Antidiabetic and Modulatory Effect of Ethanol Extract of Neem Leaf on Some Essential Biochemical Parameters of Streptozotocin-induced Diabetic Rats

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Ezeigwe Obiajulu Christian
Ezeonu Francis Chukwuemeka


The study was conducted to investigate the modulatory activity of ethanol extract of neem leaf on the essential biochemical parameters of streptozotocin-induced diabetic rats. The 24-hour acute toxicity test of the orally administered ethanol extract was determined using Finney’s method. Diabetes was induced in the rats by a single intraperitoneal dose of 50 mg/kgbw of streptozotocin (STZ). Blood was collected after 28 days of treatment and used for biochemical analysis. Biochemical analysis was done using standard biochemical methods. The acute toxicity study of the ethanol extract reveal that the median lethal dose was 5.0 g/kg body weight. The anti-diabetic study carried out for a period of 28 days showed a significant (p<0.05) reduction in the fasting blood glucose levels of the animals treated with ethanol extract compared with the diabetic untreated rats. The urea, creatinine, ALT, AST, ALP and bilirubin levels increased significantly (p<0.05) in the diabetic untreated rats compared with the groups treated with the ethanol extract of neem leaf. The serum alpha-amylase activity of the treatment groups decreased significantly (p<0.05) compared with the diabetic untreated group. The diabetic untreated rats showed a significant (p<0.05) decrease in their insulin level compared with the insulin levels of animals treated with the ethanol extract of neem leaf. The ethanol extract of neem leaf is efficacious in lowering blood glucose level in STZ-induced diabetic rats by regenerating the beta cells integrity which was seen from the increase in the insulin level of the extract treated rats. It modulates the essential biochemical parameters of diabetic rats favourably towards recovery and improved health.

Neem, leaf, ethanol, extract, biochemical, streptozotocin.

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Obiajulu Christian, E., & Chukwuemeka, E. F. (2020). Antidiabetic and Modulatory Effect of Ethanol Extract of Neem Leaf on Some Essential Biochemical Parameters of Streptozotocin-induced Diabetic Rats. International Journal of Biochemistry Research & Review, 28(4), 1-11.
Original Research Article


Quinn CH, Royak-Schaler R, Dan Lender D, Steinle N, Gadalla SH, Zhan M. Patient understanding of diabetes self-management: Participatory decision-making in diabetes care. Journal of Diabetes Science and Technology. 2011;5 (3):723-730.

Marina K, Maria K. Education and diabetes mellitus. Health Science Journal. 2012;6:1-9.

Chinenye S, Uloko AE, Ogbera AO, Ofoegbu EN, Fasanmade OA, Fasanmade AA, Ogbu OO. Profile of Nigerians with diabetes mellitus – Diabcare Nigeria study group: Results of a multicenter study. Indian Journal of Endocrinologyand Metabolism. 2012;16(4):558–564.

Rother KI. Diabetes Treatment Bridging the Divide. New England Journal of Medicine. 2007;356 (15):1499-1501.

Steinsbekk A, Rygg L, Lisulo M, Rise M, Fretheim A. Group based diabetes self-management education compared with routine treatment for people with type 2 diabetes mellitus. A systematic review with meta-analysis. BMC Health Services Research. 2012;12:213.

Horvath K, Koch K, Jeitler K. Effects of treatment in women with gestational diabetes mellitus: Systematic review and meta-analysis. British Medical Journal. 2010;340:c1395.

Lawrence JM, Contreras R, Chem W, Sacks DA. Trends in the Prevalence of Preexisting Diabetes Mellitus and Gestational Diabetes Mellitus. Diabetes Care. 2008;31(5):899-904.

Selvin E, Steffes MW, Zhu H. Glycated hemoglobin, diabetes, and cardiovascular risk in nondiabetic adults. New England Journalof Medicine. 2010;362:800–811.

Tobias DK, Hu FB, Chavarro J, Rosner B, Mozaffarian D, Zhang C. Healthful dietary patterns and type 2 diabetes mellitus risk among women with a history of gestational diabetes mellitus. Archivesof Internal Medicine. 2012;172:1566–1572.

