Effect of Enzyme Replacement Therapy on Disease Burden Biomarkers in Gaucher's Disease
Mohammed H. Hassan *
Department of Medical Biochemistry and Molecular Biology, Qena Faculty of Medicine, South Valley University, P.O.Box No. 83523 Qena, Egypt
Ayat A. Sayed
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, P.O.Box 71515, Assiut, Egypt
Ahmed El-Abd Ahmed
Department of Pediatrics, Qena Faculty of Medicine, South Valley University, P.O.Box No. 83523, Qena, Egypt
Tahia H. Saleem
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, P.O.Box 71515, Assiut, Egypt
Khalid I. Elsayh
Department of Pediatrics, Faculty of Medicine, Assiut University, P.O.Box 71515, Assiut, Egypt
Norhan B. B. Mohammed
Department of Medical Biochemistry and Molecular Biology, Qena Faculty of Medicine, South Valley University, P.O.Box No. 83523 Qena, Egypt
*Author to whom correspondence should be addressed.
Abstract
Aims: We evaluate the effect of enzyme replacement therapy "ERT" on the plasma levels of some biochemical markers of the disease burden, in pediatric patients with Gaucher's disease "GD", in the form of plasma chitotriosidase "ChT", total acid phosphatase activity, ferritin and globulin to evaluate the therapeutic monitoring efficacy of such biomarkers.
Methodology: A cross sectional case control study, carried out on 26 GD pediatric patients, divided into group A (13): On ERT and group B (13): Not receiving ERT, and in addition to 20 healthy age and sex matched controls. ELISA assays of plasma ChT and ferritin, colorimetric assays of plasma total acid phosphatase activity and globulin, were done for all groups, while, plasma proteins electrophoresis was done for GD patients only.
Results: Significant higher plasma levels of ChT, total acid phosphatase activity, ferritin and globulin among GD not receiving ERT versus both GD on ERT and Control group. Positive correlation between plasma ferritin and total acid phosphatase activity (r = 0.465 and P-value <0.05).
Conclusions: Plasma ChT, total acid phosphatase activity; ferritin and globulin are collectively helpful in evaluation and follow up the effect of ERT.
Keywords: Disease burden markers, Gaucher's disease, ERT