International Journal of Biochemistry Research & Review http://journalijbcrr.com/index.php/IJBCRR <p style="text-align: justify;"><strong>International Journal of Biochemistry Research &amp; Review (ISSN: 2231-086X)</strong> publishes original research papers, review articles and short communications on all areas of Biochemistry.&nbsp;This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed,&nbsp;open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US International Journal of Biochemistry Research & Review 2231-086X Effect of Oral Administration of Diclofenac Sodium on Coagulation Factors and Some Hematological Parameters in Wister Rats http://journalijbcrr.com/index.php/IJBCRR/article/view/30154 <p>Diclofenac sodium is a nonsteroidal anti-inflammatory drug often obtainable as a prescription drug or over the counter. It is very effective in the control of inflammation and pain due to arthritis or pains arising following many disease conditions because of its antipyretic, anti inflammatory and analgesic potentials. Despite the beneficial effects of diclofenac sodium, it has been implicated in some adverse effects. In this study, we examined the effect of acute and chronic administration of diclofenac sodium on some hematological (PCV, WBC differentials) and coagulation&nbsp; (prothrombin&nbsp; time,&nbsp; activated partial prothrombin time and platelets count) parameters of albino Wister rats using the standard methods.&nbsp; Twenty four Albino Wister rats were divided into three groups of 8 rats and grouped as control, acute study and chronic study. The rats were administered 0.2 mg of diclofenac sodium for 24 hours for acute and 3 weeks for chronic studies respectively. The rats were sacrificed and blood collected for analysis of PCV, WBC differentials, prothrombin time, activated partial prothrombin time and platelets count using the&nbsp; standard&nbsp; methods.&nbsp; Results&nbsp; show&nbsp; that&nbsp; acute&nbsp; administration&nbsp; of&nbsp; diclofenac sodium&nbsp; at&nbsp; 0.2 mg&nbsp; has&nbsp; no&nbsp; effect&nbsp; on&nbsp; hematological&nbsp; and&nbsp; coagulation&nbsp; parameters,&nbsp; but chronic&nbsp; administration&nbsp; could&nbsp; instigate&nbsp; significant&nbsp; reduction&nbsp; in&nbsp; PCV,&nbsp; platelets&nbsp; count, neutrophils and monocytes (p&lt;0.001), while there is a significant increase in PT, INR, lymphocytes&nbsp; (p&lt;0.001).&nbsp; Considering these alterations, it is advisable that&nbsp; this drug should be made a strictly prescription drug in order to prevent indiscriminate use of this medication and to prevent attendant anemia and coagulopathy that may follow chronic use.</p> Abraham Zorte Jonathan Esima Waribo Helen Onwuli, Donatus O. ##submission.copyrightStatement## 2019-12-28 2019-12-28 1 7 10.9734/ijbcrr/2019/v28i430154 Influence of Fractional Composition of Dietary Supplements "Majmui Rahmoniy" on Mycobacterium smegmatis http://journalijbcrr.com/index.php/IJBCRR/article/view/30155 <p>Despite the availability of anti-TB drugs, the proportion of cases of multidrug-resistant tuberculosis (MDR) is increasing. One of the important tasks to identify effective drugs in nature is the study of numerous experiments of traditional medicine.&nbsp; This work is devoted to the study of chemical, biochemical, medical evaluation of the new therapeutic food additive "Majmui Rahmoniy", and its effective effect on <em>Mycobacterium smegmatis</em> and&nbsp; MS Rv2349c gene which responsible for the phospholipase activity.</p> Irodakhon Mamatova Mukhamatdin Mamarakhmonov Ibragim Askarov ##submission.copyrightStatement## 2020-01-04 2020-01-04 1 6 10.9734/ijbcrr/2019/v28i430155 Effects of Storage on Nutritional, Mineral Composition and Mycoflora of Stored Sundried Citrullus lanatus Thunberg (Melon) Seeds http://journalijbcrr.com/index.php/IJBCRR/article/view/30149 <p>The effect of storage on the nutritive values, proximate composition and mycoflora of <em>Citrullus lanatus</em> was investigated during a 24 weeks of storage. The mycoflora were isolated using direct plating and washing methods. Eight fungi were isolated namely <em>Fusarium </em>sp<em>.</em>,<em> Rhizopus </em>sp<em>., Mucor </em>sp<em>., Aspergillus niger, A. fumigatus, A. flavus, Penicillium chrysogenum</em> and <em>Penicillium</em> sp. The fungal count was found to increase as the storage time increased. The proximate analysis showed a decrease and an increase on some parameters investigated. The mineral composition was found to decrease as the storage time increased. The following nutrients (g/100 g) were found to have reduced: ash content 4.29 - 3.19, fibre content 2.06 - 1.51, fat content 57.02 - 50.90, while the following increased: Moisture content 7.21-12.22, crude protein 29.24-30.90, carbohydrate content 0.20-2.01. The results for minerals analysis (mg/100 g) showed a decrease in all parameters investigated: sodium 2.01-1.49, potassium 65.52-56.21, calcium 13.10-10.19, magnesium 46.34-37.61, Iron 8.12-3.31, zinc 4.76-1.24, copper 2.84-2.11 and phosphorus 3.46-1.60 while lead and cadmium were not detected. However, storage was found to cause deterioration and reduction in the nutritive value and mineral content of<em> C. lanatus</em> seeds. It is therefore recommended that these seeds must be stored under controlled conditions that will prevent the growth of fungi and reduction of nutrients.