Effects of Aqueous Extract of Ocimum gratissimum Leaves and Vitamin C on Lead Acetate-induced Changes in the Thymus of Adult Wistar Rats
International Journal of Biochemistry Research & Review,
Lead is a heavy metal found in earth’s crust. It is a widespread and insidious environmental toxin known as a severe and aggressive contaminant to human and animal organisms’ health status. This work is aimed at evaluating the effect of aqueous extract of Ocimum gratissimum (OG) on lead induced changes in the thymus of adult albino wistar rats. Thirty five male Wistar rats were used in the study and were randomly divided into seven groups with five rats in each group. The rats in Group one (G1) served as the Control and received distilled water. Group 2 received 120 mg/kg body weight (bwt) of lead acetate, G3 received 375 mg/kg bwt of OG only. G4 received 120 mg/kg bwt of lead acetate and OG extract at 375 mg/kg bwt., G5 received 120 mg/kg bwt of lead and OG at 750 mg/kg bwt. G6 received 375 mg/kg bwt of OG in two weeks followed by 120 mg/kg bwt of lead acetate for one week, while G7 received 120 mg/kg bwt of lead acetate and Vitamin C at 11900 mg/kg. All the administrations were carried out orally for twenty one (21) days. At the end of the administration, the rats were fasted for 24 hours. They weighted and humanely sacrificed via cervical dislocation. The thymus were harvested in all the groups and prepared for histological studies using routine haematoxylin and eosin (H&E) staining techniques. The result of the present study shows that lead possessed a distortive effect in the histoarchitecture of thymus of the wistar rat in G2 when compared with G1. Treatment of these experimental animals with vitamin C prove to posses more ameliorative effect in restoring the histoarchitecture on lead toxicity in the thymus closed to normal than OG. Therefore, it is suggested that aqueous extract OG can act via the same pathway as vitamin C, in maintaining the normal histological structures of the thymus of adult albino wistar rat exposed to lead toxicity.
- Ocimum gratissimum
- Vitamin C
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Atlanta Georgia. Toxicological profile for cadmium. ATSDR, agence for toxic substance and disease registry; 1998.
Watemaux C, et al. Methodological isseus in modeling the relationship between low – level lead exposure and infant develop-ment: Examples from the boston lead study. Environ Res. 1985;38:119-129.
Sharma RP, Street JC. Public health aspects of toxic heavy metals in animal feeds. J Am Vet Med Assoc. 1980;177: 149-153.
Bergendi L, Benes L, Durackova Z, Ferenci KM. Chemistry, physiology and pathology of free radicals. Life Science. 1999;65:1865-1874.
Gurer H, Ozygunes H, Saygin E, Ercal N. Antioxidant effect of Taurine against lead - induced oxidative stress. Arch. Environ. Contam. Toxicol. 2001;41:397-402.
Simon JA. Relationship of ascorbic acid to blood leads levels. JAMA. 1999;281:2289-2293.
Hudes ES. Relationship of ascorbic acid to blood leads levels. JAMA. 1999;281:2289-2293.
Vierra RF. Chemical characterization of Ocimum gratissimum found in the market and used in Traditional medicine in Brazil. Journal of Economic Botany. 2000;20:5-6.
Simon JE. Chemical characterization of Ocimum gratissimum found in the market and used in Traditional medicine in Brazil. Journal of Economic Botany. 2000;20:5-6.
Owulade MO. Handbook African medicinal plants. CRC Press, Boca Raton, Florida, USA. 2004;214-215.
Iwu MM. Handbook of African medicinal plants. CRC Press, New York. 1993;214-215.
Nakamura CV, Ishid K, Faccin LC, Filho BP, Cortez DA, Rozental S, Souza W, Ueda N. In vitro activities of essential oils from Ocimum gratissimum against four Candida species. Research in Micro-biology. 2004;155(7):579-86.
Lymphoid organs; 2018.
Available:http://teachmeseries.com (Assessed 23 June 2018)
Odey MO, Mboso O. Nat prod leaves were blended into fine powder using electric blender model QBL -18L40. Biotechnology Advances J. Biotech; 2012.
