streptozotocin induced diabetic rat. Biological and Pharmaceutical Bulletin. 2007;30(1):84-90.
Pedersen C, Porsgaard T, Thomsen M, Rosenkilde MM, Roed NK. Sustained effect of glucagon on body weight and blood glucose: Assessed by continuous glucose monitoring in diabetic rats. PloS one. 2018;13(3):e0194468.
Al-Muammar MN, Khan F. Obesity: the preventive role of the pomegranate (Punica granatum). Nutrition. 2012;28(6):595-604.
Estrada-Luna D, Martínez-Hinojosa E, Cancino-Diaz J, Belefant-Miller H, López-Rodríguez G, Betanzos-Cabrera G. Daily supplementation with fresh pomegranate juice increases paraoxonase 1 expression and activity in mice fed a high-fat diet. European journal of nutrition. 2018;57(1):383-9.
Amin KA, Kamel HH, Abd Eltawab MA. The relation of high fat diet, metabolic disturbances and brain oxidative dysfunction: modulation by hydroxy citric acid. Lipids in health and disease. 2011;10(1):1-11.
Nwozo SO, Orojobi BF, Adaramoye OA. Hypolipidemic and antioxidant potentials of Xylopia aethiopica seed extract in hypercholesterolemic rats. Journal of Medicinal Food. 2011;14(1-2):114-9.
Gabr M. Effects of pomegranate (Punica granatum L.) fresh juice and peel extract on diabetic male albino rats. Al-Azhar Medical Journal. 2017;46(4):965-80.
Papaccio G, Pisanti FA, Latronico MV, Ammendola E, Galdieri M. Multiple low‐dose and single high‐dose treatments with streptozotocin do not generate nitric oxide. Journal of cellular biochemistry. 2000;77(1):82-91.
Sohrab G, Roshan H, Ebrahimof S, Nikpayam O, Sotoudeh G, Siasi F. Effects of pomegranate juice consumption on blood pressure and lipid profile in patients with type 2 diabetes: A single-blind randomized clinical trial. Clinical nutrition ESPEN. 2019;29:30-5.
Alves M, Martins A, Vaz C, Correia S, Moreira P, Oliveira P, et al. Metformin and male reproduction: effects on S ertoli cell metabolism. British Journal of Pharmacology. 2014;171(4):1033-42.
Al-Sallami A, Al-Bideri AW, Alsaaedi SH. Hepatoprotective effect of pomegranate peel (Punica granatum L) against thioacetamide-induced cirrhosis. The Egyptian Journal of Experimental Biology (Zoology). 2018;14:41-7.
Toklu HZ, Sehirli O, Sener G, Dumlu MU, Ercan F, Gedik N, et al. Pomegranate peel extract prevents liver fibrosis in biliary-obstructed rats. Journal of Pharmacy and Pharmacology. 2007;59(9):1287-95.
Contreras-Zentella ML, Hernández-Muñoz R. Is liver enzyme release really associated with cell necrosis induced by oxidant stress? Oxidative medicine and cellular longevity. 2016;2016.
Zhai X, Zhu C, Zhang Y, Sun J, Alim A, Yang X. Chemical characteristics, antioxidant capacities and hepatoprotection of polysaccharides from pomegranate peel. Carbohydrate polymers. 2018;202:461-9.
Bassiri-Jahromi S. Punica granatum (Pomegranate) activity in health promotion and cancer prevention. Oncology reviews. 2018;12(1):345-52.
Aboonabi A, Rahmat A, Othman F. Effect of pomegranate on histopathology of liver and kidney on generated oxidative stress diabetic induced rats. J Cytol Histol. 2014;6(1):2-5.
Al-Attar AM, Alsalmi FA. Influence of olive leaves extract on hepatorenal injury in streptozotocin diabetic rats. Saudi journal of biological sciences. 2019;26(7):1865-74.
KAJ F. Ameliorating effect of pomegranate peel extract supplement against type 1 diabetes-induced hepatic changes in the rat: biochemical, morphological and ultrastructural microscopic studies. Folia Morphologica. 2021;80(1):149-57.
Rodríguez V, Plavnik L, Tolosa de Talamoni N. Naringin attenuates liver damage in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie. 2018; 105:95-102.
Maritim AC, Sanders RA, Watkins JB, 3rd. Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology. 2003;17(1):24-38.
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