Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

Main Article Content

Mahbuba Kaniz Hasna
Md. Abul Kashem
Farid Ahmed

Abstract

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment. 

Keywords:
Biological control, chickpea, soybean, Trichoderma.

Article Details

How to Cite
Hasna, M. K., Kashem, M. A., & Ahmed, F. (2020). Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea. International Journal of Biochemistry Research & Review, 29(9), 25-31. https://doi.org/10.9734/ijbcrr/2020/v29i930221
Section
Original Research Article

References

El-Hamidi M., Zaher FA. Production of vegetable oils in the world and in Egypt: an overview. Bulletin of the National Research Center. November. 2018;42(1)19:2-3.

Rahman MM, Hossain MM, Anwar MP, Juraimi AS. Plant density influence on yield and nutritional quality of soybean seed. Asian j. Plant Sci. 2011;10(2):125-132.

Suzuki F, Konno S. Regional report on grain legumes production in Asia. Tokyo, Japan. Asian Productivity Org. 1982;19-93.

El-Adawy TA. Nutritional compositions and antinutritional factors of chickpea (Cicer arietinum L.) undergo different cooking methods and germination. Plant Foods for Human Nutrition. 2002;57:83-97.

Ibrikci H, Knewtson SJB, Grusak MA. Chickpea leaves as a vegetable green for humans: evaluation of mineral composition. J. Sci. Food Agril. 2003;83: 945–950.

Nene YL, Sheila VK, Sharma SB. A world list of chickpea (Cicer arietinum L.) and pigeonpea (Cajanus cajan L.) pathogens. ICRISAT Pulse Pathology Progress Report 32. 1984;19.

Mahen VK, Mayee CD, Brenneman TB, Mcdonald D. Stem and pod rot of groundnut. Information Bulletin No. 44. ICRISAT, Patancheru 502324, India. 1995; 23.

Gurha SN, Dubey RS. Occurrence of possible source of resistance in chickpea (Cicer arietinum) against Sclerotium rolfsii Sacc. Madras Agriculturae J. 1982;70:63-63.

Mane SS, Khodke SW, Ghawade RS, Shrirao AV. Management of root and collar rot of soybean through biocontrol agent Trichoderma. Pl. Dis. Sci. 2013;8(1):86-91.

Okabe I, Morikawa C, Matsumoto N. Variation in southern blight fungus. In Japan detected by ITS-RFLP analysis JARQ. 2000;34:93-97.

Jegathambigai V, Wijeratnam W, Wijesundera, RLC. Effect of Trichoderma sp. on Sclerotium rolfsii, the causative agent of collar rot on Zamioculcas zamiifolia and an on farm method to mass produce Trichoderma species. Plant Pathol. J. 2010;9(2):47-55.

Sain SK, Pandey AK. Spectrum of three isolates of Trichoderma harzianum Rifai against important fungal diseases of tomato. 6th International Conference Plant Pathogens and People. New Delhi, India. 2016;23-27.

Banyal OK, Singh A. Integrated management of rajmash diseases in dry temperate region of north-western Himalayas. Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishva vidyalaya, Palampur 176062, India. Indian Phytopathology. 2007;60(3):317-321.

Papavizas GC. Trichoderma and Gliocladium: Biology, ecology and potential for biocontrol. Annu. Rev. Phytopathol. 1985;23:23.

Elad Y, Chet I, Katan J. Trichoderma harzianum: A biocontrol agent effective against Sclerotium rolfsii and Rhizoctonia solani. J. Phytopath. 1980;70:119-121.

Tuite J. Plant Pathological Methods: Fungi and bacteria. Burgess Pub. Co., Minneapolis; 1969.

Okereke VC. Control of the damping-off disease of tomato seedlings incited by Sclerotium rolfsii Sacc. using some plant extracts, captan and Trichoderma harzianum. M.Sc. Thesis, Michael Okpara University of Agriculture, Umudike Nigeria. 2004;23.

Asran-Amal A, Moustafa-Mahmoud SM, Sabet KK, El Banna OH. In vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in Trichoderma harzianum. Saudi J. Biol. Sci. 2010;17(2):153–157.

Begum M, Hossain MMI, Hoque MS. Biocontrol of seed borne Fusarium oxysporum with Trichoderma harzianum. Bangladesh J. Environ. Sci. 1998;4:128-133.

Bagwan NB. Evaluation of biocontrol potential of Trichoderma species against sclerotium rolfsii, Aspergillus niger and Aspergigillus flavus. Int. J. Plant Prot. 2011;4(1):107-111.

Kumar S. Trichoderma: a biological control weapon for managing plant diseases and promoting sustainability. Int. J. Agril. Sci. Vet. Med. 2013;1(3):106-121.

Elad Y, Rina B, Chat I. Parasitism of sclerotia of Sclerotium rolfsii by Trichoderma harzianum. Soil Biology and Biochemistry. 1984;16(4):381-386.

Khodke SW, Raut BT. Management of root/collar rot of soybean. Indian Phytopathol. 2010;63(3):293-301.

Kashem MA, Hossain I, Hasna MK. Use of Trichoderma in biological control of foot and root rot of lentil (Lens culinaris). Int. J. Sustain. Crop Product. 2011;6(1):29-35.

Kumar V, Shahid M, Srivastava M, Singh A, Pandey S, Sharma A. Enhancing seed germination and vigor of chickpea by using potential and effective strains of Trichoderma species. Virol. and Myco. 2014;3(2):1-3.

Shahid M, Singh A, Srivastava M, Sachan CP, Biswas SK. Effect of seed treatment on germination and vigour in chickpea. Trend in Biosci. 2011;4:205-207.

Entesari M, Sharifzadeh F, Ahmadzadeh M, Farhangfar M. Seed biopriming with Trichoderma species and Pseudomonas fluorescent on growth parameters, enzymes activity and nutritional status of soybean. Int. J. Agron. Plant Prod. 2013;4: 610-619.

John RP, Tyagi RD, Prévost D, Brar SK, Pouleur S, Surampalli RY. Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean. Crop Prot. 2019;29: 1452-1459.

Farid A, Hasna MK, Akter MB, Mondal MTR, Nabi, KME. Ecofriendly management of seedling diseases of chickpea (Cicer arietinum). Int. J. Biochem. Res. Rev. 2019;28(1):1-9.

Yadav J, Verma JP, Tiwari KN. Plant growth promoting activities of fungi and their effect on chickpea plant growth. AJBS. 2011;4:291-299.

Biswas KK, Das ND. Biological control of pigeon pea wilt caused by Fusarium udum with Trichoderma spp. Annals Plant Protection Sci. 1999;7:46-50.

Xu T, Zhong JP, Li DB. Antagonism of Trichoderma harzianum T82 and Trichoderma sp. NF9 against soil and seed borne fungal pathogens. Acta Phytopathol. Ca Sincia. 1993;23:63-67.

Prasad RD, Rangeshwaran R, Anuroop CP, Rashmi HJ. Biological control of wilt and root rot of chickpea under field condition. Ann. Plant Prot. Sci. 2002;10:72-75.