Hop-derived Xanthohumol Induces HL-60 Leukemia Cells Death

Main Article Content

M. Pacurari
H. Brown
A. Rieland

Abstract

Background: Acute promyelocytic leukemia (APL) affects both kids and adults, however it is more prevalent in younger population. Although APL has a favorable prognostic, patients that relapse often do not respond positively to additional chemotherapy. Therefore, there is a need to further identify ways to overcome these challenges. 

Hypothesis: In this study, we examined antileukemic effects of xanthohumol (XN), a prenylated flavonoid derived from hops (Humulus lupulus L), on human promyelocytic HL-60 cells. 

Materials and Methods: HL-60 cells were exposed to different concentrations of XN (μM) for 24 h. Cell viability, cell morphology, chromatin condensation, cPARP-1 level, and caspase-3 activation, and the expression of p21WAF1/Cip1 were analyzed.

Results: XN reduced HL-60 cell viability in a dose-dependent manner. XN induced a dose-dependent morphological changes including cell shrinkage and blebbing, and significantly increased the number of cells with condensed chromatin. XN significantly increased the level of cPARP-1, active caspase-3, and the expression of p21WAF/CIP mRNA.

Conclusion: These data indicate that XN induces HL-60 cell death by regulating cell cycle progression and apoptosis. This study suggests that XN may have antileukemic preventive effects.

Keywords:
Acute promyelocytic leukemia, apoptosis, caspase-3, p21, xanthohumol, plant derived, HL-60 cells.

Article Details

How to Cite
Pacurari, M., Brown, H., & Rieland, A. (2020). Hop-derived Xanthohumol Induces HL-60 Leukemia Cells Death. International Journal of Biochemistry Research & Review, 29(1), 61-72. https://doi.org/10.9734/ijbcrr/2020/v29i130165
Section
Original Research Article

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