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Aims: The aim of the study was to evaluate the status and diagnostic utility of PON1. (Paraoxonase-1) Arylesterase and nitric oxide as indicator of antioxidant status in preeclampsia.
Study Design: Analytical case control study.
Place and Duration of Study: Sample: Department of obstretics and gynecology Department, G. M. C. Ambajogai, between July 2010 and July 2012.
Methodology: We conducted a case-control study of 57 women with preeclampsia and 57 women with uncomplicated deliveries. We measured PON1 Arylesterase activity, Nitric oxide and lipid profile.
Results: Serum levels of LDLc (low density lipoprotein cholesterol) are higher in cases than in controls and are statistically significant (p=0.023). However serum HDLc (high density lipoprotein cholesterol) levels are decreased significantly (p = 0.017). Serum PON1 Arylesterase showed significant decrease in cases152.68 KU/L versus controls 180.89 KU/L, p value=0.002. Serum nitric oxide also showed significant decrease in cases 22.77 ± 4.792 umol/L versus controls 25.127 umol/L, p=0.010. PON1 Arylesterase activity is found to be positively correlated with serum HDL cholesterol (r = 0.449, p value< 0.001). Multivariate logistic regression analysis was done.
Conclusion: Our observed results show decrease in the antioxidant PON1 Arylesterase activity point towards their role in the pathogenesis of Preeclampsia.
Brown MA, Lindheimer MD, de Swiet M, Van Assche A, Moutquin JM. The classification and diagnosis of the hypertensive disorders of pregnancy: Statement from the International Society for the Study of Hypertension in Pregnancy (ISSHP). Hypertens Pregnancy. 2001; 20(1):IX-XIV.
Lee SM, Romero R, Lee YJ, Park IS, Park CW, Yoon BH. Systemic inflammatory stimulation by microparticles derived from hypoxic trophoblast as a model for inflammatory response in preeclampsia. Am J Obstet Gynecol. 2012;207(4):337.e1-8.
Kim YJ, Park H, Lee HY, Ahn YM, Ha EH, Suh SH, Pang MG. Paraoxonase gene polymorphism, serum lipid, and oxidized low-density lipoprotein in preeclampsia. Eur J Obstet Gynecol Reprod Biol. 2007; 133(1):47-52.
Sanchez SE, Williams MA, Muy-Rivera M, Qiu C, Vadachkoria S, Bazul V. A case-control study of oxidized low density lipoproteins and preeclampsia risk. Gynecol Endocrinol. 2005;21(4):193-9.
Mackness B, Quarck R, Verreth W, Mackness M, Holvoet P. Human paraoxonase-1 over expression inhibits atherosclerosis in a mouse model of metabolic syndrome. Arterioscler Thromb Vasc Biol. 2006;26(7):1545-50.
Mackness MI, Durrington PN, Mackness B. How high-density lipoprotein protects against the effects of lipid peroxidation. Curr Opin Lipidol. 2000;11(4):383-8.
Stanger O, Weger M. Interactions of homocysteine, nitric oxide, folate and radicals in the progressively damaged endothelium. Clin Chem Lab Med. 2003; 41(11):1444-54.
Steed MM, Tyagi SC, Mechanisms of cardiovascular remodeling in hyperhomocysteinemia. Antioxid. Redox Signal. 2011;15:1927–1943.
Böger RH, Bode-Böger SM, Szuba A, Tsao PS, Chan JR, Tangphao O, Blaschke TF, Cooke JP. Asymmetric dimethylarginine (ADMA): A novel risk factor for endothelial dysfunction: Its role in hypercholesterolemia. Circulation. 1998; 98(18):1842-7.
Eckerson HW, Wyte CM, La Du BN. The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet. 1983; 35(6):1126-38.
Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide. 2001;5(1):62-71.
Hung TH, Burton GJ. Hypoxia and reoxygenation: A possible mechanism for placental oxidative stress in preeclampsia. Taiwan J Obstet Gynecol. 2006;45(3):189-200.
Aksoy AN, Ozturk N, Aksoy H, Akcay F. Paraoxonase and arylesterase activities in patients with preeclampsia The Eurasian Journal of Medicine. 2008;40:10-13.
