Effect of Methanol Extract of Unripe Carica papaya Pulp on Lipid Profile and Liver Function of Alloxan-Induced Diabetic Rats

Main Article Content

Henry N. Ogbonna
Ugonna D. Nwankpa
Godwin S. Aloh
Raymond C. Ibeh


Background: Carica papaya is commonly used in the treatment of various diseases like constipation, piles, hypertension and malaria. The ameliorative effect of Carica papaya on alloxan-induced diabetic rats has been suggested by several studies.

Aim: The present study aims to investigate the effect of methanolic extract of unripe Carica papaya pulp on the lipid profile and liver biomarkers of a diabetic rat.

Materials and Methods: After acclimatization, thirty-two (32) animals were randomly classified into 8 groups (4 rats per group). The different test (extract) groups received in addition to normal diet ad libitum, dosages of 200, 400, 600 and 1000 mg/kg/day of the Carica papaya extract. The normal and negative control groups received a normal diet, while the positive control was given oral treatment with 6 mg/65 kg/day of glibenclamide as well as the normal diet. After 28 successive days of treatment, all the experimental rats were sacrificed by ocular puncture and the serum used in the evaluation of body lipids, liver function parameters and biochemical indexes.

Results: The administration of the unripe Carica papaya extract resulted in a dose-dependent and significant decrease (p<0.05) in serum albumin, aspartate aminotransferase (AST), total protein, and in alanine aminotransferase (ALT) level.  There was, however, a significant increase in High-density lipoprotein (HDL) level for 400-1000 mg/kg extract groups. The result also showed a reduced cholesterol level at an extract dose of 1000 mg/kg/day.

Conclusion: The ameliorative properties of the unripe pulp of Carica Papayaon biochemical parameters of a diabetic rat, as shown from the result could be indicative that unripe Carica papaya can be valuable in the management of diabetes mellitus and other complications that may arise.

Alloxan, Carica papaya, glibenclamide, liver function, hyperlipidemia, bilirubin

Article Details

How to Cite
Ogbonna, H. N., Nwankpa, U. D., Aloh, G. S., & Ibeh, R. C. (2020). Effect of Methanol Extract of Unripe Carica papaya Pulp on Lipid Profile and Liver Function of Alloxan-Induced Diabetic Rats. International Journal of Biochemistry Research & Review, 29(4), 1-11. https://doi.org/10.9734/ijbcrr/2020/v29i430180
Original Research Article


Ime FA, Atangwho IJ, Regina I et al. Hypoglycemic effect and proximate composition of some selected Nigerian traditional diets used in management of diabetes mellitus. Eur J Food Res Rev. 2011;1(2):94–101.

Anuradha G, Malini S, Jyoti S. A role of insulin in different types of diabetes. Int J Curr Microbiol App Sci. 2015;4(1):58-57.

Scott AR, Clarke BE, Healy H et al. Microvascular complications in cystic fibrosis – related diabetes mellitus; a case report. Pancreas. 2000;1(4):208-210.

Chatila R, West AB. Hepatomegaly and abnormal liver tests due to glycogenesis in adults with diabetes. Medicine. 1996;75: 327–333.

Lenzen S, Panle V. Alloxan; history and mechanism of action. Diabetologia. 1988; 31:337- 342.

Aloulou A, Hamden K, Elloumi D et al. Hypoglycemic and anti-lipidemic properties of kombucha tea in alloxan-induced diabetic rats. BMC Complement Altern Med. 2012;12:63.

Abayomi S, Eyitope O, Adedeji O. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013;10(5): 210–229.

Okpe O, Ibrahim S, Njoku GC, et al. Pancreatic islet regeneration and some liver biochemical parameters of leaf extracts of Vitex doniana in normal and streptozotocin-induced diabetic albino rats. Asian Pac J Trop Biomed. 2014;4(2):124-130.

Adeneye AA, Olagunju JA. Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn. in Wistar rats. MOJ Biology and Medicine. 2009;1(1):1-10.

Venkateshwarlu E, Dileep P, Rakesh KR, et al. Evaluation of antidiabetic activity of Carica papaya seeds on streptozotocin- induced type-II diabetic rats. Journal of Advanced Applied Scientific Research. 2013;4(2):38–41.

Juárez-Rojop IE, Díaz-Zagoya JC, Ble-Castillo JL, et al. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2012;12:236.

Nwangwa EK, Ekhoye EI. Anti-hyperlipidemic activity of aqueous extract of Carica papaya seed in albino rats fed with high fat diet. Curr Trends Tech Sci. 2013;2(3):262-6.

Arun KS, Vinay KS. Carica papaya- A herbal medicine. Int J Res Stud Biosci. 2016;4(11):19-25.

Daniele S, Luca R, Gian P, et al. Sulfonylureas and their use in clinical practice. Arch Med Sci. 2015;11(4):840-848.

Bolkent S, Yamardag R, Tabakogluoguz A et al. Effects of chord (Beta vulgaris L. Var. cicla) extract on pancreatic β-cells in Streptozotocin-diabetic rats: A morphologic and biochemical study. J. Ethnopharmacol. 2000;73:251–259.

Changting X, Mirjana Pavlic, Linda S, et al. Effects of acute hyperglucagonemia on hepatic and intestinal lipoprotein production and clearance in healthy humans. ‎Diabetes Care. 2012;60(2):383-390.

Zetina-Esquivel AM, Carlos AT, Crystell GG, et al. Effect of Carica papaya leaf extract on serum lipids and liver metabolic parameters of rats fed a high cholesterol diet. SCIRP. 2015;7:1196-205.

Rao AV. Role of dietary sources of saponins in chemotherapy strategy of lowering the risk of human cancers. Univ. Tor. 1993;219-225.

Owoyele BV, Yakubu MT, Alonge F et al.Effects of folic acid intake on serum lipid profile on apparently healthy young adult male Nigerians. Afr. J. Biomed. Res. 2005; 8:139-142.

Brain J, Welch MD, Ivana Z. Diabetic ketoacidosis in Type 2 Diabetes. J Diabetes Res. 2004;22(4):198–200.

El-Demerdash FM, Yousef MI, Abou-El-Naga NI. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food Chem. Toxicol. 2005;43:57–63.

Recknagel RO, Glende EA, Dolak JA, Waller RL. Mechanisms of carbon tetrachloride toxicity. Pharmacol. Ther. 1989;43(1):139-154.

Emang E, Helal NA, ALsayeda MK, Mohammad AZ. Anti-diabetic effect of artemisia annua (Kaysom) in alloxan-induced diabetic rats. Egypt. J. Hosp. Med. 2014;57:422-430.

Sivajothi V, Dey AB, Jayakar BR. Antihyperglycemic, antihyperlipidemic and antioxidant effect of Phyllanthus rheedii on streptozotocin induced diabetic rats. Iran J Pharm Res. 2008;7(1): 53-59.