Study on Phytochemical Profile and Antioxidant Activity of Achyranthes aspera Whole Plant
International Journal of Biochemistry Research & Review,
This study aims to establish qualitative as well as quantitative phytochemical profiles and determine the free radical scavenging activity of phytochemical constituents of the entire plant of Achyranthes aspera Linn. The extraction of dried plant material was carried out by cold maceration with methanol followed by partitioning with ethyl acetate. Preliminary phytochemical screening of the plant was carried out by performing various chemical tests. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and Aluminum chloride methods, respectively. Antioxidant activity was studied using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The result of study revealed the presence of flavonoids, alkaloids, terpenoids, phytosterols, tannins, saponins, phenolic compounds, and carbohydrates. Total phenolic content, total flavonoids content, and antioxidant activity of the extract were found as 209.007 µg GA/mg, 17.59 µg QE/mg, and 25.12% (100 µg/mL), respectively. The study showed that plant could be a source of antioxidant compounds.
- Achyranthes aspera
- antioxidant activity
- ethyl acetate extract
How to Cite
Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in pharmacology. 2014;4:177.
Singh AG, Kumar A, Tewari DD. An ethnobotanical survey of medicinal plants used in Terai forest of western Nepal. Journal of ethnobiology and ethnomedicine. 2012;8(1):19.
Ghimire BK, Seong ES, Kim EH, Ghimeray AK, Yu CY, Ghimire BK. A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal. Journal of Medicinal Plants Research. 2011;5(10):1884-91.
Burlakoti C, Kunwar RM. Folk herbal medicines of Mahakali watershed Area, Nepal. Medicinal plants in Nepal: An anthology of contemporary research2008;187-93.
Gewali MB, Awale S. Aspects of traditional medicine in Nepal: Japan: Institute of Natural Medicine. University of Toyama; 2008.
Hillman JR. Society for Experimental Biology, Seminar Series: Volume 4, Isolation of Plant Growth Substances: CUP Archive; 1978.
Basyal D, Neupane A, Pandey D, Shiva P. Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L. Journal of Nepal Chemical Society. 2021;42:115-24.
Kokate C, Purohit A, Gokhale S. Pharmacognosy: Nirali prakashan Pune; 2008.
Harborne A. Phytochemical methods a guide to modern techniques of plant analysis: springer science & business media; 1998.
Evans WC, Evans D. Chapter 26 - Alkaloids. In: Evans WC, Evans D, editors. Trease and Evans' Pharmacognosy (Sixteenth Edition): W.B. Saunders; 2009;353-415.
Das BK, Al-Amin MM, Russel SM, Kabir S, Bhattacherjee R, Hannan JMA. Phytochemical Screening and Evaluation of Analgesic Activity of Oroxylum indicum. Indian J Pharm Sci. 2014;76(6):571-5.
Evans WC, Evans D. Chapter 19 - Hydrocarbons and derivatives. In: Evans WC, Evans D, editors. Trease and Evans' Pharmacognosy (Sixteenth Edition): W.B. Saunders; 2009;173-93.
Evans WC, Evans D. Chapter 21 - Phenols and phenolic glycosides. In: Evans WC, Evans D, editors. Trease and Evans' Pharmacognosy (Sixteenth Edition): W.B. Saunders; 2009;219-62.
Evans WC, Evans D. Chapter 20 - Carbohydrates. In: Evans WC, Evans D, editors. Trease and Evans' Pharmacognosy (Sixteenth Edition): W.B. Saunders; 2009;194-218.
Evans WC, Evans D. Chapter 18 - Basic metabolic pathways and the origin of secondary metabolites. In: Evans WC, Evans D, editors. Trease and Evans' Pharmacognosy (Sixteenth Edition): W.B. Saunders; 2009;148-67.
Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature protocols. 2007;2(4):875-7.
Baba SA, Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science. 2015;9(4):449-54.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry.1999; 64(4):555-9.
Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chemistry. 2012;130(4):1036-43.
Mensor LL, Menezes FS, Leitão GG, Reis AS, dos Santos TC, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research: PTR. 2001;15(2): 127-30.
Ahmed D, Khan MM, Saeed R. Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants (Basel, Switzerland). 2015;4(2):394- 409.
Matsuura HN, Fett-Neto AG. Plant Alkaloids: Main Features, Toxicity, and Mechanisms of Action. In: Carlini CR, Ligabue-Braun R, editors. Plant Toxins. Dordrecht: Springer Netherlands; 2017;243-61.
Dini I. 1 - An Overview of Functional Beverages. In: Grumezescu AM, Holban AM, editors. Functional and Medicinal Beverages: Academic Press; 2019;1- 40.
Veeru P, Kishor MP, Meenakshi M. Screening of medicinal plant extracts for antioxidant activity. Journal of Medicinal Plants Research. 2009;3(8):608-12.
Govindappa M, Bharath N, Shruthi HB, Santoyo G. In vitro Antioxidant Activity and Phytochemical Screening of Endophytic Extracts of Crotalaria pallida. Free Radicals and Antioxidants. 2011;1(3):79-86.
Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. African Journal of Biotechnology. 2008;7 (18):3188-92.
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