LC-MS, GC-MS and Hematological Profile of Mucuna pruriens Extracts in Alloxan Induced Diabetic Albino Rat
E. C. Chukwu
Department of Biochemistry, Federal University of Lafia, Nasarawa State, Nigeria.
K. U. Osuocha *
Biochemistry Unit, Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa State, Nigeria.
B. Musa
Centre for Dry Land Agriculture, Bayero University Kano, Nigeria.
J. C. Njoku
Department of Pharmacology, Niger Delta University , Bayelsa State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Mucuna pruriens also known as velvet beans is an annual climbing shrub that has been claimed traditionally to possess anti anemic potentials. The present study evaluated bioactive constituents and hematological profile of Mucuna pruriens seed and leaf extracts on alloxan induced diabetic male albino rat using standard analytical method. Result of LC-MS investigation on the seed shows the presence of Hydroxystreptozolin ( Oxazolidene) and Dihydrocapsaicin (Meltoxyphe.nol) while the leaf reveals the presence of four(4) bioactive compound: Furocoumurine acid, Epioxylubianin, Acetyllaustoinid and Quassin. The GC-MS studies on Mucuna pruriens seed indicates the presence of twelve bioactive compound. 2-methyl-z,z-3,13-octadecadienol cis-13-octadecadienoic acid, methylester hexadecanoic acid, methylester pentadecanoic acid, 9,12,octadecadienoic acid (z, z)-methylester, 11-octadenoic acid, methylester q-octadecenoic acid, Octadecanamide- N-(2-hydroxyethyl) dodecanamide,N-(2-hydroxyethyl), urea,triethyl-Ethanamine, N, N-dimethyl (phenylmethoxy), 13-Hexyloxacyclotridec carbonitrile,3,5bis[C2dimethylamino]ethylthio, 12-octadecadienoic acid(z, z)-cis-13-octadecenoic acid, 9,12-octadecadienoicacid(z,z)-2-hydroxy-1(Hydroxymethyl) ethylester, cyclopentadecanane,2-hydroxy-9,12-octadeadienol,LE, etc while GC-MS analysis of the leaf reveals the presence of twenty bioactive compounds such as Nonyl alcohol, 3-Undecene, 1-Dodecene etc. Findings from this study indicates significant improvement on the hematological indices in treated groups relative to untreated. The restorative hematological efficacy of Mucuna pruriens extract is indicative of its traditional relevance in medicinal and therapeutic applications and hence may justify its local claims as having anti-anemic functionality.
Keywords: Mucuna pruriens, hematological, profile, alloxan