Assessment of the Wound Healing Property of the Ethanolic Seed Extract of Eleusine coracana Linn using the Chorioallantoic Membrane Model
International Journal of Biochemistry Research & Review,
Access to readily available material for wound healing is very important. The wound healing property of Eleusine coracana a food source in Nigeria was therefore investigated. The ethanolic crude extract of the seed flour was prepared using maceration and qualitative phytochemical screening was carried out on the crude extract using standard methods. The antimicrobial activity of the E. coracana extract was determined using the disc diffusion method against E. coli, S. aureus, P. aeruginosa, and C. tetani. The wound healing property assay was carried out using the Chorioallantoic Membrane Model (CAM) at 50mg, 100mg, 150mg, and 200mg/ml concentration with the basic Fibroblast Growth Factor (bFGF) as the positive control. The crude extract was further fractionated into Hexane, Methanolic, and Aqueous fractions and then subjected to amino acid content analysis using the Applied Biosystems Phenylthiohydantoin (PTH) Amino Acid Analyzer. The phytochemical screening reveals the presence of alkaloids, steroids, terpenes, flavonoids, carbohydrates, glycoside, and protein. E. coli and S. aureus were susceptible to the E. coracana extract at a Minimum Inhibitory Concentration (MIC) of 50µg/ml while the P. aeruginosa was only susceptible at 200µg/ml and C. tetani was not susceptible at all the concentrations used. An increase in percentage angiogenesis was observed at all doses of the extract used indicating good wound healing properties. The amino acid profile reveals the presence of glycine, a proline that is important in wound healing. The E. coracana seeds, therefore, have wound healing properties based on the CAM model.
- wound healing
- E. coracana
- amino acid
How to Cite
Menke NB, Ward KR, Witten TM, Bonchev DG, Diegelmann RF. Impaired Wound Healing. Clin Dermatol. 2007;25:19–25.
Baranoski S, Ayello EA. Wound Dressings: An Evolving Art and Science. Adv Skin Wound Care. 2012;25:87–92.
Benbow M. Debridement: Wound Bed Preparation. J Community Nurs. 2011;25:18–23.
Upadhyaya HD, Gowda CLL, Reddy VG. Morphological Diversity in Finger Millet Germplasm introduced from Southern and Eastern Africa. J. SAT Agric. Res. 2007;3(1):1–3.
Kareem AM, Bello SO, Etuk E, Arisegi SA, Umar MT. Evaluation of the hypoglycemic and hypolipidemic effects of aqueous Eleusine coracana seed extract. International archives of medicine and medical sciences. 2019;1(3):35-41.
Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies. 2020;8(2):603-608.
Harbone JB. Phytochemical Methods, 3rd Ed. Chapman and Hall: London. 1988;135-203.
Singh M, Naithani M. Phytochemical Estimation and Antioxidant Activity of Seed Extract of Millets Traditionally Consumed by Common People of Uttrakhand, India. IJBPAS. 2014;3(10):2389-2400.
Ohimain EI, Izah SC, Jenakumo N. Physicochemical and Microbial Screening of Palm Oil Mill Effluents for Amylase Production. Greener Journal of Biological Sciences. 2013;3(8):314 – 325.
Banerjee S, Sanjay KR, Chethan S, Malleshi NG. Finger millet (Eleusine coracana) Polyphenols: investigation of their antioxidant capacity and antimicrobial activity. African Journal of Food Science 2012;6(13):362-374. DOI: 10.5897/AJFS12.031
Flanagan M. The Physiology of Wound Healing. Review, Journal of Woundcare. 2000;9(6).
Carolyn AS, Malcolm WRR, Nicola JB. A critical analysis of current In vitro and In vivo angiogenesis assays. Int. J. Exp. Path. 2009;90:195 – 221.
Rupesh T, Nitika J, Raghvendra P, Sardul SS. Practices in Wound Healing Studies of Plants. Evidence-Based Complementary and Alternative Medicine. Hadawi Publishing Corporation. 2011; 2(11):1-17.
Barua C, Talukdar A, Begum SA. “Wound Healing Activity of Methanolic Extract of Leaves of Alternanthera brasiliana Kuntz using in vivo and in vitro Model. Indian Journal of Experimental Biology. 2009;47 (12):1001–1005.
Gupta A, Upadhyay NK, Sawhney RC, Kumar R. A poly-herbal formulation accelerates normal and impaired diabetic wound healing. Wound Repair and Regeneration. 2008;16 (6):784– 790.
Albaugh VL, Mukherjee K, Barbul A. Proline precursors and collagen synthesis: biochemical challenges of nutrient supplementation and wound healing. The Journal of nutrition. 2017;147(11): 2011-2017.
Fernandez DR, Vanderjagt DJ, Millson M, Huang YS, Chuang LT, Pastuszyn A, Glew RH. Fatty acid, amino acid, and trace mineral composition of Eleusine coracana (Pwana) seeds from northern Nigeria. Plant Foods for Human Nutrition. 2003;58(3):1-10.
Abstract View: 74 times
PDF Download: 32 times