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Nowadays, many medicinal plants have proved effective in combating the phenomenon of bacterial multi-resistance against conventional antibiotics. However, the use of these plants, traditionally is done without precise doses. And this inaccuracy of dose is a real problem of traditional medicine. Thus prospecting for empirically administered plant extract requires dosage monitoring to avoid the risk of a fatal therapeutic accident. It is in this context that the study of the toxicity of Terminalia macroptera which presents itself as an anti-infectious agent, capable of overcoming certain strains of antibiotic-resistant bacteria has been initiated. The objective of this study is to evaluate the toxicity of 70% ethanol extract of T.macroptera in rats and to deduce its safety. With regard to the evaluation of the toxicity, rats were used whose mass varies between 100 and 170 grams. Then, using OECD Guideline 425, (2006), acute toxicity was achieved. Then the 100, 300 and 500 mg / kg bm doses were used in sub-acute toxicity to evaluate biochemical and hematological parameters. The results show an LD50> 5000 mg / kg bm. Therefore, according to the OECD classification, the hydroethanolic extract belongs to category 5, non-toxic substances. Also, the biochemical and hematological results revealed that the extract did not change at any time at P <0.05, biochemical marker levels (UREE, ASAT, ALAT, CK and LDH), reflecting vital organs of the body. So the extract would have no effect on the heart, liver and kidneys. 70% ethanol extract of T. macroptera would be safe for use as a drug and therefore could contribute to the production of Traditionally Enhanced Medicines (MTAs).
Walsh C. Antibiotics: Actions, origins, resistance. Washington, D.C., Amer Society for Microbiology. 2003;335.
Mougeot C, Guillaumat-Taillet Y, Libert JM. Staphylococcus aureus: New detection of intrinsic resistance by the diffusion method, Patholbiol. 2001;49:199-204.
Kolling M, Winkley K, Von Deden M. For someone who’s rich, it’s not a problem. Insights from Tanzania on diabete health-seeking and medical pluralism among Dar es-salam’s urban poor. Globalization and Health. 2010;6:8-11.
Mangambou M. Taxonomy, biogeography and ecology of pteridophytes in the mountain forest ecosystem of the Kohuzi-Biega National Park in eastern DRC. Congo. Doctoral thesis University d’Anvers. (Belgique). 2013;463.
Nacoulma O. Medicinal plants and traditional medicinal practices of Burkina Faso: Case of the central plateau. PhD Thesis in Natural Sciences, University of Ouagadougou. (Burkina.Faso). Faculty. Science and Technology. 1996 ;605.
N’Guessand K. Medicinal plants taxonomy, biogeography and ecology of the pterido-phytes of the mountain forest ecosystem of the Kohuzi-Biega National Park east of the R.D.Congo. PhD thesis and traditional medical practices among Abbey and krobou peoples of the department of agboville (Ivory Coast). PhD thesis in natural sciences. University of Cocody. Abidjan. UFR., Biosciences, Botany Laboratory. 2008;235.
Bruneton J. Pharmacognosy: Phyto-chemistry, medicinal plants. Tec & Lavoisier. Paris. 2009;778.
Adjoungoua A, Diafouka F, Koffi P, Lokrou A, Attah I. Valorization of the traditional pharmacopoeia the action of the ethanolic extant of Bidens pilosa (Asteraceae) on the static and dynamic exploration of glycemia. Journal of Medicine and African Pharmacopoeia. 2006;19:1-12.
Bagré I, Bahi C, Ouattara K, Zirihi GN, Djaman AJ, Coulibaly A, N’guessan JD. Botanical study and exploration of the antifungal activity of Morinda morindoides (BAKER) MiRedh, on the in vitro growth of Cryptococcus neoformans, Herbal Medicine. 2011;9:136-141.
GN Zihiri, AKM Kra. Evaluation of microglossa pirifilia antifungal activity (LARMARCK) O. KUNTZE (Asteraceae) "PYMI" on in vitro growth of Candida albicans. Rev. Med. Pharm. Afr. 2003;17: 11-19.
Bagre I, Bahi C, Gnahoue G, Djaman AJ, Guédé GF. Phytochemical composition and in vitro evaluation of the antifungal activity of Morinda morindoides leaf extracts (Baker) Milne-Redhead (Rubiaceae) on Aspergillus fumigatus and Candida albicans. Journal of Pharmaco-logical and Biological Sciences. 2007;8 : 15-23.
Ayan M, Kuzucu C, Durmaz R, Aktas E, Cizmeci Z. Analysis of three outbreaks due to Klebsiella species in a neonatal intensive care unit. Infection control and hospital Epidemilogy. 2003;24:495-500.
Tang JJ, Li JG, Qiuww, Lips Li BL, Song BL. Inhibition of SREBP by a small molecule, betulin, improves hyperlipidemia and insulin resistance and reduces atherosclerotic plaques. Metabolite Cell. 2011;13:46-56.
OCDE. OECD Guideline for the Testing of Chemicals. 2008b;407.
Bidié AP, Yapo FA, Yéo D, N’guessan JD, Djaman AJ, Guédé-Guina F. Effect of Mitragyna ciliata (MYTA) on the rat cardiovascular system. Phytotherapy. 2010;8:1-6.
Haeckel R. Simplified determinations of the “True” creatinine concentration in serum and urine. J Clin Chem Clin Biochem. 1980;18:385-394.
Jaynes PK. An enzymatic, reaction-rate assay for serum creatinine with a centrifugal analyzer. 1982;28:114-117.