</p> Emmanuel Dayo Fagbohun Ayodeji Segun Ogundahunsi ##submission.copyrightStatement## 2019-12-13 2019-12-13 1 7 10.9734/ijbcrr/2019/v28i430149 Evaluation of Lifestyle and Some Biochemical Parameters Involved in Bone Health in Pre and Postmenopausal Cameroonian Women http://journalijbcrr.com/index.php/IJBCRR/article/view/30150 <p><strong>Aims: </strong>A healthy skeletal system with strong bones is essential to overall health. This study aimed to assess lifestyle and bone’s biomarkers of women.</p> <p><strong>Study Design:</strong> This was a cross-sectional study.</p> <p><strong>Place and Duration of Study:</strong> The enrollment took place at the Yaounde Military Hospital from November 2016 to July 2017.</p> <p><strong>Methodology:</strong> A set of 105 postmenopausal and 127 premenopausal participants were enrolled from November 2016 to July 2017 in Yaounde. Their physical activities level was evaluated using a questionnaire. Sunlight exposure between 10:00AM and 4:00PM was assessed. Colorimetric methods were used to evaluate calcium, albumin, and alkaline phosphatase activity in plasma. These parameters were then compared within year since menopause (YSM) and osteoporosis risk assessment instrument (ORAI) sub-groups.</p> <p><strong>Results:</strong> 13.4% participants were moderately active. Only 22.8% participants had at least 30 minutes sun exposure daily. A multivariable analysis of demographic, lifestyle, biochemical characteristic revealed age, BMI, albumin and calcium were the main factors that influenced bone health among our participants. A significant difference of calcium concentration was found between the two groups whereas albumin was significantly higher in premenopausal than postmenopausal. Albumin was the only variable with a significant difference in the YSM sub-groups while only calcium was non-significantly higher among the ORAI sub-groups.</p> <p><strong>Conclusion:</strong> Elevated plasma level of calcium, albumin and alkaline phosphatase could be indicators of high bone turnover.</p> Gerard Nkeunen Tsafack Takadong Julie Judith Simo Gustave Betrand Ayuk Tambe Ayangma Celestin Roger Tume Christopher Bonglavnyuy ##submission.copyrightStatement## 2019-12-14 2019-12-14 1 11 10.9734/ijbcrr/2019/v28i430150 Nephrotoxicological Effect of Water Soluble Fraction of Bonny Light Crude Oil in Wistar Albino Rats http://journalijbcrr.com/index.php/IJBCRR/article/view/30151 <p>This study evaluated the Nephrotoxic effect of water soluble fraction (WSF) of Bonny Light Crude Oil (BLCO). After preparation of the WSF and a range finding test, the Wistar albino rats were administered three concentrations (25%, 50% and 100%) of WSF of BLCO for 30 and 60days. Data from the study showed that Urea concentration increased significantly (p≤0.05) with increasing dose of BLCO ranging from 14.71 mg/dl in the control to 35.28 mg/dl in the 100% group after 30days and 14.28 mg/dl in the control to 41.08mg/dl in the 100% group after 60days, Creatinine concentration increased significantly (p≤0.05) from 0.22 mg/dl in the control to 0.82mg/dl in the 100% group after 60 days administration while electrolyte (Na, K, Cl) concentration increased significantly (p≤0.05) with increasing dose of BLCO after 60days administration. Histopathological examination of the kidney was characterized by partial partitioning of the glomerular tufts, obliteration of the Bowman’s capsule and distortion of the renal tubules. The findings in this research suggest that WSF of BLCO induced nephrotoxicity.</p> Abraham, Chiedozie Nicholas J. Udom, Godwin C. Patrick-Iwuanyanwu, Kingsley ##submission.copyrightStatement## 2019-12-14 2019-12-14 1 9 10.9734/ijbcrr/2019/v28i430151 Effect of Chelation Therapy and Blood Transfusion on Trace Elements in Thalassemia Patients http://journalijbcrr.com/index.php/IJBCRR/article/view/30152 <p><strong>Background: </strong>β-Thalassemia major is considered to be one of the most common inherited hemolytic anemia. Enhanced years of survival of thalassemia have led to unmasking related complications related to alterations in certain trace elements like magnesium, calcium, phosphorus, copper, zinc etc.</p> <p><strong>Objective:</strong> Present study was conducted to evaluate the effect of iron chelation therapy and blood transfusion on certain trace elements (Magnesium, Calcium, Phosphorus, Copper, Zinc) in β-thalassemic patients on chelation therapy more than one year.