A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

Main Article Content

A. Ayisha Sireen
J. Anbumalarmathi


Abutilon indicum is consider to be used in the traditional system of medicine. It is found in tropical and subtropical regions of the world. It is used to treat various diseases. This plant does not cause any side effects to humans. As the plant has wide variety of medicinal properties, the present study aimed to comparative between plant and callus extract of Abutilon indicum (L.) sweet for antioxidant, antibacterial, antidiabetic and anti- proliferative activity. The highest percentage of callus induction (89.50%) and callus weight (1.26 g) was observed in T5 (MS + 2, 4-D (2.5 mg/l) + BAP (2 mg/l) and T8 [IBA (4 mg/l)] respectively. Phytochemical analysis of aqueous and ethyl acetate extracts of A. indicum in vivo plant and in vitro grown callus showed the presence of alkaloids, flavonoids, phenols, carbohydrates, glycosides, protein, terpenoids, saponins, tannins and coumarin. The total phenolic content was high in aqueous extract of callus (30.68 mg TAE/g). Maximum DPPH radical scavenging activity was found in aqueous extract of callus (86%) with IC50 value of 68.49 µg/ml. FT-IR analysis of aqueous extract of A. indicum plant and callus showed the presence of characteristic stretching at 2930.28 and 2927.75 indicating the presence of C-H stretching respectively. GC-MS analysis revealed the presence of 17 compounds in ethyl acetate plant extract, whereas 7 compounds in ethyl acetate callus extract such as tetradecane, 1-chloro, Sulfurous acid 2-prophytridecyl ester and 1- ethyl-3-[2-(octadecylthio) ethyl] thiourea. The ethyl acetate extracts of callus and plant and was found to be effective against Bacillus subtilis (3.1 mm) and Staphylococcus aureus (2.9 mm). Maximum α-amylase inhibitory activity was observed in aqueous callus extract (32.65%) with IC50 value of 833.61 µg/ml. HeLa cell viability was found to be 26.8% and 21.8% in plant and callus extract respectively.

Abutilon indicum, callus, phytochemicals, antioxidant, antidiabetic, cell viability.

Article Details

How to Cite
Sireen, A. A., & Anbumalarmathi, J. (2020). A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity. International Journal of Biochemistry Research & Review, 29(9), 13-24. https://doi.org/10.9734/ijbcrr/2020/v29i930220
Original Research Article


Joyti RR, Manorama M, Ritarani D, Santi Lata Sahoo. In vitro micropropagation of Abutilon indicum L. through leaf explant. Plant Tissue Culture and Biotech. 2009;19(2):177-184.

Rajalaxmi N, Ashok P, Luna Samanta, Santilata Sahoo. Phytochemical analysis of antioxidants from Abutilon indicum (L.) and Paederia foetida (L.) supported by TLC, FTIR and NMR studies. International Journal of Science and Research. 2013;4(5).

Sudarshana MS, Nissar AR, Grish HV. In vitro regeneration potentials of the medicinal plant Abutilon indicum (L.) Sweet. African Journal of Biotechnology. 2016;15(12):472-480.

Toshio Murashige, Folke Skoog. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-497.

Sujithra Devi R, Anbumalarmathi J, Aruna Sharmil S, Santhiya KR. Callus induction and micropropogation in Phyllanthus amarus. International Journal of Herbal Technology. 2016;1(1):23-26.

Ankita R, Kalpesh B. In vitro shoot multiplication from nodal explants of Coccinia grandis (L.) Voigt and its antidiabetic and antioxidant activity. Asian Journal of Biological Sciences. 2015;8:57-71.

Divya A, Anbumalarmathi J, Aruna Sharmili S. Phytochemical analysis, antimicrobial and antioxidant activity of Clitoria ternatea blue and white floweral leaves. Advances in Research. 2018;14(5):1-13.

Syed ZS, Krishna B, Kandukurivasu, Singara Charya MA. Antimicrobial activity of the fruit extracts of Coccinia indica. African Journal of Biotechnology. 2009;5(1):1-7.

Mercy Madhumitha K, Anbumalaramthi J, Aruna Sharmili S, Nandhini G, Shanmuga Priya. A comparative study of In vivo plant and In vitro callus extracts of Centratherum punctatum Cass. Annual Research & Review in Biology. 2020;35(3):1-13.

Nandhini G, Anbumalaramthi J, Mercy Madhumitha K, Shanmuga Priya G. A comparative study between plant and callus extracts: Analysis of phytochemical, antioxidant, antibacterial and anticancer properties of Ormocarpum sennoides (Wild.) DC. International Journal of Scientific Research in Biological Sciences. 2020;7(1):95-103.

Jayalakshmi V, Anbumalarmathi J, Arunasharmili S. In vitro callus induction of Coccinia indica (W. and A) and analysis of its phytochemical, antimicrobial, antioxidant and α- Amylase inhibitory activity- A comparative study. International Journal of Biochemistry Research & Review. 2019;28(3):1-16.

Chavan, Wadatkar CM. Isolation, purification and characterization of alpha amylase from Phaseolus aconitifolius. Journal of Advanced Drug Delivery. 2014;1(3):114-121.

Sen S, Jogeswar P. In vitro organogenesis of Abutilon indicum (L.) sweet from leaf derived callus and assessment of genetic fidelity using ISSR markers. The Journal of Horticulture Science and Biotechnology. 2018;94(1):70-79.

Surendra Kr Sharma, Naveen G. Preliminary phytochemical and pharmacognostic profile of Abutilon indicum Linn. root. Derpharmacia Letter. 2010;2(5):308-315.

Evanjaline R, Mohan VR. Screening of phytochemicals, in vitro antioxidant and antibacterial activites of stem and root extracts of Abutilon indicum (L.) SW. International Journal of Pharmaceutical Sciences Review and Research. 2017;43(1):75-83.

Srividya AR, Dhanabal SP, Jeevitha S, Vishnu Varthan VJ, Rajesh R. Relationship between antioxidant properties and chemical composition of Abutilon indicum Linn. Indian Journal of Pharmaceutical Science. 2012;74(2):163-167.

Saranya D, Sekar J. GCMS and FTIR analyses of ethyl acetate leaf extract of Abutilon indicum (L.) Sweet. International Journal of Advanced Research in Biological Sciences. 2016;3(2):193-197.

Sujatha, Rajesh GG, Chandra SK, Nagma K. Antibacterial activity of Abutilon indicum. World Journal of Pharmacy and Pharmaceutical Science. 2015;4(9):946-949.

Florenico Jr. V. Acre, Jeah E. Dela Concepcion, Katrina Mari C. Mayol, Gerard Lee L. See. In vitro α-amylase and α- glycosidase inhibition activity of Abutilon indicum (Linn 1836) root extracts. International Journal of Toxicological and Pharmacological Research. 2016;8(5):391-396.

Srikanth, Karthik, Sirisha, SasikanthChitti. Evaluation of antioxidant and anticancer properties of methanolic extracts of Abutilon indicum and Blumea mollis. Journal of Pharmacy Research. 2012;5(4): 2373-2376.