Possible Antioxidant and Haematinic Properties of the Stem Bark of Theobroma cacao L. in Wistar Rats

B. O. Oluwatayo *

Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria and Department of Physiology, Madonna University Elele Rivers State, Nigeria.

T. A. Kolawole

Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Rivers, Nigeria.

C. C. Wali

Department of Anatomy, Baze University, Abuja, Federal Capital Teritory, Abuja, Nigeria.

O. A. Olayanju

Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, Nigeria.

A. E. J. Okwori

Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Background: This study investigated the potential antioxidant effects of aqueous extract of the stem bark of Theobroma cacao L. in Wistar rats.

Methods: Twenty Wistar rats weighing between 126 g – 224 g were grouped randomly into 4groups of 5 rats each. Group 1 served as control and received water while groups 2, 3 and 4 rats were given 1000mg/kg, 3000mg/kg and 5000mg/kg b.wt of the extract respectively for 28days. On the 29th day, the rats were anaesthetized and blood samples were collected for analysis of some haematological parameters, enzymatic and non- enzymatic antioxidant activities.

Results: The results obtained showed that there was significant increase (p<0.001) in SOD, Catalase activities and MDA levels in a dose dependent manner. The results also showed significant increase (p<0.001) in RBC Group 2, 3 and 4 rats when compared to the Group1. Significant increase was also observed in Hemoglobin (Hb) and Hematocrit (Hct) level in group 2 and 3 rats (p<0.001). Mean corpuscular volume was significantly increased in group 2 rats (p<0.001).

Conclusion: The findings from this study showed the antioxidant and hematinic potentials of the stem bark of Theobroma cacao L.The aqueous extract of the stem bark of Theobroma cacao L. has a potential antioxidative and hematinic effects in Wistar rats. This is largely due to its rich phytochemical and nutritive contents. Further research work will be needed to see the possible application of these properties in humans.

Keywords: Theobroma cacao L., antioxidant properties, haematological parameters, wistar rats


How to Cite

Oluwatayo, B. O., T. A. Kolawole, C. C. Wali, O. A. Olayanju, and A. E. J. Okwori. 2021. “Possible Antioxidant and Haematinic Properties of the Stem Bark of Theobroma Cacao L. In Wistar Rats”. International Journal of Biochemistry Research & Review 30 (3):18-26. https://doi.org/10.9734/ijbcrr/2021/v30i330256.

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