Effects of Storage on Nutritional, Mineral Composition and Mycoflora of Stored Sundried Citrullus lanatus Thunberg (Melon) Seeds

Main Article Content

Emmanuel Dayo Fagbohun
Ayodeji Segun Ogundahunsi

Abstract

The effect of storage on the nutritive values, proximate composition and mycoflora of Citrullus lanatus was investigated during a 24 weeks of storage. The mycoflora were isolated using direct plating and washing methods. Eight fungi were isolated namely Fusarium sp., Rhizopus sp., Mucor sp., Aspergillus niger, A. fumigatus, A. flavus, Penicillium chrysogenum and Penicillium sp. The fungal count was found to increase as the storage time increased. The proximate analysis showed a decrease and an increase on some parameters investigated. The mineral composition was found to decrease as the storage time increased. The following nutrients (g/100 g) were found to have reduced: ash content 4.29 - 3.19, fibre content 2.06 - 1.51, fat content 57.02 - 50.90, while the following increased: Moisture content 7.21-12.22, crude protein 29.24-30.90, carbohydrate content 0.20-2.01. The results for minerals analysis (mg/100 g) showed a decrease in all parameters investigated: sodium 2.01-1.49, potassium 65.52-56.21, calcium 13.10-10.19, magnesium 46.34-37.61, Iron 8.12-3.31, zinc 4.76-1.24, copper 2.84-2.11 and phosphorus 3.46-1.60 while lead and cadmium were not detected. However, storage was found to cause deterioration and reduction in the nutritive value and mineral content of C. lanatus seeds. It is therefore recommended that these seeds must be stored under controlled conditions that will prevent the growth of fungi and reduction of nutrients.

Keywords:
Mycoflora, shelled melon, proximate, minerals, Citrullus lanatus

Article Details

How to Cite
Fagbohun, E. D., & Ogundahunsi, A. S. (2019). Effects of Storage on Nutritional, Mineral Composition and Mycoflora of Stored Sundried Citrullus lanatus Thunberg (Melon) Seeds. International Journal of Biochemistry Research & Review, 28(4), 1-7. https://doi.org/10.9734/ijbcrr/2019/v28i430149
Section
Original Research Article

References

Huxley A. The new RHS dictionary of gardening. Macmillan Press. Long Island, New York. 1992;5-8.

Pearson, J. Gardening column: Watermelons; 2002.
Available:http://www.plantanswers.com/garden_column/june5.html

Duke JA, Ayensu ES. Medicinal Plants of China. Reference Publications, Inc; 1985.

Wolford R, Banks O, Drusilla A. Watch your garden grow. Water melon; 2005.
Avaiable:www.extension.illinois.edu/veggies/watermelon.cfm

National Research Council (NRC). Lost Crops of Africa: Volume II: Vegetables. Washington, DC: The National Academies Press. 2006;155-171.
Avaliable:https://doi.org/10.17226/11763

Abaka-Gyenin AK, Norman J C. The effect of storage on fruit quality of watermelons (Citrullus vulgaris Schad) ISHS. Acta Horticulturae 53: IV Africa symposium on Horticultural crops; 2000.

Fogbound ED, Abegunde OK, David O M. Nutritional and mycoflora changes during storage of plantain chips and the health implications. J. Agric. Biotechnol. and Sustain. Dev. 2010;2(4):61- 65.

Amusa NA, Kehinde IA, Ashaye OA. Biodeterioration of the Africa Star Apple (Artocarpus communis) in storage and it's effects on the nutrient composition. Afr. J. of Biotechnol. 2002;1(2):57- 60.

Burell NJ. Chilling in storage of cereal, grain and their products. C. M. Christensen (Editor) American Association of cereal chemists. Inc., St. Paul MN; 1974.

Aderiye BI. Contributory roles of microbes to human development. 10th Inaugural Lecture, University of Ado Ekiti, Nigeria. 2004;11- 36.

Kuku FO. Deterioration of melon seeds during storage at various relative humidities. Republic of Nigerian Stored Products Research Institute Technical Report. 1979;7:65-67.

Ekundayo CA, Idzi E. Mycoflora and nutritional value of shelled melon seeds (Citrullus vulgaris schrad) in Nigeria. J. Plant Foods for Human Nutrition. 2005;40:31-40.

Muller JD. In: Fungi and mycotoxin in stored products ACIAR proceedings No: 36 (BR Champ, Ettighealy, AD Hocking and JJ Pitt editors), Australian center for Int. Res. 1991;126-135.

