International Journal of Biochemistry Research & Review <p style="text-align: justify;"><strong>International Journal of Biochemistry Research &amp; Review (ISSN: 2231-086X)</strong> publishes original research papers, review articles and short communications on all areas of Biochemistry.&nbsp;This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed,&nbsp;open access INTERNATIONAL journal.</p> en-US (International Journal of Biochemistry Research & Review) (International Journal of Biochemistry Research & Review) Fri, 07 Feb 2020 09:47:05 +0000 OJS 60 Correlation of Leptin and Vaspin Serum Concentration in Newly Diagnosed Type 2 Diabetes Mellitus <p><strong>Objectives: </strong>The aim of the study was to explore the correlation of circulating leptin and vaspin levels with lipid profile, fasting blood sugar, HbA1c and anthropometric variable as inflammatory markers between diabetic patients and non-diabetic subjects.</p> <p><strong>Material and Methods: </strong>This study was conducted with 120 newly diagnosed type 2 diabetes mellitus (T2DM) patients with age-matched 120 non-diabetic subjects as controls.</p> <p><strong>Results: </strong>We found that there is significant increase in the parameters like serum Leptin, Vaspin, FBS, PPBS, HbA1c and lipid profile (TC, TG &amp; VLDL). No significant differences were found between BMI, LDL &amp; HDL parameters of T2DM patients compared to non-diabetic subjects. The results have been shown a significant positive correlation between Vaspin and Leptin in T2DM patients, (r = .755) and (<em>P˂0.01</em>) as compared to controls. The body mass index was positively correlated with Vaspin in T2DM patients, (r = .50) and (<em>P </em><em>&lt;0.01</em>) and with leptin in T2DM patients, (r = .265) and (<em>P </em><em>&lt;0.01)</em>. A positive correlation had also observed between vaspin and LDL in T2DM patients, (r = .189) and (<em>p </em><em>&lt;0.05</em>). We also found that significant increased level of leptin and vaspin in females compared to males in our study group.</p> <p><strong>Conclusions:</strong> Serum leptin and vaspin level is positively associated with BMI and LDL and negatively correlated with fasting blood sugar, post-prandial glucose, HbA1c, VLDL and age.</p> K. Priya, S. K. Bansal, D. K. Sharma, K. Y. Birendra ##submission.copyrightStatement## Fri, 07 Feb 2020 00:00:00 +0000 Effect of Leaf Extract of Senna alata on Biochemical Indices of Wistar Rats Infected with Trypanosoma brucei brucei <p>This study was aimed at determining the effect of leaves of <em>Senna alata </em>extract on biochemical indices of Wistar rats infected with <em>Trypanosoma brucei brucei</em>. Phytochemical screening revealed the absence of steroids in all extracts, absence of saponins in chloroform extracts and the presence of free anthraquinones only in chloroform extract. Post-infection treatment of animals stirred the emergence of parasitaemia by Day 3. Only animals receiving 200 mg/kg b.wt. of chloroform extract survived by day 16. A significant (P&lt;0.05) decrease in ALT for groups receiving methanol (400 mg/kg b.wt.), chloroform and aqueous extracts and significant (P&lt;0.05) increase in unconjugated bilirubin in the group receiving methanol extract (200 mg/kg b.wt.) compared to infected not treated rats. Significant (P&lt;0.05) decrease in potassium concentration in groups receiving methanol and chloroform, and a significant (P&lt;0.05) increase in sodium concentration in the group receiving 400 mg/kg b.wt. of aqueous extract compared to the infected not treated rats. These results thereby demonstrate the ameliorative potential of <em>Senna alata </em>leaves against <em>T. brucei brucei</em>.</p> M. A. Kugama, T. Tese, H. Sabo, T. Andrew, Y. A. Onaolapo, O. G. Allen, A. Agbo, D. W. Danjuma, A. M. Usman, L. M. Umar, P. J. Baji ##submission.copyrightStatement## Fri, 07 Feb 2020 00:00:00 +0000 Effects of Repeated Administration of Extracts from Arachis hypogaea Hulls on Blood Parameters and Histological Organization of Heart, Liver and Kidneys of Rats <p><strong>Aims: </strong><em>Arachis hypogaea</em> (peanuts) is widely used in food worldwide. Therapeutic use of various parts of this plant has been mentioned in many traditional medicinal systems. The aim of the present study was to evaluate subacute toxicity of methanolic and aqueous extracts of peanuts hulls.</p> <p><strong>Place and Duration: </strong>Laboratory of Biochemical Pharmacodynamics, Faculty of Biosciences, University Félix Houphouët-Boigny, from March to June 2018.</p> <p><strong>Methodology: </strong>Serial extraction was done using methanol and water as solvents. The study was evaluated by orally daily doses of extracts 300, 600 and 1200 mg/kg. The treatment for 28 days concerned seven groups of animals, the control group and six treated groups. Each group included six animals, 3 males and 3 females. Animals of control group didn’t have any treatment. Animals were observed for general behavioural and signs of abnormalities during the experiment. After all treatments, blood was collected for haematological and biochemical analysis. Liver, kidney, and heart were removed, weighed for histological study.</p> <p><strong>Results:</strong> The results showed that, there were not any significant (p˃0.05) changes in both the absolute and relative organ weights between the control and the test groups. Biochemical parameters were statistically equal in all groups. In addition, both extracts did not induce any significant effect on RBC and indices relating to it (HGB, PCV, MCV, MCH and MCHC) throughout the experimental period. But, there was a decrease (16.33±1.68) on WBC with methanolic extract compared to control (13.79±2.73). Histological examination of the liver, kidneys, and the heart showed normal organisation and structure of heart, kidneys and liver.</p> <p><strong>Conclusion:</strong> It appears that the methanolic and aqueous extracts of hull of <em>Arachis hypogaea</em> did not produce any toxicity in oral subacute toxicity study. However, further studies are needed to confirm long term toxicities.</p> Djyh Bernard Nazaire, Tra Bi Irié Otis, Oungbé Monkoué Désiré, Gnahoué Goueh Laurent, Kra Adou Koffi Mathieu, D’almeida Marie-Anne Kayi, Djaman Allico Joseph ##submission.copyrightStatement## Wed, 19 Feb 2020 00:00:00 +0000