Cardiac Tolerance Study of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) Extract in Rabbit
Irie Otis Tra-Bi *
Biochemical Pharmacodynamy Laboratory, Biosciences Department, University Felix Houphouet Boigny PO Box 582, Abidjan 22, Ivory Coast
Bernard Nazaire Djyh
Biochemical Pharmacodynamy Laboratory, Biosciences Department, University Felix Houphouet Boigny PO Box 582, Abidjan 22, Ivory Coast
Amadou Founzegue Coulibaly
Biochemical Pharmacodynamy Laboratory, Biosciences Department, University Felix Houphouet Boigny PO Box 582, Abidjan 22, Ivory Coast and Pasteur Institute of Ivory Coast, PO Box 2393, Abidjan 04, Ivory Coast
Allico Joseph Djaman
Biochemical Pharmacodynamy Laboratory, Biosciences Department, University Felix Houphouet Boigny PO Box 582, Abidjan 22, Ivory Coast and Pasteur Institute of Ivory Coast, PO Box 2393, Abidjan 04, Ivory Coast
Jean David N’guessan
Biochemical Pharmacodynamy Laboratory, Biosciences Department, University Felix Houphouet Boigny PO Box 582, Abidjan 22, Ivory Coast
*Author to whom correspondence should be addressed.
Abstract
Purpose: This study was conducted to assess the effect of the ethyl acetate fraction (AcEF1) obtained from the 96% extract of Morinda morindoides extract on cardiac tissue integrity and function in rabbits.
Methodology: The rabbits were divided in five groups of 6 rabbits each. Groups 2,3,4 and 5 received intraperitoneally, twice a week during four weeks, the ethyl acetate fraction at doses going from 25 to 100 μg/kg body weight while Group 1, received 1 mL of Mac Ewen fluid. Blood sampling was carried out to evaluate aspartate aminotransferase (AST), alanine aminotransferase (ALT), Creatine phosphokinase (CPK), Lactate dehydrogenase (LDH) activities and level of calcium, sodium, potassium, magnesium and chloride in serum. The eighth week of the experiments, histopathological studies were also conducted on rabbits heart.
Results: Analysis of the serum markers showed slight increases in AST, ALT, CPK activities as well as sodium, calcium and potassium concentrations (p<0.05). LDH activity, magnesium and chloride concentrations were unchanged compared to their initial values.
Histopathological studies had not revealed damages in the structure of the heart.
Conclusion: The ethyl acetate fraction did not exert any noxious effect on cardiac tissues and should be no toxic for cardiac tissues.
Keywords: Biochemical markers, Cardiac tolerance, Histopathology, Morinda morindoides