Yayi YG, Ackah JAAB, Kra AKM, Djaman JA. Antifungal activity of different extracts of Terminalia mantaly, H. Perrier on the in vitro growth of Aspergillus fumigatus. European Journal of Medical Research. 2012;82:132-138.
Bouayed I. Study of some biochemical parameters in patients with coronary syndrome. Final dissertation. Department of Biology, option: Cellular phytopathology. University of Tlemencen, Rep. Algerian Democratic and Popular; 2013.
Djédjé H. Prospective study for the reduction and stabilization of glycemia in diabeticrabbits by DIACODA, substances from plant sources. Memory of D.E.A. Pharmacology of Natural Substances Université de Cocody. Abidjan. 2002; 34.
Young L. Role of the ABC transporter Mdl1 in peptide export from mitochondria. Science. 2001;291(5511):2135-8.
Bleyere NM, Ekaza JD, Angoue YP, Datte JY, Banga NB Cathy NMA, Vanga M, Kone M, Ehouan EE. Heterogeneity of Iron level from woman during the pregnancy in Côte d’Ivoire. Ann. Biol. Clin. 2007;65(5):525-532. French
Silva EJR, Concalves ES, Aguiar FJS, Evencio LB, Lyra MMA, Coelho MCOC, Fraga MCCA, Wanderley AG. Toxicological studies on hydroalcool extract of Calendula officinalis L. PhytotherapyResearch. 2007; 21:332-336.
Wink M. The plant vacuole: A multi-functional compartment. J. EXP. Botany. 1993 ;44(Supplement):231-246.
Fourrier F, Chopin C, Huart JJ, Runge I, Caron C, Goudemand J. Double-blind, placebo-controlled trial of antithrombin III concentrates in septic shock with disseminated intravascular coagulation. Journal of the American College of Chest Physicians. 1993;104 :882-888.
OCDE. OECD Guideline for the Testing of Chemicals: Acute Oral Toxicity - Method of Dose Addition. OECD. 2006;425:29.
Adeneye AA, Benebo AS. Ameliorating the effects of acetaminophen-induced hepato-toxicity in rats with African red palm oil extract. Asian Journal of Traditional Medicine. 2007;2:244-249.
Hilaly JE, Israili ZH, Lyouss B. Acute and chronic toxicological studies of Ajuva Iva in experimental animals. Journal of Ethno-pharmacology. 2004;91:43-50.
Gazda VE, Gomes-Caeneiro MR, Barbi NS, Paumgartten FJR. Toxicological evaluation of an ethanolic extract from Chiococca alba roots. Journal of Traditional Medicine. 2006;105:187-195.
Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A. Concordance of toxicity of pharmaceuticals in humans and in animals. Regulatory Toxicology and Pharmacology. 2000;32:56-67.
Chang-Gue S, Seung-Hyun H, Jung-Hyo C, Jang-Woo S, Chin-Ho C, Yeon-Weol I, Chong-Kwan C. Induction of hemopoiesis by saenghyuldan, a mixture of Ginseng radix, Paeoniae radix abla, and hominis placenta extracts. Acta Pharmaco-logiasonica. 2003;24:120-126.
Bain BJ. Blood cell a practice guide. Ed Blackwell, Australia. 2006;71-89.
Okada Yasunori, Morodomi Tatsuhisa, J Jan, Engkild, Suzuki KO, Yasui Atsuhi, Nakanishi Isuo, Salvesen Guy, Nagase Hideaki. Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts. European Journal of Biochemistry Banner. 1990;194(3):721-730.
Coulibaly A, Koné T, Ouattara NI, N’Douba V, Snoeks J, Kouamélan EP, Gouré BIG. Evaluation of a man-made shelter’s effects on survival and growth of Hetero-branchus longifilis fry under cage culture. Belg. J. Zool. 2007;137(2):157-164.
Tang JJ, Li JG, Qiuww Lips Li BL, Song BL. Inhibition of SREBP by a small molecule, betulin, improves hyperlipidemia and insulin resistance and reduces atherosclerotic plaques. Metabolite Cell. 2011;13:46-56.
Rhiouani H, El-Hilaly J, IsrailiZ H, Badiaa Lyouss B. Acute and sub-chronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents. Journal of Ethnopharmacology. 2008;118:667-677.
Tulsawani R. Ninety day repeated gavage administration of Hipphophaerhamnoides extract in rats. Food and Chemical Toxicology. 2010;48:2483–2489.
Adeneye AA, Ajagbonna OP, Adeleke TI, Bello SO. Preliminary toxicity and phytochemical studies of the stem bark aqueous extract of Musanga cecropioides in rats. J. Ethnopharmacol. 2006;105(3): 374-9.
Wolf PL, William D, Tsudaka T, Acosta T. Methods and technics in clinical chemistry, John Wiley & Son, USA. 1972;132-196.
Sakly R, Hamadou M, Ennerifer A. Action of white mugwort on blood sugar, cholerosterolemia and triglyceridemia in
normal and diabetic subjects. Journal of Medicine and African Pharcopoeia. 1991; 5:29-39.
Ouedraogo PA, Sou S, Sanou A, Monge JP, Huignard J, Tran B, Credland PF. Influence of temperature and humidity on populations of Callosobruchus maculatus (Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Pteromalidae) in two climatic zones of Burkina Faso. Bulletin of Entomological Research. 1996;6:692-702.