GC-MS Analysis of Spondias mombin (Linn) Methanol Leaf Extract
S. C. Okereke
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
O. V. Ikpeazu
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
C. J. Nwaogwugwu *
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
A. S. Ezekwe
Department Medical Biochemistry, College of Medical Sciences, River State University, Port Harcourt, Nigeria.
C. I. Nosiri
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
P. O. Ukogu
Department of Chemistry, Faculty of Physical Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
Duru Michael Osita
Department of Biochemistry, Imo State University, Owerri, Nigeria.
Akara Emmanuel
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, P.M.B. 2000, Uturu, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The bioactive components of Spondias mombin leaf have been evaluated using gas chromatography and mass spectroscopy (GC/MS). The plant were collected washed, shade dried and milled and the methanol extract of the leaf plant were prepared by soxhlet extract method. The methanol extract were analyzed for the identification of phytochemical compounds presenting the Spondias mombin plant leaf using GC/MS methods. Result of the GC-MS analysis of Spondias mombin leaf extract leads to the identification of 8 compounds. The identification of phytochemicals was based on the Molecular Weight, molecular formula, RT (Retention Time) and peak area % (concentration). These peaks indicated the presence of eight bioactive compounds in the extract. The names, molecular formular, percentage composition and molecular masses of these compounds are shown in Table 1. The 8 bioactive compounds are as follows; 9- oxononanoic acid,3-isopropoxy, 1,1,15,5,5 hexamethyl-3- trimethylsioxytrosiloxane (C12H34O4Si4) ,1,4-methanocycloocta(d)pyridazine, 1α4α4aα10α)(C13H2ON2),Hexasiloxane 1,1,3,3,5,5,7,7,9,9,11,11-dodeca methyl,1,4 methanocycloocta (d) pyridazine 1,4,4a,5,6,9,10,10a- octalydro 11,11-dimethyl-(1α,4α4aα10α), Hexasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11 dodecamethyl, 3- isopropoxy-1,1,1,5,5,5 hexamethyl-3-(trimethylsiloxy) trisiloxane C12H34O4Si4 and 1,1,1,3,5,5,5- Heptamethyltrisiloxane(C7H22O2Si3). It is concluded that these bioactive component are known to exhibit medicinal value as well as pharmacological activities.
Keywords: Phytochemicals, gas-chromatography, Spondias mombin, bioactive, phytochemical.