Phenolic Content, Antioxidant and 15-Lypoxygenase Inhibitory Effects of Daniellia oliveri (rolfe) Hutch. & Dalziel (Fabaceae)
Mariam Traore *
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
Adjaratou C. Coulibaly
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
Aboubacar Yaro
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.
Esther W. L. M. Kabre
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
Noufou Ouedraogo
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.
Martin Kiendrebeogo
Institut de Recherche en Sciences de la Santé (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
Richard W. Sawadogo
Laboratoire de Biochimie et Chimie Appliquée (LABIOCA), Ecole Doctorale Sciences et Technologie, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of this study is to evaluate the content of phenolic compounds, antioxidant and 15-lipoxygenase inhibition effects of methanolic extract and its fractions of Daniellia. oliveri stem bark.
Place and Duration of Study: The work was carried out in the Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Institute for Research in Health Sciences (IRSS) and LABIOCA from Université Joseph KI-ZERBO in Ouagadougou between February 2018 and June 2019
Methodology: The phytochemical screening and phenolic content were determined by the methods described in the literature. Antioxidant activity was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis 3-ethylbenzoline-6-sulphonate), FRAP (ferric reducing antioxidant power), and lipid peroxidation (LPO). The anti-inflammatory activities were measured on the ability of the extract to inhibit the activity of enzymes such as 15-lipoxygenase
Results: Phytochemical screening revealed the presence of flavonoids, tannins, saponins, anthocyanosides, triterpenes and sterols. The extract and its fractions showed a good content of phenolic compounds with values ranging from 773.97 ± 22.91 to 505.51 mg EAG / g for total polyphenols; from 24.38 ± 0.34 to 12.04 ± 0.08 mg EQ / g in total flavonoids, 311.65 ± 6.70 to 221 ± 0.52 mg EC / g in condensed tannins; 681.81 ± 3.97 to 421.29 ± 8.73 mg EUA / g in total triterpenes and from 280.29 ± 21.72 to 222.04 ± 20.99 mg EC / g for total sterols. The methanol fraction showed the best antioxidant activity as well as the best inhibitory effect on 15-lipoxygenase activity.
Conclusion: These results showed that Daniellia. oliveri possessed an antioxidant power, an inhibitory effect of 15 lipoxygenase. Daniellia oliveri could be used in the management of inflammatory pathologies and oxidative stress.
Keywords: Daniellia oliveri, antioxidant, 15-lipoxygenase, anti-inflammatory, phenolic compounds