Bioassay Guided Screening of Cryptolepis Extracts for Onward Synthesis of Nano Ferrites
Hafza Murtaza *
The University of Lahore, Sargodha Campus, Pakistan.
Aasia Sikander
The University of Lahore, Sargodha Campus, Pakistan.
Umema Murtaza
The Women University Multan, Pakistan.
Ashir Masroor
University of Agriculture Faisalabad Sub-Campus Burewala, Pakistan.
Filza Ghafoor
Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
A study was designed to investigate the presence of nanoferrites in Cryptolepis buchanani. In this study, various leaves and stem organic extracts were prepared separately using the solvents viz., n-hexane, ethyl acetate, ethanol and water. Different phytochemical tests for alkaloids, carbohydrates, oils, amino acids and others were performed to determine their presence in the extracts. These plant extracts were used for screening of sample via UV and HPLC techniques to compare the wavelength and absorbance, and retention time on chromatogram by extracts, respectively. The results showed the presence of alkaloids, carbohydrates and proteins in all the samples. However, other compounds were present randomly. Spectroscopy showed highest peaks with ethyl acetate extracts and the lowest with water. The surface properties and size of nickel-zinc nano ferrites were evaluated by Energy-dispersive X-ray spectroscopy (EDX) and Scanning Electron Microscopy (SEM). The former, showed the presence of nickel (Ni) and zinc (Zn) weighing 4.27⁒ of N ,6.89⁒ of C and 35.5⁒ of O in the sample which confirmed the presence of nano ferrites in leaves and stem of C. buchanani.
Keywords: Cryptolepis buchanani, phytochemistry, plant analysis, phytochemicals, nano ferrites, nickel-zinc compounds