Cytotoxicity and Genotoxicity of Sunset Yellow and Potassium Sorbate in Jurkat Cell Line
D. Halilčević *
University of Tuzla, Faculty of Pharmacy, Department of Biochemistry, Bosnia and Herzegovina.
E. Dautović
University of Tuzla, Faculty of Pharmacy, Department of Biochemistry, Bosnia and Herzegovina.
M. Lelić
University of Tuzla, Faculty of Medicine, Department of Histology, Bosnia and Herzegovina.
M. Šljivić Husejnović
University of Tuzla, Faculty of Pharmacy, Department of Pharmaceutical Analytics, Bosnia and Herzegovina.
A. Smajlović
University of Tuzla, Faculty of Pharmacy, Department of Biochemistry, Bosnia and Herzegovina.
N. Srabović
University of Tuzla, Faculty of Pharmacy, Department of Biochemistry, Bosnia and Herzegovina.
A. Softić
University of Tuzla, Faculty of Pharmacy, Department of Biochemistry, Bosnia and Herzegovina.
*Author to whom correspondence should be addressed.
Abstract
Aims: Industrial processed food, pharmaceutical and cosmetical products contain numerous substances the safety of which has been widely concerned. Potassium sorbate (PS) is a common preservative used in a wide-range of products. Sunset yellow (SY) is one of the most commonly used dye in food and pharmaceutical industry. Several studies suggested cytotoxic and genotoxic potential of SY and PS in different cell lines. These effects can lead to organ damage and cancer development. The aim of this study is to investigate cytotoxic and genotoxic potential of two widely used food additives, SY and PS, in Jurkat cell line.
Methodology: PrestoBlue assay was used to assess the cytotoxic potential of SY and PS. For observation of DNA damage, cytostatic and cytotoxic effects, the cytokinesis-block micronucleus cytome (CBMN) assay was performed.
Results: Decrease of cell viability in Jurkat cell line was observed after 24-hour exposure to both SY and PS. CBMN assay has revealed significant increase of necrotic cells (P<0.05). Genotoxic biomarkers were in physiological range after 24-hour exposure to both analyzed additives.
Conclusion: Our findings suggest that SY, as well as PS have cytotoxic potential in Jurkat cell line, as a result of increased number of necrotic cells. Higher cytotoxic effect was caused by SY compared to PS. However, genotoxic potential was not recorded for any of the food additives analyzed.
Keywords: Cytotoxic effect, genotoxic effect, dye sunset yellow, preservative potassium sorbate, food additives