Effects of Repeated Administration of Extracts from Arachis hypogaea Hulls on Blood Parameters and Histological Organization of Heart, Liver and Kidneys of Rats

Main Article Content

Djyh Bernard Nazaire
Tra Bi Irié Otis
Oungbé Monkoué Désiré
Gnahoué Goueh Laurent
Kra Adou Koffi Mathieu
D’almeida Marie-Anne Kayi
Djaman Allico Joseph

Abstract

Aims: Arachis hypogaea (peanuts) is widely used in food worldwide. Therapeutic use of various parts of this plant has been mentioned in many traditional medicinal systems. The aim of the present study was to evaluate subacute toxicity of methanolic and aqueous extracts of peanuts hulls.

Place and Duration: Laboratory of Biochemical Pharmacodynamics, Faculty of Biosciences, University Félix Houphouët-Boigny, from March to June 2018.

Methodology: Serial extraction was done using methanol and water as solvents. The study was evaluated by orally daily doses of extracts 300, 600 and 1200 mg/kg. The treatment for 28 days concerned seven groups of animals, the control group and six treated groups. Each group included six animals, 3 males and 3 females. Animals of control group didn’t have any treatment. Animals were observed for general behavioural and signs of abnormalities during the experiment. After all treatments, blood was collected for haematological and biochemical analysis. Liver, kidney, and heart were removed, weighed for histological study.

Results: The results showed that, there were not any significant (p˃0.05) changes in both the absolute and relative organ weights between the control and the test groups. Biochemical parameters were statistically equal in all groups. In addition, both extracts did not induce any significant effect on RBC and indices relating to it (HGB, PCV, MCV, MCH and MCHC) throughout the experimental period. But, there was a decrease (16.33±1.68) on WBC with methanolic extract compared to control (13.79±2.73). Histological examination of the liver, kidneys, and the heart showed normal organisation and structure of heart, kidneys and liver.

Conclusion: It appears that the methanolic and aqueous extracts of hull of Arachis hypogaea did not produce any toxicity in oral subacute toxicity study. However, further studies are needed to confirm long term toxicities.

Keywords:
Subacute, Arachis hypogaea hulls, haematological, blood parameters, histological study.

Article Details

How to Cite
Nazaire, D. B., Otis, T. B. I., Désiré, O. M., Laurent, G. G., Mathieu, K. A. K., Kayi, D. M.-A., & Joseph, D. A. (2020). Effects of Repeated Administration of Extracts from Arachis hypogaea Hulls on Blood Parameters and Histological Organization of Heart, Liver and Kidneys of Rats. International Journal of Biochemistry Research & Review, 29(1), 20-33. https://doi.org/10.9734/ijbcrr/2020/v29i130162
Section
Original Research Article

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