Wu AY, Kong NC, de-Leon FA. MAPS Investigators. An alarmingly high prevalence of diabetic nephropathy in Asian type 2 diabetic patients: the MicroAlbuminuria Prevalence (MAP) Study. Diabetologia. 2005;48:17–26.

Kempen JH, O’Colmain BJ, Leske MC. Eye diseases prevalence research group. The prevalence of diabetic retinopathy among adults in the United States. Archives of Ophthalmology. 21004;122: 552–63.

Christian EO, Felicia EC, Helen IN, Soronnadi Vivian Nneka, Chukwuemeka Ugochi Vivian, Alaebo Prince Ogochukwu, Antidiabetic property and antioxidant potentials of aqueous extract of Azadirachta indica leaves in streptozotocin-induced diabetic rats. Journal of Medicinal Plants Studies. 2019; 7(6):18-23.

Neli A. Diabetes and cardiovascular disease. Diabetes, Obesity Metabolism. 2003;5(1):11-18.

Adejumo OP, Adeniyi FA, Fasanmade AA. Results of a 60 second foot screening for patients with diabetes conducted on the 2011 world diabetes day. JournalNursing Eduation Practice. 2012;3(9):1–9.

Ladeji O, Omekarah I, Solomon M. Hypoglycemic Properties of Aqueous Bark Extract of Oeibapentandra in Streptozotocin-induced Diabetic Rats. Journal Ethnopharmacology. 2003;84: 139-142.

Agunu A, Sadiq Y, Gabriel OA, Zezi AU, Abdurrahman EM. Evaluation of five Nigeria medicinal plants used in treatment of diarrhea in Nigeria. Journal of Ethnopharmacology. 2005;61:209- 213.

Khosla PS, Bhanwra J, Singh S, Srivasta RK. A study of hypoglycaemic effects of Azadirachta indica (Neem) in Normaland Alloxan Diabetic Rabbits. Indian Journal of Physiology and Pharmacology. 2005;44: 69-74.

Rakieten N, Rakieten ML, Nadkarni MV. Studies on the Diabetogenic Action of Streptozotocin NSC-37917). Cancer Chemistry and Reproduction. 1963;29:91–102.

Trinder P. Determination of Glucose in Blood using Glucose Oxidase with an Alternative Oxygen Acceptor”. Annals of Clinical Biochemistry. 1972;6:224-227.

Finney DJ. New York-London: Cambridge Univ. Press. 2nd Ed. Science. 1952;116 (3011):286-287.

Raizada RB, Srivastava MK, Kaushal RA, Singh RP. Azadirachtin, a neem biopesticide: Subchronic toxicity assessment in rats. Food and Chemical Toxicology. 2001;39(5):477–483.

Lorke D. A new approach to practical acute toxicity testing. Archives of Toxicology. 1983;54:275–87.

Shravan KM, Kannappan N. Invivo Antidiabetic Evaluation of Neem Leaf Extract in Alloxan Induced Rats. Journal of Applied Pharmaceutical. Sciences. 2011;7: 100-105.

Mohammed A, Abubaka SA. Potency of Aqueous Extract of Azadirachta indica. Juss against Lipid Peroxidation and Liver Damage in Rats. International Journal of Modern Biochemistry. 2012;1 (1): 27-35.

Zohre MD, Forogh N, Mohamad EA. Alpha amylase Inhibition Activity of Some Plant Extract of Teucium species. European Journal of Biological Sciences. 2015;7(1): 26-31.

Kazeem MF, Dansu TV, Adeola SA. Inhibitory effect of Azadirachta indica A. Juss Leaf extract on the activities of α-amylase and α-glucosidase. Pakistan Journal of Biological Science. 2013;16 (21):1358-62.

Shukia R, Sharma SB, Puri D, Prabhu KD, Murthy PS. Medicinal plants for treatment of diabetes mellitus. Indian Journal of Clinical Biochemistry. 2000;15(1): 169-177.