Ebeye OA, Ekundina OV, Wilkie IE. Histological and biochemical effects of aqueous extract of Ocimum gratissimum on the liver and kidney of adult wistar rats, African Journal of Cellular Pathology. 2014;2:59-64.
Ofem OE, Ani EJ, Eno AE. Effect of aqueous leaves extract of Ocimum gratissimum on hematological parameters in rats. International Journal of Applied Asic Medical Research. 2012;2(1):38–42.
Material Safety Data Sheet (MSDS). Safety data for mercuric chloride physical and chemical properties. Janssen Pharmaceutical 3a. 2440 Geel, Belgium; 2008.
Sheehan DC, Hrapchak BB. Theory and practise of histotechnology. Battelle Memorial Institue; 1987.
Eisler R. Lead hazards to fish, wildlife, and invertebrates, asynoptic review, Biological Report 85(1.14), Contaminant Hazard Reviews 14, Laurel, MD, USA: US Fish and Wildlife Service, 1988 (Last modified in 2013); 1988.
Nwokocha CR, Owu DU, Ufearo CS, Iwuala MO. Comparative study on the efficacy of Garcinia kola in reducing some heavy metal accumulation in liver of Wistar rats. Journal of Ethnopharmaco-logy. 2011;135:488-91.
Klaassen CD. Heavy metals and heavy-metal antagonists In: Goodman and Gilman’s Pharmacological Basis of Therapeutics. (10th edition.), McGraw-Hill, New York; 2001.
Yeh YC, Liu TJ, Wang LC, Lee HW, Ting CT. A standardized extract of Ginkgo bilobasuppresses doxorubicin-induced oxidative stress and p53- Mediated mitochondrial apoptosis in at testes. British Journal of Pharmacology. 2009; 156:48-61.
Buck M, Chojkier M. Muscle wasting and dedifferentiation induced by oxidative stress in a murine model of cachexia is prevented by inhibitors of nitric oxide synthesis and antioxidants. EMBO Journal. 1996;15:1753-1765.
Silbergeld EK, Waalkes M, Rice JM. Lead as carcinogen: Experimental evidence and mechanism of action. Am. J. Indus. Med. 2000;38:316-323.
Patrick L. Lead toxicity part II. The role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern. Med. Rev. 2006;11:114-127.
Jabeen R, Tahir M, Raza SMH, Nisa FU, Batool S. Protective role of vitamin C against teratogenic effects of lead acetate on mouse kidney. 2011;1-5.
Raafat BM, El-Barbary A, Touson E, Aziz S. Di-mercapto siccinic acid (DMSA) and vitamin C chelating potenctin lead intoxication, regarding oxidative stress and apoptoic related proteins in rabblits. Jornal of Genetic Enginerring and Biotechnology. 2011;9(2):121-131.
Hamadouche Slimani M, Aoues A. Beneficial effect of administration of vitamin C in amelioration of lead hepato-toxicity. Nat. Sci. Biol. 2012;4(3):7-13.
Dewanjee S, Sahu R, Karmakar S, Gangopadhyay M. Toxic effects of lead exposure in Wistar rats: Involvement of oxidative stress and the beneficial role of edible jute (Corchorus olitorius) leaves. Food Chem. Toxicol. 2013;55:78-91.
Bashandy SA. Beneficial effect of combined administration of vitamin E in amelioration of chronic lead hepatotoxicity. Egyp J Hosp Med. 2006;23:371-84.
Haleagrahara T, Jackie S, Chakravarthi M, Rao M, Kulur A. Protective effect of Etlingera eletior (torch ginger) extract on lead acetate-induced hepatotoxicity in rats. J. Toxicol. Sci. 2010;35(5):663-671.
El-Neweshy MS, El-Sayed YS. Influence of vitamin C supplementation on lead-induced histopathological alterations in male rats. J. Exp. Toxicol. Pathol. 2011; 63:221-227.
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