International Journal of Biochemistry Research & Review

Heavy Metals Effect on the Activity and Kinetics of Peroxidase Enzyme in Crude Extracts of Rosmarinus officinalis and Eruca sativa

Omar M. Atrooz *

Department of Biology, Mutah University, P.O.Box 7, Mutah, Jordan

Manar A. Al-Btoush

Department of Biology, Mutah University, P.O.Box 7, Mutah, Jordan

Ibrahim M. Al-Rawashdeh

Department of Biological Science, Al-Hussein Bin Talal University, P.O.Box 20, Maan, Jordan

*Author to whom correspondence should be addressed.

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Abstract

The catalytic activities and kinetic parameters of the enzyme peroxidase (POX), extracted from two plant species namely Rosmarinus officinalis and Eruca sativa, were investigated in the presence and absence of various heavy metals. The activation effects of heavy metals Co²⁺, Fe³⁺ and        Pb²⁺ have performed a noncompetitive inhibition on the enzyme activity in the crude extract of Eruca sativa. In contrast, Cd²⁺, Ni⁺ and Cu²⁺ have an uncompetitive inhibition for the two selected plants. Furthermore, Fe³⁺, Al³⁺, Pb²⁺ and Mo²⁺ were found to be acted as noncompetitive inhibitors on the enzyme activity in the crude extract of Rosmarinus officinalis. Presence of heavy metals altered the enzyme activity by acting as uncompetitive or noncompetitive inhibitors depending on the type of heavy metals.

Keywords: Relative activities, uncompetitive inhibition, noncompetitive inhibition

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