Premna integrifolia L. on Enzymatic Biomarkers in Atherosclerosis
S. Chitra *
Captain Srinivasamurthy Regional Ayurveda Drug Development Institute, CCRAS, M/o AYUSH, Govt. of India, Arignar Anna Hospital Campus, Arumbakkam, Chennai-600106, India
R. Arivukkodi
Captain Srinivasamurthy Regional Ayurveda Drug Development Institute, CCRAS, M/o AYUSH, Govt. of India, Arignar Anna Hospital Campus, Arumbakkam, Chennai-600106, India
S. Gaidhani
Central Council for Research in Ayurvedic Sciences, New Delhi-110058, India
R. Ilavarasan
Captain Srinivasamurthy Regional Ayurveda Drug Development Institute, CCRAS, M/o AYUSH, Govt. of India, Arignar Anna Hospital Campus, Arumbakkam, Chennai-600106, India
K. S. Dhiman Vd
Central Council for Research in Ayurvedic Sciences, New Delhi-110058, India
*Author to whom correspondence should be addressed.
Abstract
Premna integrifolia L. belongs to family Verbenaceae and it is believed to prevent cardiovascular disease as per Ayurveda. In order to evaluate the cardiac biomarkers and treatment efficacy of hydroalcoholic extract (HAE) of root bark in atherosclerosis. Sixty Wistar rats were divided into six groups and ten animals in each group. Rats fed with high fat diet and various concentrations of HAE treatment were studied and standard drug, atorvastatin was compared with HAE. This extract showed a significant action against atherosclerosis in a dose dependent manner which was studied in terms of lactate dehydrogenase activity, HMG-CoA/Mevalonate ratio, collagen content in aorta, levels of transaminases, calcium and other marker enzymes indirectly involved in the cardio protection. Alteration in collagen deposition in aorta of histopathological evaluation was noticed in treated animals. Anti-atherosclerotic activity of HAE of P. integrifolia was due to its modulatory activity on cholesterol metabolism and our results contribute towards validation of the traditional use of P. integrifolia in high fat diet induced atherosclerosis.
Keywords: HMG-CoA reductase, anti-atherosclerosis, collagen, aorta, agnimantha, lactate dehydrogenase