Contraceptive Activity of Vitex doniana Stem Bark Methanol Extract in Female Albino Rats
International Journal of Biochemistry Research & Review,
Aims: The acceptability and accessibility of modern contraceptive drugs are limited especially in northern Nigeria. These contraceptives also have numerous side effects hence there is need to search for safe natural alternatives from medicinal plants. This research was aimed at evaluating the contraceptive effect of stem bark methanol extract of Vitex doniana in female albino rats.
Place and Duration of Study: Department of Biochemistry, Faculty of Life Science, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria. Between August 2019 to July 2020.
Methodology: Vitex doniana stem bark was extracted with methanol and the extract was subjected to qualitative phytochemical screening. Acute toxicity (LD50) of V. doniana stem bark extract was determined using up and down method and anti-fertility effect was evaluated via (anti-ovulation, anti-implantation and serum hormonal assay).
Results: The results for phytochemical screening showed the presence of alkaloids, flavonoids, tannins, steroids, glycoside, balsam and volatile oil. The LD50 of the extract was estimated to be greater than 5000 mg/kg as no mortality or any sign of toxicity are recorded within 14 days. The anti-fertility studies, methanol stem bark extract of Vitex doniana showed anti-ovulation activity through alteration of estrous cycle, changes in the histology of ovarian corpus luteum and decreasing number of follicles of extract treatment groups compared to control. Serum hormonal assay showed significant decrease (P<0.05) in oestrogen and progesterone level respectively in the extract treated groups compared to control group. Also, anti-implantation effect was observed in drug treated group (levenogesterel) and group treated with 400 mg/kg of V. doniana stem bark as there was no evidence of conception.
Conclusion: The present study revealed that methanol stem bark extract of Vitex doniana is relatively nontoxic at acute dose and possess a moderate amount of antifertility agent.
- hormonal assay
- vitex doniana
How to Cite
Johnston, Olwyn, et al. "Prevention of sepsis during the transition to dialysis may improve the survival of transplant failure patients." Journal of the American Society of Nephrology. 2007;18(4)1331-1337.
Adebisi, Iyabo Mobolawa, Shaibu Oricha Bello. "An ethnobotanical survey of herbal male contraceptives used in south-west Nigeria." African Journal of Pharmacy and Pharmacology. 2011;5(2)289-291.
Ogboghodo EO, Adam VY, Wagbatsoma VA. "Prevalence and determinants of contraceptive use among women of child-bearing age in a rural community in southern Nigeria." Journal of Community Medicine and Primary Health Care. 2017;29(2)97-107.
Daniyal Muhammad, Muhammad Akram. "Antifertility activity of medicinal plants." Journal of the Chinese Medical Association. 2015;78(7):382-388.
Berihun Tariku, Eyayu Molla. "Study on the diversity and use of wild edible plants in Bullen District Northwest Ethiopia." Journal of Botany; 2017.
Bongaarts John, Judith Bruce. "The causes of unmet need for contraception and the social content of services." Studies in Family Planning. 1995;57-75.
Dupont Éric, et al. "Antiangiogenic and antimetastatic properties of Neovastat (AE-941), an orally active extract derived from cartilage tissue." Clinical and Experimental Metastasis. 2002;19(2):145- 153.
Harborne Jeffrey B. "Phenolic compounds." Phytochemical methods. Springer, Dordrecht. 1973;33-88.
Trease GE, Evans WC. "Pharmacognsy. 11th edn. Brailliar Tiridel Can." Macmillian Publishers. 1989;0(5):10-15.
Sofowora A. African medicinal plants. University of Ife Press (Nig). 3rd Edition. 1993;21-30.
Londonkar Ramesh L, Hanumanatappa B. Nayaka. "Effect of ethanol extract of Portulaca oleracea L on ovulation and estrous cycle in female albino rats." Journal of Pharmacy Research. 2013;6(4):431-436.
Tafesse, Geremew, Yalemtsehay Mekonnen, Eyasu Makonnen. "In vivo and in vitro anti-fertility and anti-implantation properties of Leonotis ocymifolia in rats." African Journal of Traditional, Complementary and Alternative Medicines. 2005;2(2):103-112.
Uchendu CN, Kamalu TN, Asuzu IU. "A preliminary evaluation of antifertility activity of a triterpenoid glycoside (DSS) from Dalbergia saxatilis in female Wistar rats." Pharmacological Research. 2000;41(5):521-525.
Osunuga IO, Kareem FA, Akindele RA, Kukoyi BI, Taiwo EO, Inegbeneboh D. Antifertility effect of P-Alaxin in male adult wistar rats. Journal of Natural Science Research. 2016;5(9).
Sparg SGꎬ, Light ME, Van Staden J. "Biological activities and distribution of plant saponins." Journal of Ethnopharmacology. 2004;94(2-3):219-243.
Ansel Howard C., Nicholas G. Popovich, Loyd V. Allen. Pharmaceutical dosage forms and drug delivery systems. Lippincott Williams and Wilkins; 1995.
Auta T, Hassan AT. "Alteration in oestrus cycle and implantation in Mus musculus administered aqueous wood ash extract of Azadirachta indica (neem)." Asian Pacific Journal of Reproduction. 2016;5(3):188-192.
Thakur, Shweta, et al. "Effect of Carum carvi and Curcuma longa on hormonal and reproductive parameter of female rats." International Journal of Phytomedicine. 2009;1(1).
Smith, Susan M, Freeman ME, Neill JD. "The control of progesterone secretion during the estrous cycle and early pseudopregnancy in the rat: prolactin, gonadotropin and steroid levels associated with rescue of the corpus luteum of pseudopregnancy." Endocrinology. 1975;96(1):219-226.
Shivalingappa H, et al. "Effect of ethanol extract of Rivea hypocrateriformis on the estrous cycle of the rat." Journal of Ethnopharmacology. 2002;82(1):11-17.
Akpantah AO, et al. "Effects of Garcinia kola seed extract on ovulation, oestrous cycle and foetal development in cyclic female Sprague-Dawley rats." Nigerian Journal of Physiological Sciences. 2005;20(1):58-62.
Beim PY, Cohn KH, Santistevan A. U.S. Patent application No. 15/963,563; 2019.
Goyal AK. "Phytochemistry and in vitro studies on anti-fertility effect of Ficus religiosa fruits extract on uterine morphology of goat (Capra hircus)." Int J Drug Dev Res. 2014;6(2)141-158.
Welt Corrine, Alan Schneyer. "Inhibin, activin, and follistatin in ovarian physiology." The Ovary. Academic Press. 2019;95-105.
Scaramuzzi RJ, et al. "A model for follicle selection and the determination of ovulation rate in the ewe." Reproduction, Fertility and Development. 1993;5(5):459-478.
Heininger Kurt. "The mutagenesis-selection-cascade theory of sexual reproduction." 2013.
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