HPLC Profile of Bioactive Compounds Present in Olea europeae, Myrtus communis and Monotheca buxifolia and their Antibacterial Activities
Muhammad Zahoor *
Department of Chemistry, University of Malakand, Chakdara Dir (Lower) KPK, P.O. Box: 18000, Pakistan
Naveed Ahmad
Department of Chemistry, University of Malakand, Chakdara Dir (Lower) KPK, P.O. Box: 18000, Pakistan
Huma .
Department of Chemistry, University of Malakand, Chakdara Dir (Lower) KPK, P.O. Box: 18000, Pakistan
Farzana .
Department of Chemistry, University of Malakand, Chakdara Dir (Lower) KPK, P.O. Box: 18000, Pakistan
*Author to whom correspondence should be addressed.
Abstract
Aims: In this study the aqueous extract of Olea europeae, Myrtus communis and Monotheca buxifolia were screened for antibacterial activity, total phenolic content, polyphenol, chlorophyll a, chlorophyll b, β-carotene and lycopene.
Design of the Study: The study is composed of identification and quantification of bioactive compounds in selected plants through HPLC.
Place and Duration of the Study: Biochemistry Lab, Department of Chemistry University of Malakand Pakistan. The study duration was from September 2015 to August 2016.
Methodology: The antibacterial activities of the selected plants extracts were determined using agar well diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. The total polyphenol contents were determined by Follin Ciocalteu reagent method. The extract was subjected to HPLC analysis for antioxidants concentration.
Results: The zone of inhibition against Escherichia coli, Klebsiella pneumonia, Enterococcus faecalis and Bacillus cereus were 1.82, 1.52, 1.72 and 2.46 cm for Olea europeae extract, 1.51, 2.06, 1.98, 1.79 cm for Myrtus communis extract, 1.98, 2.28, 2.36, 1.62 cm for stem extract and 1.78, 2.05, 1.80, 1.77 cm for root extract of Monotheca buxifolia respectively. Morin, Quercetin and Chlorogenic acid were detected at retention times; 12.518, 10.022 and 6.305 minutes respectively in Olea europeae extract. Morein and Hydroxy benzoic acid were detected at 12.858 and 6.079 minutes retention time respectively in Myrtus communis extract. Chlorogenic acid, Quercetin, Morin, Pyrogallol and Phloroglucinol were detected in root extract of Monotheca buxifolia while in its stem, Chlorogenic acid, Quercetin, Morin, Rutin, Mandalic acid, Phloroglucinol and Hydroxy benzoic acid were detected.
Conclusion: A number of bioactive compounds were detected in the selected plants and on the basis of findings it was concluded that selected plants could be used medicinally.
Keywords: Oleaeuropeae, Myrtus communis, Monotheca buxifolia, antioxidants, pigments, inhibition