</p> <p><strong>Materials and Methods:</strong> In the present cross sectional study, 100 β-thalassemic patients receiving chelation therapy for atleast 1year were recruited from Civil Hospital Ahmedabad, Gujarat during February, 2017 to December, 2018 and equal&nbsp; number (n=100) of healthy subjects were taken as a control group in the age range of 8 to 15 years of both sexes (male &amp; female). The levels of serum magnesium, calcium, phosphorus, zinc, and copper in serum were analyzed and results were correlated with normal healthy subjects.</p> <p><strong>Results:</strong> A significant increase in serum copper (P≤ 0.01) and phosphorus (P≤ 0.001) were observed levels while a significant (P≤ 0.05) fall in magnesium, calcium and zinc levels recorded in β-thalassemic patients in comparison to healthy control subjects.</p> <p><strong>Conclusion:</strong> Aforementioned observations suggested that fluctuations in the trace elements levels in β- thalassemic children receiving blood transfusion and iron chelation therapy could leads to different complications like hemolyzed red cells, infections &amp; hemochromatosis renal damage, hypoparathyroidism etc. if remains untreated. Hence routine assessment of these elements is recommended for better management.</p> Vijaysinh R. Parmar Kuldip Singh Asha Khubchandani ##submission.copyrightStatement## 2019-12-18 2019-12-18 1 5 10.9734/ijbcrr/2019/v28i430152 Inhibitors of Cellulase Activities According to the Trophic Group of Termites (Insecta: Isoptera) from Daloa (Côte d'Ivoire) http://journalijbcrr.com/index.php/IJBCRR/article/view/30153 <p>The presence of termites in the cocoa plantations and quarries of Côte d'Ivoire poses a threat to the producers of this sector. Producer yields are insufficient to cover the strong market demand. This situation leads to food insecurity for the population. Knowledge of the specific inhibitory molecules of digestive enzymes of termites is necessary to enhance the effectiveness of insecticides to optimize crop production. The present study was aimed to characterize termite cellulases according to the trophic group. Specifically, the influence of chemical agents on the cellulase activities of four humivorous (<em>Cubitermes fungifaber</em>) and xylophagous termites (<em>Nasutitermes latifrons</em>, <em>Microcerotermes fuscotibialis</em> and <em>Amitermes guineensis</em>) collected in Daloa during the October period was investigated. Thus, the cellulase activities were measured by the spectrophotometric method in the absence and in the presence of the concentrations of 1 and 5 mM of various chemical agents. The chemical agents used behaved differently on cellulase activities. Thus, Cu<sup>2+</sup>, Pb<sup>2+</sup> and EDTA inhibited the cellulase activity of <em>M. fuscotibialis</em> more than 90% at concentrations of 1 and 5 mM, respectively, indicating the presence of a metalloprotein. On the other hand, that of the other two xylophagous species was slightly inhibited. In addition, the cellulase activity of C. Fungifaber was inhibited at the two respective concentrations by Cu<sup>2+</sup> at about 70%. In conclusion, Cu<sup>2+</sup>, pb<sup>2+</sup> and EDTA can be used in the formulation of some specific insecticides against humivorous and xylophagous termites.</p> Blei Sika Hortense Tra Bi Crolaud Sylvain Eba Krou Philippe Beugre Avit Grah Maxwell Faulet Meuwiah Betty Niamke Lamine Sebastien Kouame Lucien Patrice ##submission.copyrightStatement## 2019-12-24 2019-12-24 1 7 10.9734/ijbcrr/2019/v28i430153 Enzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approaches http://journalijbcrr.com/index.php/IJBCRR/article/view/30156 <p>Concerned with the construction and design of novel biocatalysts, the enzyme engineering served to overcome the limitations of native enzymes, in order to create biocatalysts with tailored functions, to facilitate industrial applications. The enzymes, being recognized by screening and discovery workflows and further tailored by engineering platforms, are of immense potential as improved biocatalysts. Functional metagenomics is a powerful tool to identify novel enzymes followed by the construction of metagenome-based enzyme libraries. And the subsequent screening of these enzyme libraries is in turn facilitated by ultra-high-throughput-based, for example FACS or microfluidics, enzyme engineering technologies. Relies on the compartmentalization of reaction components, in order to detect and measure assay signal within the reaction compartments, the enzyme engineering platforms are designed which include cell-as-compartment platforms, droplet-based platforms and micro-chamber-based platforms. The metagenomics approach and high-throughput screening by these three prime enzyme engineer platforms are the focus of this review.</p> Sikander Ali Syed Shahid Hussain ##submission.copyrightStatement## 2020-01-20 2020-01-20 1 12 10.9734/ijbcrr/2019/v28i430156