Toy H, Camuzcuoglu H, Celik H, Erel O, Aksoy N. Assessment of serum paraoxonase and arylesterase activities in early pregnancy failure. Swiss Med Wkly. 2009;139(5-6):76-81.
Gratacós E, Casals E, Deulofeu R, Cararach V, Alonso PL, Fortuny A. Lipid peroxide and vitamin E patterns in pregnant women with different types of hypertension in pregnancy. Am J Obstet Gynecol. 1998;178(5):1072-6.
Flavahan NA. Atherosclerosis or lipoprotein-induced endothelial dysfunction. Potential mechanisms underlying reduction in EDRF/nitricoxide activity. Circulation. 1992;85(5):1927-38.
Hubel CA. Oxidative stress in the pathogenesis of preeclampsia. Proc Soc Exp Biol Med. 1999;222(3):222-35.
Mackness MI, Durrington PN. HDL, its enzymes and its potential to influence lipid peroxidation. Atherosclerosis. 1995; 115(2):243-53.
Parthasarathy S, Barnett J, Fong LG. High-density lipoprotein inhibits the oxidative modification of low-density lipoprotein. Biochim Biophys Acta. 1990; 1044(2):275-83.
Aviram M, Rosenblat M. Paraoxonases 1, 2, and 3, oxidative stress, and macrophage foam cell formation during atherosclerosis development. Free Radic Biol Med. 2004;37(9):1304-16.
Uzun H, Benian A, Madazli R, Topçuoğlu MA, Aydin S, Albayrak M. Circulating oxidized low-density lipoprotein and paraoxonase activity in preeclampsia. Gynecol Obstet Invest. 2005;60(4):195-200.
KS Meera, S Maitra, R Hemalatha Increased level of lipid peroxidation in preeclamptic pregnancy; a relationship with paraoxanase 1(PON1) activity. Biomedical Research. 2010;21:393-396.
Jakubowski H, Ambrosius WT, Pratt JH. Genetic determinants of homocysteine thiolactonase activity in humans: Implications for atherosclerosis. FEBS Lett.2001;491(1-2):35-9.
Dekker GA, van Geijn HP. Endothelial dysfunction in preeclampsia. Part II: Reducing the adverse consequences of endothelial cell dysfunction in preeclampsia; therapeutic perspectives. J Perinat Med. 1996;24(2):119-39.
Jakubowski H. Calcium-dependent human serum homocysteine thiolactone hydrolase. A protective mechanism against protein Nhomocysteinylation. J Biol Chem. 2000;275(6):3957-62.
Blatter MC, James RW, Messmer S, Barja F, Pometta D. Identification of adistinct human high-density lipoprotein subspecies defined by a lipoprotein associated protein, K-45. Identity of K-45 with paraoxonase. Eur J Biochem.1993;211(3):871–9.
Mackness MI, Arrol S, Durrington PN. Paraoxonase prevents accumulation oflipoperoxides in low-density lipoprotein. FEBS Lett. 1991;286:152–4.
Baker AM, Klein RL, Haeri S, Moss KL, Boggess KA. Association of midgestational paraoxonase 1 activity with pregnancies complicated bypreeclampsia. Am J Perinatol. 2010;27(3):205-10.
Mackness MI, Mackness B, Durrington PN, Connelly PW, Hegele RA.Paraoxonase: Biochemistry, genetics and relationship to plasma lipoproteins. CurrOpin Lipidol. 1996;7:69–76.
De Souza EM, Hirata RDC, dos Santos FCP, Picciotti R, Lucchessi AD, Silbiger V Net al Paraoxonase polymorphisms are associated with nitrate levels and vascular response in young adults with myocardial infarction. Int J Atheroscler. 2008;3(3):146-54.
Sandrim VC, Palei AC, Metzger IF, Gomes VA, Cavalli RC, Tanus- Santos JE. Nitric oxide formation is inversely related to serum levels of antiangiogenic factors soluble fms-like tyrosine kinase-1 and soluble endogline in preeclampsia. Hypertension. 2008;52(2):402-407.
Wang GR, Zhu Y, Halushka PV, Lincoln TM, Mendelsohn ME. Mechanism of platelet inhibition by nitric oxide: in vivo phosphorylation of thromboxane receptor by cyclic GMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1998;95(9): 4888-93.