Ameliorative effect of Ethanol Extract of Annona Muricata Leaves in Sodium Arsenite Induced- Toxicity in Male Wistar Rats
International Journal of Biochemistry Research & Review,
Ingestion of arsenic, a known contaminant in drinking water causes cancer at multiple tissues and there is no cure. Consumption of arsenic contaminated water has been implicated metalloid-induced carcinogenesis. Research is therefore directed at chemoprevention using medicinal herbs for the management of arsenicosis. In this study hepatoprotective activity of ethanolic extract of Annona muricata (AM) leaves was assessed against sodium arsenite (SA) induced hepatic injury in albino rats. The animals were pre-treated with either 250 or 500mg/kg body weight of rat before exposure to SA. SA was dissolved in distilled water and administered at a dose of 5 mg/kg body weight on the 7th, 14th and 21st day of the experiment. SA was observed to induce a significant increase (p < 0.05) in serum aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase activities (ALP). However, pretreatments of rats with various doses of AM significantly (P<0.005) reduced serum enzyme levels to near normal against SA treated rats. Furthermore, histopathological observations revealed that treatment with AM extract protected the animals from SA induced liver damage. The results indicated that the leaves of Annona muricata possess hepatoprotective activity on SA induced hepatic injury in rats.
- Annona muricata
- sodium arsenite
- Oxidative stress
How to Cite
Chiou HY, Hsueh YM, Liaw KF, Horng SF, Chiang MH. Incidence of internal cancers and ingested inorganic arsenic: a seven-year follow-up study in Taiwan. Cancer Res. 1995;55:1296-1300.
Smith AH, Goycolea M, Haque R, Biggs ML. Marked increase in bladder and lung cancer mortality in a region of Northern Chile due to arsenic in drinking water. Am J Epidemiol. 1998;147:660-669.
Tsuda T, Babazono A, Yamamoto E, Kurumatani N, Mino Y, et al. Ingested arsenic and internal cancer: a historical cohort study followed for 33 years. Am J Epidemiol. 1995;141:198-209.
IARC (International Agency for Research on Cancer): Monographs on evaluation of carcinogenic risk to humans. In: Some Drinking Water Disinfectants and Contaminants, including Arsenic. 2004;84:269-477.
Chakraborti D, Sengupta MK, Rahman MM, Ahmed S, Chowdhury UK, Hossain MA, Mukherjee SC, Pati S, Saha KC, Dutta RN, Quamruzzaman Q: Groundwater arsenic contamination and its health effects in the Ganga-Meghna-Brahmaputra plain. J Environ Monit. 2004;6:74-83.
Gupta R, Kannan GM, Sharma M, Flora SJS. Therapeutic effects of Moringa oleifera on arsenic induced toxicity in rats. Environ. Toxicol. Pharmacol., 2005;20:456–464.
Tanju S, Madhuri D. Arsenic induced oxidative stress, hemato-biochemical and histological changes in liver and protective effect of Moringa leaf powder and ascorbic acid in broiler chicken. J. Chem. Pharm. Res. 2013;5:112-116.
Li X, Pi J, Li B, Xu Y, Jin Y, Sun G: Urinary arsenic speciation and its correlation with 8-0HDG in Chinese residents exposed to arsenic through coal burning. Bull Environ Contam Toxicol. 2008;81:406-411.
Cui X, Kobayashi Y, Hayakawa T, Hirano S: Arsenic speciation in bile and urine following oral and intravenous exposure to inorganic and organic arsenics in rats. Toxicol Sci. 2004;82:478-487.
Moore LE, Smith AH, Hopenhayn-Rich C, Biggs ML, Kalman DA, Smith MT. Decrease in bladder cell micronucleus prevalence after intervention to lower the concentration of arsenic in drinking water. Cancer Epidemiol Biomarkers Prev. 1997;6(12):1051-1056.
Mazumder DN. Effect of chronic intake of arsenic-contaminated water on liver. Toxicol Appl Pharmacol. 2005;206(2): 169-175.
Preston RJ, Dean BJ, Galloway S, Holden H, McFee AF, Shelby M. Mammalian in vivo cytogenetic assays. Analysis of chromosome aberrations in bone marrow cells. Mutat Res. 1987;189(2):157-165.
Kokate CK. Practical Pharmacognosy Vallabh Prakashan, New Delhi. 1994;107–113
Harborne JB. Phytochemical methods Chapman Hall, London. 1998;60–66
Reitman S, Frankel S. A calorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. clin. Pathol. 1957;28:56.
Kind P, King E. Estimation of plasma phosphatase by determination of hydrolysed phenol with aminoantipyrine. J. clin. Pathol., 1954;7:322.
Abernathy CO, Liu YP, Longfellow D, Aposhian HV, Beck B, Fowler B, Goyer R, Menzer R, Rossman T, Thompson C, Waalkes M. Arsenic: health effects, mechanisms of actions, and research issues. Environ Health Perspect. 1999;107(7):593-597.
Parvez F, Chen Y, Argos M, Hussain AZ, Momotaj H, Dhar R, van Geen A, Graziano JH, Ahsan H. Prevalence of arsenic exposure from drinking water and awareness of its health risks in a Bangladeshi population: results from a large population-based study. Environ Health Perspect. 2006;114(3):355-359.
Burak M, Imen Y. Flavonids and their antioxidants properties. Turkiye Klin Bil Derg. 1999;19:296-304.
Lambert JD, Hong J, Yang GY, Liao J, Yang CS. Inhibition of carcinogenesis by polyphenols: Evidence from laboratory investigations. Am J Clin Nutr. 2005;81(Suppl 1):284S-291S.
Okonkwo TJ, Okorie O, Okonta JM, Okonkwo CJ. Sub-chronic hepatotoxicity of Anacardium occidentale (Anacardiaceae) inner stem bark extract in rats. Indian J Pharm Sci. 2010;72:353-7.
Sharma A, Sharma MK, Kumar M. Modulatory role of Emblica officinalis fruit extract against arsenic induced oxidative stress in Swiss albino mice. Chem Biol Interact. 2009;180:20–30.
Roy S, Roy M, Pandey PK, Tiwari SP. Effects of tissue trace minerals status and histopathological changes in chronic arsenicosis in goats. Vet World. 2009;2(1):8–9.
Chattopadhyay S, Ghosh S, Debnath J, Ghosh D. Protection of sodium arsenite -induced ovarian toxicity by coadministration of L-ascorbate (Vitamin C) in mature strain rat. Arch Environ Contain Toxicol. 2001;41:83-9
Owumi SE, Odunola OA, Gbadegesin MA, Nulah KL. Protective effect of Juglans nigra on sodium arsenite-induced toxicity in rats. Pharmacognosy Research. 2013;5(3):183.
Lum G, Gambino SR. Serum gammaglutamyl transpeptidase activity as an indicator of disease of liver, pancreas, or bone. Clin Chem. 1972;18(4):358-362.
Meliker JR, Wahl RL, Cameron LL, Nriagu JO. Arsenic in drinking water and cerebrovascular disease, diabetes mellitus, and kidney disease in Michigan: a standardized mortality ratio analysis. Environ Health. 2007;2:4-6.
Odunola OA. Comparative effects of some local food condiments on sodium arsenite-induced clastogenicity. Afr J Med Med Sci. 2003;32(1):75-80.
Das NK, Sengupta SR. Arsenicosis: Diagnosis and treatment. India J Dermatol Venereol Leprol. 2008;74:571- 81
Thabrew M, Joice P. A comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Med. 1987;53:239–241.
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