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Aims: Spigelia anthelmia is a medicinal plant used in the treatment of diverse diseases and contain plant-based natural bio-active constituents.
Study Design: Phytochemical and mineral analysis of Spigelia anthelmia leaves using standard analytical procedures.
Place and Duration of Study: Forestry Research Institute of Nigeria, between May 2019 and July 2019.
Methodology: Phytochemical and phytomineral status of Spigelia anthelmia leaves were investigated using standard analytical procedures. Phytochemicals screening/analysis examined include; alkaloids, flavonoids, saponin, tannin, phenolics, cardiac glycosides, phlobatannin and terpenoids. The atomic absorption spectrophotometer (AAS) was used to determine the minerals which are calcium (Ca), phosphorus (P), magnesium (Mg), iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu), while flame photometer was used in determining potassium (K) and sodium (Na). Data were presented using descriptive statistics (mean and standard deviation).
Results: Result indicates the presence of phytochemicals in the leaves of S. anthelmia, the quantitative analysis shows: alkaloid (2.34mg/100g), flavonoid (6.13mg/100g), saponin (18.12mg/100g), tannin (9.61mg/100g), phenol (4.61mg/100g), cardiac glycosides (1.89mg/100g), terpenoids (0.98mg/100g) and phlobatannin (0.32mg/100g). The macro elements reveals Na (0.022%), K (0.23%), Ca (0.48%), Mg (0.15%), P (0.035%), while the micro element shows that Fe (0.19%), Mn (0.003%), Cu (0.001%), Zn (0.004%).
Conclusion: The result suggests that S. anthelmia has a strong pharmacological prowess and could be used in the prevention and treatment of various health-related diseases.
Ghani A. Introduction to pharmacognosy. Ahmadu Bello University Press, Nigeria. 1990;1(2):199-205.
Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis, 13th Ed. Chapman and Hall, Ltd. London. 1973;5-15.
Arts CW, Hollman PC. Polyphenols and disease risk in epidemiologic studies. Am J ClinNutr. 2005;81:317-325.
Martinko MMT, John M, Parker J, Brock biology of microorganisms (8th edn.). Prentice Hall, USA; 1996.
Olorunfemi CJ, Joseph OA, Ambrose OA, Rowland ISA. Anthelmintic efficacy of extracts of spigelia anthelmintic of extracts of Spigelia anthelmia Linn on experimental Nippostrongylus braziliensis in rats, J. Vet. Sci. 2006;7:229-232.
Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouring agents. Global J. Appl. Sci. 2001;7:455-459.
Uzama Danlami, Osuagwu Eberechi Cecilia, Osuagwu Magnus Ifeanyi. Evaluation of the phytochemicals and antimicrobial activities of the ethanolic, Hexane and ethyl acetate extracts of Spigelia anthelmia leaves. International Journal of Pharmacy and Chemistry. 2017; 3(3):29-32.
Mbaebie BO, Edeoga HO, Afolayan AJ. Phytochemical analysis and antioxidants activities of aqueous of stem bark extract of Schotia latifolia Jacq. Asian Pacific Journal of Tropical Biomedicine. 2012;2: 118-124.
Association of Official Analytical Chemists (AOAC). Official methods of analysis. Food composition, additives and natural contaminants. Aldric, RC 15th edn. Association of Official Analytical Chemists Inc. USA; 2005.
Costa MA, Zia ZQ, Davin LB, Lewis NG. Chapter four: Toward engineering the Metabolic pathways of cancer-preventing lignans in cereal grains and other crops. In Recent Advances in Phytochemistry, Phytochemicals in Human Health Protection, Nutrition and Plant Defense, ed. JT Romeo, New York. 1999;67-87.
Zulak KG, Liscombe DK, Ashihara H, et al. Alkaloids, in Crozier A, Clifford MN, Ashihara H. (eds), Plant secondary metabolites: Occurrence, structure and role in the human diet, Blackwell Publishing Limited, Oxford, UK. 2006: 102–136.
