Main Article Content
Introduction: Hibiscus sabdariifa is a natural dye, generally called roselle. Most of Synthetic dye have been established to cause toxicity. The aim of this research was to determine the staining effect of different concentration of Hibiscus sabdariffa extracts at various pH, duration on fungal staining.
Methods: Standard Gomori’s metenaine silver stains as control and Hibiscus sabdariffa extracts (alcoholic and aqueous) were used to stain a fungal (Aspergillus spp) positive lung tissue, using various concentration (5% and 10%) of the extract, with change of pH achieved by treating the extracts with ammonium hydroxide and glacial acetic acid at various duration (30 seconds and 1 hour respectively), each was used for staining Aspergillus spp in tissue section as a primary stain replacing (4% Chromic acid, sodium metabisulphite, Hexamine working silver solution and Sodium thiosulphate).
Results: All extracts after treatment were acidic, the fungi were best demonstrated with Aqueous hibiscus stains (5% untreated, 10% untreated and 5% glacial acetic acid treated) at longer duration, staining the capsule, light to dark-brown in a light-green background when compared with the alcoholic hibiscus stain. Few of the alcoholic hibiscus stains show metachromatic property. The Hibiscus stains stained better at longer duration and the change of pH was indirectly proportional to the staining ability of the hibiscus stains.
Conclusion: The results obtained indicate that hibiscus plant extract have the potential for use in the morphological identification of fungi in formalin-fixed paraffin-embedded tissue section.
Kodrikar P. Probems and aspects of natural dyes [internet]; 2018.
(Accessed March, 2018)
Avwioro OG, Aloamaka PC, Ojianya NU, Oduola T, Ekpo OE. Extracts of pterocarpus osun as a histological stain for collagen fibres. Afr. J. Biotechnology. 2005;4:460-462.
Avwioro OG. Histochemistry and tissue pathology. Delta: Claverianum Press. 2010;134-260.
Baker FJ, Silverton RE and Pallister CJ. Baker and Silverton's introduction to medical Laboratory Technology. Nigeria: Bounty Press Limited. 2001;220-242:385-390.
Ochei J, Kolhatkar A. Medical laboratory science theory and practice. India, New Delhi. Tata McGraw-Hill Publishing Company Ltd. 2000;471-479.
Grifoni D, Bacci L, Zipoli G, et al. The role of natural dyes in the UV protection of fabrics made of vegetable fibres. Dyes Pigm. 2011;91:279-85.
Rubia, Bharwaj A. A review: Natural colourants. Int. J. Eng. Sci. Res. Technol. 2016;5:778-781.
Chinyere CG, Etoforinini CC. Alternative staining of kidney tissue sections with extracts of tumeric (Carcuma longa) and Zobo (Hisbiscus sabdariffa). J. Histotechnol. 2017;1-9.
Morton JF. Fruits of warm climates. Florida Flair Books; 1987.
Smith NM. Weeds of the wet/dry tropics of Austalia - A field guide. Enviroment Center NT, Inc. 2002;112.
Sridhara SU, Raju S, Gopalkrishna AH, et al. Hibiscus: A different Hue in Histo-pathology. J. Med. Rad. Path. Sur. 2016; 2:9-11.
Ola MA, Abiola OO, Benard SA, et al. Hibiscus-Shorgum: A new morphological stain in neuro-histology. Int. J. Health Res. Inno. 2016;4(1):31-38.
Deepali K, Laleta S, Deepika M. Application of aqueous plant extract as biological stains. IJSER. 2014;5(2):1586-1589.
Omorodion NT, Achukwu P. Investigation of Hibiscus sabdariffa (Roselle) as Histological stain and in assessment of bar bodies. Am. J. Biomed. Sci. 2017;9(1):15-19.
Cheng CW, Saad SM, Abdullah R. Alternative staining using extracts from Hibiscus (Hibiscus rosa-sinensis L.) and red beet (Beta vulgaris L.) in diagnosing ova of intestinal nematodes (Trichuris triciura and Ascaris lumbricoides). European J. Biotechnol. Biosci. 2014;1(5): 14-18.
Hashim EA. The use of watery extract of kujarat flower Hibiscus sabdariffa as a natural Histological stain. Iraqi J. Med. Sci. 2006;5(1):29-33.
Ihuma JO, Asenge GH, Abioye JOK, Dick SK. Application of methanolic extracts from Hibiscus sabdariffa Linn as a biological staining agent for some fungal species. IJPAES. 2012;2(2): 254-259.
Ibnouf A, AbdulRaheem E, Seed Ahmed M, Dahab D. Assessment of staining quality of Roselle (Hibiscus sabdariffa) on formalin fixed paraffin-embedded renal tissue sections. Int. J. Curr. Res. Rev. 2014;6(21):26-28.
Raheem EMA, Ibnouf AO, Shingeray OH, Farh HJM. Using of Hibiscus sabdariffa extract as a natural histological stain of skin. Am. J. Res. Commun. 2015;3(5):211-216.