Aminogram in Egyptian Patients Experiencing Myocardial Infarction
Tahia H. Saleem *
Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Ayat A. Sayed
Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt and Biochemistry Department, Faculty of Pharmacy, Sphinx University, Assiut, Egypt.
Mohamed Abdel Ghany
Cardiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Ahmed Farouk
Cardiothoracic Surgery Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Ahmed H. Hussein
Medical Biochemistry Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Abdel-Raheim M.A. Meki
Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt and Biochemistry Department, Faculty of Pharmacy, Sphinx University, Assiut, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: Myocardial infarction is one of the leading causes of death worldwide. Altered amino acids metabolism is linked to cardiovascular disorders. A cross-sectional case control study was set to profile amino acids explore their potential as diagnostic and prognostic tool.
Methods: Total of 60 subjects was recruited, comprising 40 myocardial infarction patients and 20 controls. High pressure liquid chromatography was applied to quantify the plasma amino acids.
Results: Fourteen amino acids exhibited discriminative values between cases and controls. Leucine, isoleucine, methionine, glycine, threonine, serine, ornithine, arginine, histidine, and tyrosine exhibited low levels compared to healthy controls (p ≤0.001). Meanwhile, cases displayed higher abundance of cysteine, taurine (p ≤ 0.05), total aromatic amino acids (p ≤ 0.01), and asparagine (p ≤ 0.001). Only leucine, isoleucine, and asparagine were correlated to GRACE risk score. Methionine and glycine scored highly (AUC > 0.95), sensitivity (>97%), and specificity (>95%) followed by histidine (AUC > 0.92), sensitivity (>82%), and specificity (>85%).
Conclusion: Our study designated a panel of 14 plasma circulating amino acids. Methionine, glycine and histidine were found to be potential markers to differentiate cases from healthy Individuals. Plasma amino acids profiling could help in the diagnosis but is less powerful as a prognostic tool of Myocardial infarction.
Keywords: Amino acids, myocardial infarction, cardiovascular disorders, liquid chromatography