Narasinga Rao, Bioactive phytochemicals in Indian foods and their potential in health promotion and disease prevention. Asia Pacific Journal of Clinical Nutrition. 2003; 12:9-22.
Fagbohun ED, Lawal OU, Ore ME, The proximate, mineral and phytochemical analysis of the leaves of Ocimum gratissimum, Melanthera scandens, Leea guineensis and their medicinal value. International Journal of Applied Biology and Pharmaceutical Technology. 2012;3: 15-22.
Awotedu OL, Ogunbamowo PO, Emmanuel IB, Lawal IO, Phytominerals and phytochemical studies of Azadirachta indica, Leea guineensis and Parkia biglobosa leaves. International Annals of Science. 2019;6:28-34.
Ghosal S, Krishima Prasad BN, Lakshmi V, Antiamoebic activity of piper longum fruits against Entamoeba histolytica in vitro and in vivo. Journal of ethno-pharmacology. 1996;50:167-170.
Awoyinka AO, Balogun IO, Ogunnowo AA, Phytochemical screening and in vitro bioactivity of Cnidoscolus aconitiifolus (Euphorbiaceae). Journal of Medicinal Plants Research. 2007;1:63-65.
Asquith TN, Butter LG, Interaction of condensed tannins with selected proteins. Phytochemistry. 1986;25:1591-1593.
Jimoh F.O.* and Oladiji A.T. Preliminary Studies on Piliostigma thonningii seeds: Proximate analysis, mineral composition and phytochemical screening. African Journal of Biotechnology. 2005;4:1439-1442.
Garg Praveen, Garg Rajesh. Qualitative and quantitative analysis of leaves and stem of Tinospora cordifolia in different solvent extract. Journal of Drug Delivery & Therapeutics. 2018;8:259-264.
Godswill N. Anyasor K, Olusola Ogunwenmo, Olatunji A. Oyelana, Blessing E. Akpofunure. Phytochemical constituents and antioxidant activities of aqueous and methanol stem extracts of Costus afer Ker Gawl. (Costaceae). African Journal of Biotechnology. 2010;9: 4880-4884.
Rajput SS, Soni KK, Saxena RC, Pharmacology and phytochemistry of saponin isolated from Aloe vera for wound healing activity. Asian J Chem. 2009;21: 1029-1032.
Ndukwe OK, Awomukwu D, Ukpabi CF, Comparative evaluation of phytochemical and mineral constituents of the leaves of some medicinal plants in Abia State Nigeria. International Journal of Academic Research in Progressive Education and Development. 2013;2:244-252.
Harry-Asobara JL, Samson EO. Comparative study of the phytochemical properties of Jatropha curcas and Azadirachta indica plant extracts. Journal of Poisonous and Medicinal Plants Research. 2014;2:020-024.
Okeke IC, Ezeabara CA, Phytochemical screening and in vitro anti microbial activity of various parts of Cleome ciliate Schum. & Thonn. Bioscience Horizons. 2018;11:1-7.
Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, Asnad. Phyto chemical analysis of medicinal plants occurring in local area of Mardan. Biochem Analyt Biochem. 2013;2:144.
Mahato SB, Sen S, Advances in triterpenoid research, Phytochemistry. 1997;44:1185-1236.
Fernandez-Panchon MS, Villano D, Troncoso AM, Garcia-Parrilla MC. Antioxidant activity of phenolic compounds: From in vitro results to in vivo evidence. Crit Rev Food SciNutr. 2008;48:649-71.
Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol. 2018;8:97- 101.
Jones GA, McAllister TA, Muir AD, Cheng KJ, Effects of saponin (Onobrychis viciifolia Scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Appl. Environ. Microbiology. 1994;60:1374-1378.
Ekam VS, Ebong PE, Serum protein and enzymes levels in rats following administration of antioxidant vitamins during caffeinated and non-caffeinated paracetamol induced hepatotoxicity. Nigeria. Journal of Physiology Science. 2007;22:65-68.
Oluwasina OO, Olagboye SA, Olaiya A, Hassan FG, Comparative study on phytochemical quantification and antimicrobial activity of Raufolvia vomitoria leaves, seeds and root extracts. FUTA Journal of Research in Sciences. 2017;13: 10-16.