Richard JL, Wallace HA. Mycotoxins, National center for Agricultural Utilization Research, USDA/ARS; 2001.

Lawal OU, Fagbohun ED. Studies on Bio-Deterioration, Aflatoxin Contamination and Nutritive Values of Processed Cashew (Anacardium occidentale L) Nuts during Storage. Nature and science. 2014;11(9): 127-133.

Fagbohun ED, Oluwaniyi TT. Mycoflora, Proximate Composition and Nutritional changes during the storage of Oryza sativa. Food Science and Quality Management. 2015;40:108-116.

Amusa NA. Fungi associated with yam tubers in storage and the effect on the chip nutrient composition. Moor J. Agric. Res. 2001;2:35-39.

Arotupin DJ, Akinyosoye FA. Microflora of sawdust. Niger. J. Microbiol. 2001;15(1): 97-100.

Fagbohun ED, Egbebi AO, Anibijuwon II, Lawal OU. Fungi associated with spoilage of dried cocoa beans during storage in Ekiti state of Nigeria. J. Microbiol. Biotech and Food Sc. 2011;6(3).

Swanson KMJ, Busta FF, Peterson EH. Colony count methods. In: Vanderzant, C., Splittoesser, D.S. Compedium of Methods for the Microbiological Examination of Foods, 3rd ed. American Public Health Association, New York. 1992;75-95.

Barnett HL, Hunter BB. “Illustrated Genera Of Imperfect Fungi” MacMikkan Publishing Company, New York; 1987.

Domsch KH, Gams W, Anderson TW. Compedium of soil fungi. Academic Press, London. 1993;860.

Moubasher AH. Soil fungi in Qatar and other Arab countries. Scientific and Applied Research Center, Qatar University, Doha. 1993;566.

Pitt J, Hocking AD. Fungi and Food Spoilage. Springer. Verlag, USA. 2009;21-57.

Crowley N, Bradley JM, Darrell JH. Practical bacteriology. Butterworth and Co., Ltd. London, 1969;164-168.

AOAC. Official Method of Analysis. 14th Ed. Association of Official Analytical Chemist, Washington. DC; 2005.

Kothari CR. Research methodology, methods and techniques. New Age Inter-national Publishers, India. 2004; 256-264.

Nair LN. Topics in Mycology and Pathology. New Central Book Agency, Kolkata. 2007;363-373.

Bankole SA. Moisture content, mould invasion and seed germinability of stored melon. Mycopathologia. 1993;122(2):123-126.

Aboaba OO, Amasike J. Storage of melons seeds, Nigeria Journal of Botany. 1991;4: 213-219.

Amadi JE, Adebola MO. Effect of moisture content and storage conditions on the stability of garri. Afri. J. Biotechnol. 2008; 7(24):4591- 4594.

Ogundana SK, Naqvi SHI, Ekundayo JA. Fungi Associated with soft rot of yams (Dioscorea spp) in storage in Nigeria. Trans. Br. Mycol. Soc. 1970;54(3):445-451.

Burnett JH. Fundamentals of Mycology. Edward Arnold (Pub) Ltd. London. 1995; 145-187.

Orsi RB, Correa B, Possi CR, Schammass EA, Nogueira JR, Dias SMC, Malozzi MAB. Mycoflora and occurrence of fumonisins in freshly harvested and stored hybrid maize. J. Stored Products Research. 2000;36:75-87.

Onifade AK, Jeff-Agboola YA. Effect of fungal infection on proximate nutrient composition of coconut (Cocos nucifera Linn) fruit. J. Food Agric Environ. 2003; 10(2):30-33.

Rice RG, Graham DM, Lowe MT. Recent ozone applications in food processing and sanitation. Food Safety Magazine. 2002; 8(5):10 -17.

FAO. Bacillus cereus and other Bacillus sp. U. S. Food and Drug Administration Center for Food Safety and Applied Nutrition, Bed Bug Book. Food borne pathogenic microorganisms and natural toxin handbook; 2002.
Available:http//www.cfsan.fda.gov/mow/chap12.html

Bankole SA, Osho A, Joda AO, Enikuomehin OA. Effect of drying method on the quality and storability of 'egusi' melon seeds (Colocynthis citrullus L.). Nigerian Journal of Botany. 2005;22(1): 73-80.