Dewick PM, Medicinal Natural Products A biosynthetic approach, 2nd Ed. ed. John Wiley and Sons Ltd; 2002.
Benbott OO, Yahyia A, Belaidi A, Assessment of the antibacterial activity crude alkaloids extracted from seeds and roots of the plant Peganu mharmala. Journal of Natural Products and Plant Resources. 2012;2:568-573.
Russo P, Frutaci A, Del Bufalo A, Fini M, Cesario A. Multi target drugs of plants origin acting on “Alzheimer’s disease” Curr Med Chem. 2013;20:168-193.
Cushnie TP, Cushnie B, Lamb AJ, Alkaloids: An overview of their bacterial, antibiotic-enhancing and antivirulence activities. Int. J. antimicro Agent. 2014;44: 377-386.
Jisika M, Ohigashi H, Nogaka H, Tada T, Hirota M. Bitter steroid glycosides, Vernon sides A1, A2 and A3 and related B1 from the possible medicinal plant Vernonia amygdalina used by wild Chimpanzees. Tetrahedron. 1992;48:625- 630.
Jamuna Senguttuvan, Subramaniam Paulsamy, Krishnamoorthy Karthika, Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed. 2014;4:359-S367.
Ekwueme FN, Nwodo OFC, Joshua PE, Nkwocha C, Eluka PE, Qualitative and quantitative phytochemical screening of the aqueous leaf extract of Senna mimosoides: Its effect in in vivo leukocyte mobilization induced by inflammatory stimulus. Int. J. Curr. Microbiol. App. Sci. 2015;4:1176-1188.
Ajuru MG, Williams LF, Ajuru G, Qualitative and quantitative phytochemical screening of some plants used in ethnomedicine in the Niger Delta Region of Nigeria. Journal of Food and Nutrition Sciences. 2017;5:198-205.
Ajuru MG, Nmom FW, Oghenerukevwe OD. Qualitative and quantitative phytochemical screening of some species of Lamiaceae in Rivers State, Nigeria. Research Journal of Food and Nutrition. 2018;2:28-37.
Norton BW. The significance of tannins in tropical animal production. Tannins in Livestock and Human Nutrition. ACIAR Proceedings No. 2000; 92:14-2.
Olaniyi MB, Lawal IO. Olaniyi AA. Proximate, phyto chemical screening and mineral analysis of Crescentia cujete L. leaves. Journal of Medicinal Plants for Economic Development. 2018;2: a28.
Awotedu OL, Ogunbamowo PO. Nutritional, anti-nutritional and phytochemical profile of the leaves and fruits of Synsepalum dulcificum (Schumach. & Thonn.) Daniell. American Journal of Biological Chemistry. 2019;7(3): 53-59
Hassan LG, Abdulrahaman FN, Zuru AA, Nutritional and phytochemical investigation of Diospyros mespiliformis (L.)’, Nigerian Journal of Basic Applied Science. 2004;13: 1–8.
Ahmed D, Chandhary MA. Medicinal and nutritional aspects of various trace metals determined in Ajugabracteosa. Journal of Applied Sciences Research. 2009;5:864–869.
Chavan YR, Thite SV, Aparadh VT, Kore BA, Quantification of minerals in some members of family Asteraceae. World journal of Pharmaceutical Research. 2014;3:613-617.
Vormann J. Magnesium: Nutrition and metabolism. Molecular Aspects of Medicine. 2003;24:27–37.
F. N. D. Food and Nutrition Board, Institute of Medicines. National Academy of Sciences. Dietary reference intake for energy, carbohydrate, fibre, fat, fatty acids, cholesterol, protein and amino acid (Micronutrients); 2002.
Ujowundu CO, Kalu FN, Okafor OE, Agha NC, Alisi CS, Nwaoguikpe RN, Evaluation of the chemical composition of Dacryodes edulis (G. Don) seeds. Int. J. Biol. Chem. Sci. 2010;4: 1225-1233.